Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1658 and GFF1659 are separated by 236 nucleotides GFF1659 and GFF1660 overlap by 1 nucleotides GFF1660 and GFF1661 overlap by 8 nucleotides
GFF1658 - Rhamnogalacturonides degradation protein RhiN, at 42,865 to 44,004
GFF1658
GFF1659 - Flagellar protein FlhE, at 44,241 to 44,633
GFF1659
GFF1660 - Flagellar biosynthesis protein FlhA, at 44,633 to 46,711
GFF1660
GFF1661 - Flagellar biosynthesis protein FlhB, at 46,704 to 47,855
GFF1661
Position (kb) 44
45
46
47 Strain fitness (log2 ratio) -1
0
1
2 at 43.781 kb on + strand, within GFF1658 at 43.781 kb on + strand, within GFF1658 at 43.781 kb on + strand, within GFF1658 at 43.782 kb on - strand, within GFF1658 at 44.129 kb on + strand at 44.129 kb on + strand at 44.292 kb on - strand, within GFF1659 at 44.542 kb on - strand, within GFF1659 at 44.547 kb on + strand, within GFF1659 at 44.547 kb on + strand, within GFF1659 at 44.548 kb on - strand, within GFF1659 at 44.742 kb on - strand at 44.967 kb on - strand, within GFF1660 at 44.978 kb on + strand, within GFF1660 at 45.080 kb on + strand, within GFF1660 at 45.641 kb on + strand, within GFF1660 at 45.642 kb on - strand, within GFF1660 at 46.105 kb on + strand, within GFF1660 at 46.234 kb on + strand, within GFF1660 at 46.234 kb on + strand, within GFF1660 at 46.574 kb on + strand at 46.715 kb on + strand at 46.715 kb on + strand at 46.847 kb on - strand, within GFF1661 at 46.848 kb on + strand, within GFF1661 at 46.848 kb on + strand, within GFF1661 at 46.888 kb on + strand, within GFF1661 at 46.891 kb on - strand, within GFF1661 at 46.969 kb on - strand, within GFF1661 at 47.135 kb on + strand, within GFF1661 at 47.454 kb on - strand, within GFF1661 at 47.540 kb on + strand, within GFF1661 at 47.541 kb on - strand, within GFF1661
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 43,781 + GFF1658 0.80 +0.4 43,781 + GFF1658 0.80 +1.2 43,781 + GFF1658 0.80 +0.4 43,782 - GFF1658 0.80 -0.6 44,129 + +0.9 44,129 + +0.5 44,292 - GFF1659 0.13 +0.3 44,542 - GFF1659 0.77 +0.9 44,547 + GFF1659 0.78 +0.5 44,547 + GFF1659 0.78 +0.2 44,548 - GFF1659 0.78 -0.2 44,742 - +0.9 44,967 - GFF1660 0.16 +1.3 44,978 + GFF1660 0.17 +1.2 45,080 + GFF1660 0.22 +1.4 45,641 + GFF1660 0.48 +1.7 45,642 - GFF1660 0.49 +1.3 46,105 + GFF1660 0.71 -0.7 46,234 + GFF1660 0.77 +0.4 46,234 + GFF1660 0.77 +1.7 46,574 + +2.2 46,715 + +1.9 46,715 + +1.5 46,847 - GFF1661 0.12 +1.5 46,848 + GFF1661 0.12 +1.7 46,848 + GFF1661 0.12 +1.1 46,888 + GFF1661 0.16 +1.4 46,891 - GFF1661 0.16 +0.7 46,969 - GFF1661 0.23 +1.7 47,135 + GFF1661 0.37 +1.4 47,454 - GFF1661 0.65 +0.9 47,540 + GFF1661 0.73 +2.3 47,541 - GFF1661 0.73 +0.4
Or see this region's nucleotide sequence