Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1635 and GFF1636 are separated by 15 nucleotides GFF1636 and GFF1637 are separated by 78 nucleotides GFF1637 and GFF1638 overlap by 4 nucleotides
GFF1635 - Cell wall endopeptidase, family M23/M37, at 21,501 to 22,820
GFF1635
GFF1636 - Zinc ABC transporter, periplasmic-binding protein ZnuA, at 22,836 to 23,780
GFF1636
GFF1637 - Zinc ABC transporter, ATP-binding protein ZnuC, at 23,859 to 24,614
GFF1637
GFF1638 - Zinc ABC transporter, inner membrane permease protein ZnuB, at 24,611 to 25,396
GFF1638
Position (kb) 22
23
24 Strain fitness (log2 ratio) -2
-1
0
1
2 at 21.970 kb on - strand, within GFF1635 at 22.006 kb on + strand, within GFF1635 at 22.040 kb on - strand, within GFF1635 at 22.040 kb on - strand, within GFF1635 at 22.040 kb on - strand, within GFF1635 at 22.242 kb on - strand, within GFF1635 at 22.242 kb on - strand, within GFF1635 at 22.254 kb on - strand, within GFF1635 at 22.331 kb on - strand, within GFF1635 at 22.461 kb on - strand, within GFF1635 at 22.463 kb on + strand, within GFF1635 at 22.529 kb on - strand, within GFF1635 at 22.583 kb on + strand, within GFF1635 at 22.673 kb on + strand, within GFF1635 at 22.822 kb on + strand at 22.873 kb on - strand at 22.905 kb on - strand at 23.131 kb on + strand, within GFF1636 at 23.349 kb on - strand, within GFF1636 at 23.371 kb on - strand, within GFF1636 at 23.371 kb on - strand, within GFF1636 at 23.484 kb on - strand, within GFF1636 at 23.550 kb on + strand, within GFF1636 at 23.790 kb on - strand at 23.999 kb on - strand, within GFF1637 at 24.069 kb on + strand, within GFF1637 at 24.070 kb on - strand, within GFF1637 at 24.087 kb on + strand, within GFF1637 at 24.133 kb on + strand, within GFF1637 at 24.336 kb on + strand, within GFF1637 at 24.400 kb on + strand, within GFF1637 at 24.449 kb on + strand, within GFF1637 at 24.449 kb on + strand, within GFF1637 at 24.450 kb on - strand, within GFF1637 at 24.450 kb on - strand, within GFF1637 at 24.503 kb on + strand, within GFF1637
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 21,970 - GFF1635 0.36 +0.5 22,006 + GFF1635 0.38 +0.3 22,040 - GFF1635 0.41 +0.6 22,040 - GFF1635 0.41 +0.6 22,040 - GFF1635 0.41 +0.3 22,242 - GFF1635 0.56 -0.1 22,242 - GFF1635 0.56 +1.2 22,254 - GFF1635 0.57 +0.2 22,331 - GFF1635 0.63 +0.7 22,461 - GFF1635 0.73 -0.4 22,463 + GFF1635 0.73 +0.9 22,529 - GFF1635 0.78 +0.4 22,583 + GFF1635 0.82 +1.2 22,673 + GFF1635 0.89 +0.8 22,822 + +0.3 22,873 - -2.1 22,905 - -1.1 23,131 + GFF1636 0.31 +1.0 23,349 - GFF1636 0.54 -0.5 23,371 - GFF1636 0.57 +0.0 23,371 - GFF1636 0.57 -0.2 23,484 - GFF1636 0.69 -0.1 23,550 + GFF1636 0.76 +0.6 23,790 - +0.4 23,999 - GFF1637 0.19 +1.2 24,069 + GFF1637 0.28 -0.3 24,070 - GFF1637 0.28 +0.1 24,087 + GFF1637 0.30 +0.3 24,133 + GFF1637 0.36 +0.0 24,336 + GFF1637 0.63 +0.5 24,400 + GFF1637 0.72 +0.4 24,449 + GFF1637 0.78 -0.2 24,449 + GFF1637 0.78 -0.5 24,450 - GFF1637 0.78 +1.8 24,450 - GFF1637 0.78 +0.3 24,503 + GFF1637 0.85 +0.1
Or see this region's nucleotide sequence