Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1481 and GFF1482 are separated by 355 nucleotides GFF1482 and GFF1483 are separated by 13 nucleotides GFF1483 and GFF1484 overlap by 8 nucleotides
GFF1481 - Fumarate respiration sensor kinase protein DcuS, at 150,024 to 151,655
GFF1481
GFF1482 - Anaerobic dimethyl sulfoxide reductase chain A (EC 1.8.99.-), at 152,011 to 154,440
GFF1482
GFF1483 - Anaerobic dimethyl sulfoxide reductase chain B (EC 1.8.5.3), at 154,454 to 155,080
GFF1483
GFF1484 - Anaerobic dimethyl sulfoxide reductase chain C (EC 1.8.5.3), at 155,073 to 155,846
GFF1484
Position (kb) 152
153
154
155 Strain fitness (log2 ratio) -2
-1
0
1
2 at 151.061 kb on - strand, within GFF1481 at 151.071 kb on + strand, within GFF1481 at 151.107 kb on - strand, within GFF1481 at 151.379 kb on + strand, within GFF1481 at 151.493 kb on + strand at 151.689 kb on - strand at 151.707 kb on - strand at 152.418 kb on + strand, within GFF1482 at 152.559 kb on - strand, within GFF1482 at 152.632 kb on + strand, within GFF1482 at 152.648 kb on - strand, within GFF1482 at 152.791 kb on - strand, within GFF1482 at 152.893 kb on - strand, within GFF1482 at 152.998 kb on - strand, within GFF1482 at 153.004 kb on + strand, within GFF1482 at 153.128 kb on - strand, within GFF1482 at 153.129 kb on + strand, within GFF1482 at 153.130 kb on - strand, within GFF1482 at 153.130 kb on - strand, within GFF1482 at 153.130 kb on - strand, within GFF1482 at 153.130 kb on - strand, within GFF1482 at 153.258 kb on + strand, within GFF1482 at 153.291 kb on + strand, within GFF1482 at 153.550 kb on + strand, within GFF1482 at 153.551 kb on + strand, within GFF1482 at 153.581 kb on - strand, within GFF1482 at 153.635 kb on - strand, within GFF1482 at 153.689 kb on - strand, within GFF1482 at 153.736 kb on + strand, within GFF1482 at 153.736 kb on + strand, within GFF1482 at 153.738 kb on + strand, within GFF1482 at 153.913 kb on - strand, within GFF1482 at 153.913 kb on - strand, within GFF1482 at 154.111 kb on - strand, within GFF1482 at 154.124 kb on - strand, within GFF1482 at 154.226 kb on + strand at 154.451 kb on - strand at 154.472 kb on + strand at 154.473 kb on - strand at 154.574 kb on + strand, within GFF1483 at 154.575 kb on - strand, within GFF1483 at 154.627 kb on + strand, within GFF1483 at 154.627 kb on + strand, within GFF1483 at 154.628 kb on - strand, within GFF1483 at 154.631 kb on + strand, within GFF1483 at 154.632 kb on - strand, within GFF1483 at 154.635 kb on - strand, within GFF1483 at 154.651 kb on + strand, within GFF1483 at 154.746 kb on + strand, within GFF1483 at 154.938 kb on - strand, within GFF1483 at 155.000 kb on - strand, within GFF1483 at 155.002 kb on + strand, within GFF1483 at 155.108 kb on + strand at 155.437 kb on + strand, within GFF1484
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 151,061 - GFF1481 0.64 -0.6 151,071 + GFF1481 0.64 -0.1 151,107 - GFF1481 0.66 -0.9 151,379 + GFF1481 0.83 +0.6 151,493 + +0.4 151,689 - -0.2 151,707 - -0.1 152,418 + GFF1482 0.17 +0.5 152,559 - GFF1482 0.23 -0.6 152,632 + GFF1482 0.26 -2.1 152,648 - GFF1482 0.26 +0.1 152,791 - GFF1482 0.32 -0.4 152,893 - GFF1482 0.36 +0.1 152,998 - GFF1482 0.41 -0.2 153,004 + GFF1482 0.41 -0.7 153,128 - GFF1482 0.46 +0.2 153,129 + GFF1482 0.46 +0.1 153,130 - GFF1482 0.46 +0.9 153,130 - GFF1482 0.46 +0.7 153,130 - GFF1482 0.46 +0.7 153,130 - GFF1482 0.46 +0.6 153,258 + GFF1482 0.51 -0.6 153,291 + GFF1482 0.53 -0.7 153,550 + GFF1482 0.63 -0.1 153,551 + GFF1482 0.63 +1.8 153,581 - GFF1482 0.65 -0.1 153,635 - GFF1482 0.67 +1.4 153,689 - GFF1482 0.69 +0.1 153,736 + GFF1482 0.71 +0.1 153,736 + GFF1482 0.71 +0.3 153,738 + GFF1482 0.71 -0.0 153,913 - GFF1482 0.78 +0.3 153,913 - GFF1482 0.78 +1.0 154,111 - GFF1482 0.86 +0.4 154,124 - GFF1482 0.87 -1.9 154,226 + -0.2 154,451 - +0.8 154,472 + -0.2 154,473 - +0.5 154,574 + GFF1483 0.19 +1.9 154,575 - GFF1483 0.19 +0.4 154,627 + GFF1483 0.28 -0.3 154,627 + GFF1483 0.28 +0.2 154,628 - GFF1483 0.28 +0.3 154,631 + GFF1483 0.28 +0.3 154,632 - GFF1483 0.28 -1.0 154,635 - GFF1483 0.29 +0.6 154,651 + GFF1483 0.31 -0.4 154,746 + GFF1483 0.47 +0.8 154,938 - GFF1483 0.77 -0.0 155,000 - GFF1483 0.87 -0.7 155,002 + GFF1483 0.87 +0.2 155,108 + +0.5 155,437 + GFF1484 0.47 +0.6
Or see this region's nucleotide sequence