Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1474 and GFF1475 are separated by 310 nucleotides GFF1475 and GFF1476 are separated by 83 nucleotides
GFF1474 - Melibiose operon regulatory protein, at 141,328 to 142,218
GFF1474
GFF1475 - Alpha-galactosidase (EC 3.2.1.22), at 142,529 to 143,884
GFF1475
GFF1476 - Melibiose carrier protein, Na+/melibiose symporter, at 143,968 to 145,398
GFF1476
Position (kb)
142
143
144 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 141.543 kb on + strand, within GFF1474 at 141.570 kb on - strand, within GFF1474 at 141.607 kb on - strand, within GFF1474 at 141.627 kb on - strand, within GFF1474 at 141.753 kb on + strand, within GFF1474 at 141.847 kb on - strand, within GFF1474 at 141.901 kb on - strand, within GFF1474 at 142.020 kb on + strand, within GFF1474 at 142.062 kb on - strand, within GFF1474 at 142.097 kb on - strand, within GFF1474 at 142.173 kb on - strand at 142.347 kb on + strand at 142.347 kb on + strand at 142.375 kb on + strand at 142.376 kb on - strand at 142.376 kb on - strand at 142.431 kb on + strand at 142.432 kb on - strand at 142.512 kb on + strand at 142.513 kb on - strand at 142.513 kb on - strand at 142.736 kb on - strand, within GFF1475 at 142.835 kb on - strand, within GFF1475 at 143.009 kb on + strand, within GFF1475 at 143.009 kb on + strand, within GFF1475 at 143.010 kb on - strand, within GFF1475 at 143.010 kb on - strand, within GFF1475 at 143.056 kb on - strand, within GFF1475 at 143.093 kb on - strand, within GFF1475 at 143.183 kb on + strand, within GFF1475 at 143.184 kb on - strand, within GFF1475 at 143.357 kb on - strand, within GFF1475 at 143.427 kb on + strand, within GFF1475 at 143.450 kb on - strand, within GFF1475 at 143.468 kb on - strand, within GFF1475 at 143.522 kb on - strand, within GFF1475 at 143.522 kb on - strand, within GFF1475 at 143.572 kb on + strand, within GFF1475 at 143.762 kb on + strand at 143.875 kb on - strand at 143.926 kb on - strand at 143.926 kb on - strand at 143.928 kb on + strand at 143.967 kb on + strand at 143.999 kb on - strand at 144.043 kb on + strand at 144.043 kb on + strand at 144.043 kb on + strand at 144.044 kb on - strand at 144.044 kb on - strand at 144.045 kb on + strand at 144.045 kb on + strand at 144.045 kb on + strand at 144.046 kb on - strand at 144.046 kb on - strand at 144.046 kb on - strand at 144.049 kb on + strand at 144.049 kb on + strand at 144.049 kb on + strand at 144.049 kb on + strand at 144.050 kb on - strand at 144.050 kb on - strand at 144.050 kb on - strand at 144.059 kb on - strand at 144.059 kb on - strand at 144.062 kb on - strand at 144.287 kb on + strand, within GFF1476 at 144.287 kb on + strand, within GFF1476 at 144.288 kb on - strand, within GFF1476 at 144.304 kb on + strand, within GFF1476 at 144.304 kb on + strand, within GFF1476 at 144.325 kb on + strand, within GFF1476 at 144.326 kb on - strand, within GFF1476 at 144.327 kb on + strand, within GFF1476 at 144.327 kb on + strand, within GFF1476 at 144.328 kb on - strand, within GFF1476 at 144.367 kb on - strand, within GFF1476 at 144.369 kb on + strand, within GFF1476 at 144.452 kb on + strand, within GFF1476 at 144.472 kb on + strand, within GFF1476 at 144.513 kb on + strand, within GFF1476 at 144.521 kb on + strand, within GFF1476 at 144.569 kb on + strand, within GFF1476 at 144.569 kb on + strand, within GFF1476 at 144.570 kb on - strand, within GFF1476 at 144.570 kb on - strand, within GFF1476 at 144.570 kb on - strand, within GFF1476 at 144.600 kb on - strand, within GFF1476 at 144.712 kb on - strand, within GFF1476 at 144.737 kb on - strand, within GFF1476 at 144.746 kb on - strand, within GFF1476 at 144.746 kb on - strand, within GFF1476 at 144.778 kb on + strand, within GFF1476
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 141,543 + GFF1474 0.24 -0.4 141,570 - GFF1474 0.27 -0.3 141,607 - GFF1474 0.31 -0.1 141,627 - GFF1474 0.34 +0.0 141,753 + GFF1474 0.48 -0.1 141,847 - GFF1474 0.58 +0.2 141,901 - GFF1474 0.64 -0.1 142,020 + GFF1474 0.78 +0.4 142,062 - GFF1474 0.82 +0.2 142,097 - GFF1474 0.86 -0.8 142,173 - +1.2 142,347 + -1.4 142,347 + -0.6 142,375 + -0.2 142,376 - -0.0 142,376 - +0.1 142,431 + +0.1 142,432 - -0.1 142,512 + +0.4 142,513 - -0.0 142,513 - -0.5 142,736 - GFF1475 0.15 -0.3 142,835 - GFF1475 0.23 -0.5 143,009 + GFF1475 0.35 -0.1 143,009 + GFF1475 0.35 +0.3 143,010 - GFF1475 0.35 -0.2 143,010 - GFF1475 0.35 -0.6 143,056 - GFF1475 0.39 +1.1 143,093 - GFF1475 0.42 -0.8 143,183 + GFF1475 0.48 -0.8 143,184 - GFF1475 0.48 -0.1 143,357 - GFF1475 0.61 +1.1 143,427 + GFF1475 0.66 -1.1 143,450 - GFF1475 0.68 -0.2 143,468 - GFF1475 0.69 -0.4 143,522 - GFF1475 0.73 -1.1 143,522 - GFF1475 0.73 -0.0 143,572 + GFF1475 0.77 +0.2 143,762 + -0.2 143,875 - -0.2 143,926 - +0.4 143,926 - +0.2 143,928 + -0.4 143,967 + -3.5 143,999 - +0.4 144,043 + -0.1 144,043 + -0.5 144,043 + +0.2 144,044 - -0.7 144,044 - +1.2 144,045 + +2.0 144,045 + +0.9 144,045 + +0.1 144,046 - +0.5 144,046 - -1.0 144,046 - +0.3 144,049 + -1.0 144,049 + +0.7 144,049 + -0.4 144,049 + +0.8 144,050 - -0.1 144,050 - +0.3 144,050 - -0.5 144,059 - +1.3 144,059 - -0.5 144,062 - -0.0 144,287 + GFF1476 0.22 +1.4 144,287 + GFF1476 0.22 -0.9 144,288 - GFF1476 0.22 +0.2 144,304 + GFF1476 0.23 +0.1 144,304 + GFF1476 0.23 -0.7 144,325 + GFF1476 0.25 -0.6 144,326 - GFF1476 0.25 +0.3 144,327 + GFF1476 0.25 -0.2 144,327 + GFF1476 0.25 -0.5 144,328 - GFF1476 0.25 -2.1 144,367 - GFF1476 0.28 +0.1 144,369 + GFF1476 0.28 -0.3 144,452 + GFF1476 0.34 -0.1 144,472 + GFF1476 0.35 +0.4 144,513 + GFF1476 0.38 -0.2 144,521 + GFF1476 0.39 -1.1 144,569 + GFF1476 0.42 +0.9 144,569 + GFF1476 0.42 +0.3 144,570 - GFF1476 0.42 -0.0 144,570 - GFF1476 0.42 +0.4 144,570 - GFF1476 0.42 -0.6 144,600 - GFF1476 0.44 -0.5 144,712 - GFF1476 0.52 -0.2 144,737 - GFF1476 0.54 +0.8 144,746 - GFF1476 0.54 +1.4 144,746 - GFF1476 0.54 -0.4 144,778 + GFF1476 0.57 +0.4
Or see this region's nucleotide sequence