Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1453 and GFF1454 are separated by 111 nucleotides GFF1454 and GFF1455 overlap by 4 nucleotides GFF1455 and GFF1456 overlap by 4 nucleotides GFF1456 and GFF1457 overlap by 8 nucleotides
GFF1453 - Cytochrome c552 precursor (EC 1.7.2.2), at 116,235 to 117,671
GFF1453
GFF1454 - Cytochrome c-type protein NrfB precursor, at 117,783 to 118,349
GFF1454
GFF1455 - NrfC protein, at 118,346 to 119,017
GFF1455
GFF1456 - NrfD protein, at 119,014 to 119,970
GFF1456
GFF1457 - Cytochrome c heme lyase subunit CcmF, at 119,963 to 122,185
GFF1457
Position (kb)
118
119
120 Strain fitness (log2 ratio)
-2
-1
0
1 at 117.408 kb on - strand, within GFF1453 at 117.408 kb on - strand, within GFF1453 at 117.474 kb on - strand, within GFF1453 at 117.575 kb on + strand at 117.576 kb on - strand at 117.737 kb on - strand at 117.766 kb on + strand at 117.767 kb on - strand at 117.771 kb on - strand at 117.771 kb on - strand at 117.782 kb on + strand at 117.783 kb on - strand at 117.803 kb on - strand at 117.998 kb on + strand, within GFF1454 at 118.093 kb on - strand, within GFF1454 at 118.093 kb on - strand, within GFF1454 at 118.095 kb on - strand, within GFF1454 at 118.100 kb on - strand, within GFF1454 at 118.121 kb on + strand, within GFF1454 at 118.122 kb on - strand, within GFF1454 at 118.122 kb on - strand, within GFF1454 at 118.441 kb on + strand, within GFF1455 at 118.559 kb on - strand, within GFF1455 at 118.769 kb on - strand, within GFF1455 at 118.795 kb on + strand, within GFF1455 at 118.940 kb on + strand, within GFF1455 at 118.994 kb on - strand at 118.997 kb on + strand at 118.998 kb on - strand at 119.075 kb on - strand at 119.164 kb on - strand, within GFF1456 at 119.164 kb on - strand, within GFF1456 at 119.275 kb on + strand, within GFF1456 at 119.323 kb on + strand, within GFF1456 at 119.330 kb on + strand, within GFF1456 at 119.330 kb on + strand, within GFF1456 at 119.331 kb on - strand, within GFF1456 at 119.466 kb on - strand, within GFF1456 at 119.497 kb on + strand, within GFF1456 at 119.545 kb on - strand, within GFF1456 at 119.631 kb on + strand, within GFF1456 at 119.632 kb on - strand, within GFF1456 at 119.632 kb on - strand, within GFF1456 at 119.663 kb on + strand, within GFF1456 at 119.687 kb on + strand, within GFF1456 at 119.687 kb on + strand, within GFF1456 at 119.688 kb on - strand, within GFF1456 at 119.688 kb on - strand, within GFF1456 at 119.866 kb on - strand, within GFF1456
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 117,408 - GFF1453 0.82 -0.9 117,408 - GFF1453 0.82 -0.9 117,474 - GFF1453 0.86 -0.3 117,575 + -0.5 117,576 - -0.8 117,737 - -0.0 117,766 + +0.4 117,767 - -1.6 117,771 - -2.3 117,771 - +0.3 117,782 + +0.0 117,783 - +0.0 117,803 - -0.1 117,998 + GFF1454 0.38 +0.1 118,093 - GFF1454 0.55 -1.1 118,093 - GFF1454 0.55 -0.5 118,095 - GFF1454 0.55 -0.6 118,100 - GFF1454 0.56 -0.3 118,121 + GFF1454 0.60 -0.2 118,122 - GFF1454 0.60 -0.3 118,122 - GFF1454 0.60 +0.6 118,441 + GFF1455 0.14 -0.1 118,559 - GFF1455 0.32 -0.8 118,769 - GFF1455 0.63 -1.0 118,795 + GFF1455 0.67 -0.1 118,940 + GFF1455 0.88 -0.1 118,994 - -0.1 118,997 + -0.3 118,998 - +0.4 119,075 - -1.1 119,164 - GFF1456 0.16 -0.2 119,164 - GFF1456 0.16 +0.6 119,275 + GFF1456 0.27 -0.1 119,323 + GFF1456 0.32 +0.1 119,330 + GFF1456 0.33 -0.1 119,330 + GFF1456 0.33 +0.8 119,331 - GFF1456 0.33 -0.4 119,466 - GFF1456 0.47 +0.1 119,497 + GFF1456 0.50 +0.1 119,545 - GFF1456 0.55 -0.1 119,631 + GFF1456 0.64 -0.4 119,632 - GFF1456 0.65 -1.2 119,632 - GFF1456 0.65 +0.0 119,663 + GFF1456 0.68 -0.2 119,687 + GFF1456 0.70 -0.7 119,687 + GFF1456 0.70 -0.4 119,688 - GFF1456 0.70 +0.0 119,688 - GFF1456 0.70 +0.9 119,866 - GFF1456 0.89 +0.7
Or see this region's nucleotide sequence