Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1452 and GFF1453 are separated by 431 nucleotides GFF1453 and GFF1454 are separated by 111 nucleotides GFF1454 and GFF1455 overlap by 4 nucleotides
GFF1452 - Acetyl-coenzyme A synthetase (EC 6.2.1.1), at 113,845 to 115,803
GFF1452
GFF1453 - Cytochrome c552 precursor (EC 1.7.2.2), at 116,235 to 117,671
GFF1453
GFF1454 - Cytochrome c-type protein NrfB precursor, at 117,783 to 118,349
GFF1454
GFF1455 - NrfC protein, at 118,346 to 119,017
GFF1455
Position (kb)
116
117
118 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 115.235 kb on - strand, within GFF1452 at 115.235 kb on - strand, within GFF1452 at 115.392 kb on - strand, within GFF1452 at 115.417 kb on - strand, within GFF1452 at 115.482 kb on + strand, within GFF1452 at 115.483 kb on - strand, within GFF1452 at 115.483 kb on - strand, within GFF1452 at 115.483 kb on - strand, within GFF1452 at 115.519 kb on - strand, within GFF1452 at 115.603 kb on - strand, within GFF1452 at 115.733 kb on + strand at 115.865 kb on - strand at 115.922 kb on - strand at 116.087 kb on + strand at 116.134 kb on + strand at 116.159 kb on - strand at 116.209 kb on - strand at 116.276 kb on + strand at 116.279 kb on - strand at 116.881 kb on - strand, within GFF1453 at 116.909 kb on + strand, within GFF1453 at 116.955 kb on + strand, within GFF1453 at 116.966 kb on + strand, within GFF1453 at 117.137 kb on + strand, within GFF1453 at 117.277 kb on + strand, within GFF1453 at 117.278 kb on - strand, within GFF1453 at 117.278 kb on - strand, within GFF1453 at 117.408 kb on - strand, within GFF1453 at 117.408 kb on - strand, within GFF1453 at 117.474 kb on - strand, within GFF1453 at 117.575 kb on + strand at 117.576 kb on - strand at 117.737 kb on - strand at 117.766 kb on + strand at 117.767 kb on - strand at 117.771 kb on - strand at 117.771 kb on - strand at 117.782 kb on + strand at 117.783 kb on - strand at 117.803 kb on - strand at 117.998 kb on + strand, within GFF1454 at 118.093 kb on - strand, within GFF1454 at 118.093 kb on - strand, within GFF1454 at 118.095 kb on - strand, within GFF1454 at 118.100 kb on - strand, within GFF1454 at 118.121 kb on + strand, within GFF1454 at 118.122 kb on - strand, within GFF1454 at 118.122 kb on - strand, within GFF1454 at 118.441 kb on + strand, within GFF1455 at 118.559 kb on - strand, within GFF1455
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 115,235 - GFF1452 0.71 +0.3 115,235 - GFF1452 0.71 +0.9 115,392 - GFF1452 0.79 +0.2 115,417 - GFF1452 0.80 +2.0 115,482 + GFF1452 0.84 -0.1 115,483 - GFF1452 0.84 -0.5 115,483 - GFF1452 0.84 +0.0 115,483 - GFF1452 0.84 +0.5 115,519 - GFF1452 0.85 -0.6 115,603 - GFF1452 0.90 +0.1 115,733 + -0.4 115,865 - -0.7 115,922 - +0.2 116,087 + +0.0 116,134 + -0.2 116,159 - +0.7 116,209 - +0.4 116,276 + -0.1 116,279 - -0.1 116,881 - GFF1453 0.45 +1.0 116,909 + GFF1453 0.47 +0.8 116,955 + GFF1453 0.50 -0.5 116,966 + GFF1453 0.51 -0.7 117,137 + GFF1453 0.63 -0.4 117,277 + GFF1453 0.73 -0.2 117,278 - GFF1453 0.73 -0.9 117,278 - GFF1453 0.73 +0.2 117,408 - GFF1453 0.82 -0.9 117,408 - GFF1453 0.82 -0.9 117,474 - GFF1453 0.86 -0.3 117,575 + -0.5 117,576 - -0.8 117,737 - -0.0 117,766 + +0.4 117,767 - -1.6 117,771 - -2.3 117,771 - +0.3 117,782 + +0.0 117,783 - +0.0 117,803 - -0.1 117,998 + GFF1454 0.38 +0.1 118,093 - GFF1454 0.55 -1.1 118,093 - GFF1454 0.55 -0.5 118,095 - GFF1454 0.55 -0.6 118,100 - GFF1454 0.56 -0.3 118,121 + GFF1454 0.60 -0.2 118,122 - GFF1454 0.60 -0.3 118,122 - GFF1454 0.60 +0.6 118,441 + GFF1455 0.14 -0.1 118,559 - GFF1455 0.32 -0.8
Or see this region's nucleotide sequence