Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1376 and GFF1377 are separated by 8 nucleotides GFF1377 and GFF1378 overlap by 4 nucleotides GFF1378 and GFF1379 are separated by 298 nucleotides GFF1379 and GFF1380 are separated by 8 nucleotides
GFF1376 - putative inner membrane protein, at 24,574 to 26,112
GFF1376
GFF1377 - Polymyxin resistance protein ArnC, glycosyl transferase (EC 2.4.-.-), at 26,121 to 27,050
GFF1377
GFF1378 - putative phage glucose translocase, at 27,047 to 27,304
GFF1378
GFF1379 - hypothetical protein, at 27,603 to 27,728
GFF1379
GFF1380 - Putative phage baseplate component, at 27,737 to 28,459
GFF1380
Position (kb) 26
27
28 Strain fitness (log2 ratio) -1
0
1
2
3 at 25.143 kb on + strand, within GFF1376 at 25.144 kb on - strand, within GFF1376 at 25.233 kb on + strand, within GFF1376 at 25.233 kb on + strand, within GFF1376 at 25.234 kb on - strand, within GFF1376 at 25.238 kb on - strand, within GFF1376 at 25.238 kb on - strand, within GFF1376 at 25.238 kb on - strand, within GFF1376 at 25.257 kb on + strand, within GFF1376 at 25.334 kb on + strand, within GFF1376 at 25.334 kb on + strand, within GFF1376 at 25.335 kb on - strand, within GFF1376 at 25.462 kb on + strand, within GFF1376 at 25.499 kb on + strand, within GFF1376 at 25.802 kb on - strand, within GFF1376 at 25.969 kb on - strand at 25.970 kb on + strand at 25.971 kb on - strand at 26.009 kb on + strand at 26.080 kb on + strand at 26.080 kb on + strand at 26.198 kb on - strand at 26.323 kb on + strand, within GFF1377 at 26.324 kb on - strand, within GFF1377 at 26.324 kb on - strand, within GFF1377 at 26.359 kb on + strand, within GFF1377 at 26.545 kb on - strand, within GFF1377 at 26.638 kb on + strand, within GFF1377 at 26.658 kb on - strand, within GFF1377 at 26.730 kb on - strand, within GFF1377 at 26.943 kb on - strand, within GFF1377 at 27.143 kb on - strand, within GFF1378 at 27.201 kb on + strand, within GFF1378 at 27.210 kb on + strand, within GFF1378 at 27.224 kb on + strand, within GFF1378 at 27.226 kb on - strand, within GFF1378 at 27.388 kb on - strand at 27.399 kb on - strand at 27.536 kb on + strand at 27.540 kb on + strand at 27.540 kb on + strand at 27.812 kb on - strand, within GFF1380 at 27.984 kb on - strand, within GFF1380 at 27.984 kb on - strand, within GFF1380
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 25,143 + GFF1376 0.37 +0.1 25,144 - GFF1376 0.37 -0.1 25,233 + GFF1376 0.43 -0.7 25,233 + GFF1376 0.43 -0.3 25,234 - GFF1376 0.43 -0.1 25,238 - GFF1376 0.43 +1.0 25,238 - GFF1376 0.43 +0.1 25,238 - GFF1376 0.43 +0.2 25,257 + GFF1376 0.44 -0.3 25,334 + GFF1376 0.49 -0.1 25,334 + GFF1376 0.49 +0.1 25,335 - GFF1376 0.49 -0.7 25,462 + GFF1376 0.58 +1.4 25,499 + GFF1376 0.60 -0.1 25,802 - GFF1376 0.80 -0.6 25,969 - -0.1 25,970 + -0.7 25,971 - +1.2 26,009 + +0.8 26,080 + -0.3 26,080 + -0.3 26,198 - -1.6 26,323 + GFF1377 0.22 +3.6 26,324 - GFF1377 0.22 -0.2 26,324 - GFF1377 0.22 -1.0 26,359 + GFF1377 0.26 -1.6 26,545 - GFF1377 0.46 -0.0 26,638 + GFF1377 0.56 -0.6 26,658 - GFF1377 0.58 -0.1 26,730 - GFF1377 0.65 -0.0 26,943 - GFF1377 0.88 +0.0 27,143 - GFF1378 0.37 -0.2 27,201 + GFF1378 0.60 -1.0 27,210 + GFF1378 0.63 +0.3 27,224 + GFF1378 0.69 -0.3 27,226 - GFF1378 0.69 -0.3 27,388 - -0.6 27,399 - -0.9 27,536 + +0.3 27,540 + -0.2 27,540 + -0.4 27,812 - GFF1380 0.10 +0.2 27,984 - GFF1380 0.34 -0.4 27,984 - GFF1380 0.34 -1.3
Or see this region's nucleotide sequence