Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF127

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF126 and GFF127 overlap by 1 nucleotidesGFF127 and GFF128 are separated by 91 nucleotidesGFF128 and GFF129 overlap by 4 nucleotides GFF126 - Possible GPH family transporter (TC 2.A.2) for arabinosides, at 115,919 to 117,280 GFF126 GFF127 - Alpha-L-arabinofuranosidase II precursor (EC 3.2.1.55), at 117,280 to 118,230 GFF127 GFF128 - AmpE protein, at 118,322 to 119,176 GFF128 GFF129 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) AmpD, at 119,173 to 119,736 GFF129 Position (kb) 117 118 119Strain fitness (log2 ratio) -3 -2 -1 0 1at 116.459 kb on - strand, within GFF126at 116.459 kb on - strand, within GFF126at 116.569 kb on - strand, within GFF126at 116.581 kb on - strand, within GFF126at 116.589 kb on + strand, within GFF126at 116.589 kb on + strand, within GFF126at 116.589 kb on + strand, within GFF126at 116.590 kb on - strand, within GFF126at 116.590 kb on - strand, within GFF126at 116.594 kb on - strand, within GFF126at 116.612 kb on - strand, within GFF126at 116.633 kb on + strand, within GFF126at 116.633 kb on + strand, within GFF126at 116.634 kb on - strand, within GFF126at 116.634 kb on - strand, within GFF126at 116.634 kb on - strand, within GFF126at 116.634 kb on - strand, within GFF126at 116.636 kb on + strand, within GFF126at 116.637 kb on - strand, within GFF126at 116.637 kb on - strand, within GFF126at 117.094 kb on + strand, within GFF126at 117.094 kb on + strand, within GFF126at 117.095 kb on - strand, within GFF126at 117.095 kb on - strand, within GFF126at 117.095 kb on - strand, within GFF126at 117.129 kb on - strand, within GFF126at 117.151 kb on - strandat 117.328 kb on - strandat 117.333 kb on - strandat 117.333 kb on - strandat 117.349 kb on + strandat 117.353 kb on - strandat 117.357 kb on + strandat 117.451 kb on - strand, within GFF127at 117.526 kb on + strand, within GFF127at 117.559 kb on - strand, within GFF127at 117.897 kb on + strand, within GFF127at 117.904 kb on + strand, within GFF127at 117.959 kb on + strand, within GFF127at 117.993 kb on + strand, within GFF127at 117.994 kb on - strand, within GFF127at 118.062 kb on + strand, within GFF127at 118.228 kb on + strandat 118.328 kb on + strandat 118.328 kb on + strandat 118.339 kb on - strandat 118.418 kb on + strand, within GFF128at 118.479 kb on + strand, within GFF128at 118.479 kb on + strand, within GFF128at 118.489 kb on + strand, within GFF128at 118.490 kb on - strand, within GFF128at 118.490 kb on - strand, within GFF128at 118.554 kb on + strand, within GFF128at 118.554 kb on + strand, within GFF128at 118.560 kb on - strand, within GFF128at 118.808 kb on - strand, within GFF128at 118.808 kb on - strand, within GFF128at 118.905 kb on + strand, within GFF128at 118.906 kb on - strand, within GFF128at 118.911 kb on - strand, within GFF128at 118.934 kb on - strand, within GFF128at 118.983 kb on - strand, within GFF128at 118.994 kb on + strand, within GFF128at 119.084 kb on + strand, within GFF128at 119.156 kb on + strandat 119.164 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
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116,459 - GFF126 0.40 -0.3
116,459 - GFF126 0.40 +1.4
116,569 - GFF126 0.48 +0.1
116,581 - GFF126 0.49 +0.2
116,589 + GFF126 0.49 -1.0
116,589 + GFF126 0.49 -1.0
116,589 + GFF126 0.49 +0.9
116,590 - GFF126 0.49 +1.6
116,590 - GFF126 0.49 +0.7
116,594 - GFF126 0.50 -0.1
116,612 - GFF126 0.51 +0.5
116,633 + GFF126 0.52 -0.5
116,633 + GFF126 0.52 -0.2
116,634 - GFF126 0.52 +0.2
116,634 - GFF126 0.52 -0.6
116,634 - GFF126 0.52 +0.4
116,634 - GFF126 0.52 -3.0
116,636 + GFF126 0.53 -0.1
116,637 - GFF126 0.53 -0.3
116,637 - GFF126 0.53 +0.6
117,094 + GFF126 0.86 +0.0
117,094 + GFF126 0.86 +0.2
117,095 - GFF126 0.86 -0.1
117,095 - GFF126 0.86 +0.0
117,095 - GFF126 0.86 -0.1
117,129 - GFF126 0.89 -0.2
117,151 - -0.1
117,328 - -0.8
117,333 - -0.9
117,333 - +0.5
117,349 + -0.4
117,353 - -0.4
117,357 + +0.0
117,451 - GFF127 0.18 +0.6
117,526 + GFF127 0.26 +0.5
117,559 - GFF127 0.29 +0.6
117,897 + GFF127 0.65 +0.1
117,904 + GFF127 0.66 -0.3
117,959 + GFF127 0.71 -0.4
117,993 + GFF127 0.75 +0.8
117,994 - GFF127 0.75 +0.4
118,062 + GFF127 0.82 +0.3
118,228 + +1.3
118,328 + -1.3
118,328 + -0.1
118,339 - +0.1
118,418 + GFF128 0.11 +0.1
118,479 + GFF128 0.18 -0.3
118,479 + GFF128 0.18 -0.4
118,489 + GFF128 0.20 +0.3
118,490 - GFF128 0.20 -0.5
118,490 - GFF128 0.20 -0.0
118,554 + GFF128 0.27 +0.4
118,554 + GFF128 0.27 +0.3
118,560 - GFF128 0.28 +0.9
118,808 - GFF128 0.57 -0.2
118,808 - GFF128 0.57 +0.0
118,905 + GFF128 0.68 +0.1
118,906 - GFF128 0.68 -0.5
118,911 - GFF128 0.69 -0.1
118,934 - GFF128 0.72 +0.1
118,983 - GFF128 0.77 -0.1
118,994 + GFF128 0.79 +0.3
119,084 + GFF128 0.89 +1.6
119,156 + -0.2
119,164 + -0.1

Or see this region's nucleotide sequence