Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1230 and GFF1231 are separated by 23 nucleotides GFF1231 and GFF1232 are separated by 66 nucleotides GFF1232 and GFF1233 are separated by 322 nucleotides
GFF1230 - Salmonella enterica serovar Choleraesuis 50k virulence plasmid DNA, complete sequence, at 64,567 to 65,025
GFF1230
GFF1231 - Invasin, at 65,049 to 65,465
GFF1231
GFF1232 - Transposase, at 65,532 to 65,798
GFF1232
GFF1233 - Transposase, at 66,121 to 66,363
GFF1233
Position (kb) 65
66 Strain fitness (log2 ratio) -1
0
1
2 at 64.573 kb on + strand at 64.573 kb on + strand at 64.573 kb on + strand at 64.574 kb on - strand at 64.574 kb on - strand at 64.576 kb on - strand at 64.741 kb on - strand, within GFF1230 at 64.978 kb on - strand, within GFF1230 at 64.994 kb on + strand at 65.013 kb on + strand at 65.014 kb on - strand at 65.014 kb on - strand at 65.014 kb on - strand at 65.014 kb on - strand at 65.104 kb on - strand, within GFF1231 at 65.142 kb on + strand, within GFF1231 at 65.143 kb on - strand, within GFF1231 at 65.151 kb on + strand, within GFF1231 at 65.151 kb on + strand, within GFF1231 at 65.568 kb on - strand, within GFF1232 at 65.648 kb on + strand, within GFF1232 at 65.657 kb on - strand, within GFF1232 at 65.657 kb on - strand, within GFF1232 at 65.657 kb on - strand, within GFF1232 at 65.675 kb on - strand, within GFF1232 at 65.720 kb on + strand, within GFF1232 at 65.720 kb on + strand, within GFF1232 at 65.721 kb on - strand, within GFF1232 at 65.761 kb on + strand, within GFF1232 at 65.778 kb on - strand at 65.855 kb on - strand at 65.856 kb on + strand at 65.856 kb on + strand at 65.856 kb on + strand at 65.856 kb on + strand at 65.857 kb on - strand at 65.857 kb on - strand at 65.857 kb on - strand at 65.857 kb on - strand at 65.863 kb on - strand at 65.900 kb on - strand at 65.986 kb on + strand at 65.992 kb on - strand at 66.068 kb on + strand at 66.196 kb on - strand, within GFF1233 at 66.287 kb on - strand, within GFF1233 at 66.318 kb on - strand, within GFF1233 at 66.334 kb on + strand, within GFF1233 at 66.335 kb on - strand, within GFF1233 at 66.335 kb on - strand, within GFF1233 at 66.397 kb on + strand at 66.398 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 64,573 + +1.9 64,573 + -0.7 64,573 + +0.3 64,574 - +0.1 64,574 - +0.5 64,576 - +1.2 64,741 - GFF1230 0.38 +0.7 64,978 - GFF1230 0.90 +1.9 64,994 + +0.6 65,013 + +2.2 65,014 - +1.6 65,014 - +0.6 65,014 - +1.2 65,014 - +1.2 65,104 - GFF1231 0.13 +1.0 65,142 + GFF1231 0.22 +0.9 65,143 - GFF1231 0.23 +0.6 65,151 + GFF1231 0.24 +0.6 65,151 + GFF1231 0.24 +0.5 65,568 - GFF1232 0.13 +1.5 65,648 + GFF1232 0.43 +0.5 65,657 - GFF1232 0.47 +0.5 65,657 - GFF1232 0.47 +0.8 65,657 - GFF1232 0.47 +0.2 65,675 - GFF1232 0.54 +0.8 65,720 + GFF1232 0.70 +0.1 65,720 + GFF1232 0.70 -0.7 65,721 - GFF1232 0.71 +0.1 65,761 + GFF1232 0.86 -0.8 65,778 - +1.1 65,855 - -0.3 65,856 + +0.1 65,856 + +0.0 65,856 + -0.0 65,856 + -1.4 65,857 - +0.6 65,857 - +0.3 65,857 - +0.9 65,857 - -0.9 65,863 - +0.4 65,900 - -0.1 65,986 + +0.9 65,992 - -0.1 66,068 + -0.6 66,196 - GFF1233 0.31 +0.1 66,287 - GFF1233 0.68 -0.8 66,318 - GFF1233 0.81 -0.2 66,334 + GFF1233 0.88 -1.6 66,335 - GFF1233 0.88 -0.9 66,335 - GFF1233 0.88 -0.3 66,397 + -0.3 66,398 - +0.3
Or see this region's nucleotide sequence