Experiment: LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1031 and GFF1032 are separated by 102 nucleotides GFF1032 and GFF1033 are separated by 94 nucleotides GFF1033 and GFF1034 are separated by 21 nucleotides GFF1034 and GFF1035 are separated by 91 nucleotides
GFF1031 - Ribosomal RNA small subunit methyltransferase C (EC 2.1.1.52), at 214,490 to 215,518
GFF1031
GFF1032 - DNA polymerase III psi subunit (EC 2.7.7.7), at 215,621 to 216,058
GFF1032
GFF1033 - Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-), at 216,153 to 216,449
GFF1033
GFF1034 - '5'-nucleotidase YjjG (EC 3.1.3.5)' transl_table=11, at 216,471 to 217,148
GFF1034
GFF1035 - Peptide chain release factor 3, at 217,240 to 218,829
GFF1035
Position (kb) 216
217
218 Strain fitness (log2 ratio) -1
0
1
2
3 at 215.552 kb on + strand at 215.553 kb on - strand at 215.553 kb on - strand at 215.555 kb on + strand at 215.556 kb on - strand at 215.588 kb on + strand at 215.659 kb on + strand at 215.662 kb on + strand at 215.757 kb on + strand, within GFF1032 at 215.876 kb on - strand, within GFF1032 at 216.131 kb on + strand at 216.132 kb on - strand at 216.132 kb on - strand at 216.170 kb on + strand at 216.248 kb on + strand, within GFF1033 at 216.296 kb on - strand, within GFF1033 at 216.296 kb on - strand, within GFF1033 at 216.314 kb on - strand, within GFF1033 at 216.345 kb on + strand, within GFF1033 at 216.387 kb on + strand, within GFF1033 at 216.440 kb on - strand at 216.448 kb on - strand at 216.467 kb on - strand at 216.516 kb on - strand at 216.628 kb on - strand, within GFF1034 at 216.652 kb on + strand, within GFF1034 at 216.728 kb on + strand, within GFF1034 at 216.729 kb on - strand, within GFF1034 at 216.782 kb on + strand, within GFF1034 at 216.783 kb on - strand, within GFF1034 at 216.783 kb on - strand, within GFF1034 at 216.804 kb on - strand, within GFF1034 at 216.815 kb on + strand, within GFF1034 at 216.815 kb on + strand, within GFF1034 at 216.818 kb on + strand, within GFF1034 at 216.818 kb on + strand, within GFF1034 at 216.838 kb on + strand, within GFF1034 at 216.986 kb on - strand, within GFF1034 at 217.140 kb on - strand at 217.294 kb on - strand at 217.377 kb on - strand at 217.441 kb on - strand, within GFF1035 at 217.534 kb on - strand, within GFF1035 at 217.537 kb on + strand, within GFF1035 at 217.537 kb on + strand, within GFF1035 at 217.612 kb on - strand, within GFF1035 at 217.777 kb on - strand, within GFF1035 at 217.795 kb on + strand, within GFF1035 at 217.796 kb on - strand, within GFF1035 at 217.796 kb on - strand, within GFF1035 at 217.810 kb on + strand, within GFF1035 at 217.810 kb on + strand, within GFF1035 at 217.974 kb on - strand, within GFF1035 at 218.040 kb on + strand, within GFF1035
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt_new) 2 MOI remove 215,552 + +0.9 215,553 - +0.3 215,553 - +1.2 215,555 + +0.3 215,556 - +0.8 215,588 + -0.3 215,659 + +1.0 215,662 + -0.6 215,757 + GFF1032 0.31 +0.6 215,876 - GFF1032 0.58 +0.3 216,131 + +0.6 216,132 - -0.7 216,132 - +1.2 216,170 + -0.6 216,248 + GFF1033 0.32 +0.0 216,296 - GFF1033 0.48 +0.6 216,296 - GFF1033 0.48 -0.5 216,314 - GFF1033 0.54 -1.1 216,345 + GFF1033 0.65 -1.3 216,387 + GFF1033 0.79 +0.0 216,440 - +0.1 216,448 - +0.5 216,467 - -0.1 216,516 - +0.8 216,628 - GFF1034 0.23 +1.3 216,652 + GFF1034 0.27 -0.4 216,728 + GFF1034 0.38 -0.1 216,729 - GFF1034 0.38 -1.0 216,782 + GFF1034 0.46 +0.6 216,783 - GFF1034 0.46 +0.7 216,783 - GFF1034 0.46 -0.4 216,804 - GFF1034 0.49 +1.0 216,815 + GFF1034 0.51 +0.3 216,815 + GFF1034 0.51 -0.1 216,818 + GFF1034 0.51 +0.6 216,818 + GFF1034 0.51 +0.4 216,838 + GFF1034 0.54 -0.6 216,986 - GFF1034 0.76 +0.8 217,140 - +0.0 217,294 - +1.9 217,377 - +1.9 217,441 - GFF1035 0.13 +0.8 217,534 - GFF1035 0.18 +1.9 217,537 + GFF1035 0.19 +1.3 217,537 + GFF1035 0.19 -0.8 217,612 - GFF1035 0.23 +1.8 217,777 - GFF1035 0.34 +1.9 217,795 + GFF1035 0.35 +1.6 217,796 - GFF1035 0.35 +2.1 217,796 - GFF1035 0.35 +1.6 217,810 + GFF1035 0.36 +3.2 217,810 + GFF1035 0.36 +2.4 217,974 - GFF1035 0.46 +1.8 218,040 + GFF1035 0.50 +1.8
Or see this region's nucleotide sequence