Experiment: Fusidic 0.03 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_653 and PfGW456L13_654 are separated by 158 nucleotides PfGW456L13_654 and PfGW456L13_655 are separated by 0 nucleotides PfGW456L13_655 and PfGW456L13_656 are separated by 159 nucleotides PfGW456L13_656 and PfGW456L13_657 are separated by 40 nucleotides PfGW456L13_657 and PfGW456L13_658 are separated by 81 nucleotides
PfGW456L13_653 - Trk system potassium uptake protein TrkA, at 743,740 to 745,116
_653
PfGW456L13_654 - Chaperone protein YscY (Yop proteins translocation protein Y), at 745,275 to 745,589
_654
PfGW456L13_655 - FIG00954045: hypothetical protein, at 745,590 to 745,829
_655
PfGW456L13_656 - Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-), at 745,989 to 746,876
_656
PfGW456L13_657 - DNA-3-methyladenine glycosylase (EC 3.2.2.20), at 746,917 to 747,474
_657
PfGW456L13_658 - Glycyl-tRNA synthetase alpha chain (EC 6.1.1.14), at 747,556 to 748,509
_658
Position (kb)
745
746
747 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 745.068 kb on + strand at 745.068 kb on + strand at 745.080 kb on - strand at 745.080 kb on - strand at 745.080 kb on - strand at 745.080 kb on - strand at 745.080 kb on - strand at 745.132 kb on + strand at 745.135 kb on + strand at 745.170 kb on + strand at 745.170 kb on + strand at 745.230 kb on + strand at 745.235 kb on + strand at 745.235 kb on + strand at 745.235 kb on + strand at 745.235 kb on + strand at 745.235 kb on + strand at 745.235 kb on + strand at 745.236 kb on - strand at 745.236 kb on - strand at 745.236 kb on - strand at 745.236 kb on - strand at 745.236 kb on - strand at 745.236 kb on - strand at 745.350 kb on + strand, within PfGW456L13_654 at 745.351 kb on - strand, within PfGW456L13_654 at 745.351 kb on - strand, within PfGW456L13_654 at 745.419 kb on + strand, within PfGW456L13_654 at 745.420 kb on - strand, within PfGW456L13_654 at 745.438 kb on + strand, within PfGW456L13_654 at 745.597 kb on + strand at 745.598 kb on - strand at 745.665 kb on + strand, within PfGW456L13_655 at 745.665 kb on + strand, within PfGW456L13_655 at 745.666 kb on - strand, within PfGW456L13_655 at 745.666 kb on - strand, within PfGW456L13_655 at 745.666 kb on - strand, within PfGW456L13_655 at 745.685 kb on - strand, within PfGW456L13_655 at 745.733 kb on - strand, within PfGW456L13_655 at 745.733 kb on - strand, within PfGW456L13_655 at 745.733 kb on - strand, within PfGW456L13_655 at 745.733 kb on - strand, within PfGW456L13_655 at 745.733 kb on - strand, within PfGW456L13_655 at 745.859 kb on + strand at 745.859 kb on + strand at 745.860 kb on - strand at 745.993 kb on + strand at 746.047 kb on + strand at 746.047 kb on + strand at 746.048 kb on - strand at 746.048 kb on - strand at 746.072 kb on - strand at 746.125 kb on + strand, within PfGW456L13_656 at 746.125 kb on + strand, within PfGW456L13_656 at 746.125 kb on + strand, within PfGW456L13_656 at 746.126 kb on - strand, within PfGW456L13_656 at 746.126 kb on - strand, within PfGW456L13_656 at 746.486 kb on - strand, within PfGW456L13_656 at 746.486 kb on - strand, within PfGW456L13_656 at 746.774 kb on + strand, within PfGW456L13_656 at 746.774 kb on + strand, within PfGW456L13_656 at 746.775 kb on - strand, within PfGW456L13_656 at 746.919 kb on - strand at 747.428 kb on + strand at 747.428 kb on + strand at 747.429 kb on - strand at 747.437 kb on + strand at 747.437 kb on + strand at 747.437 kb on + strand at 747.437 kb on + strand at 747.437 kb on + strand at 747.437 kb on + strand at 747.437 kb on + strand at 747.437 kb on + strand at 747.437 kb on + strand at 747.437 kb on + strand at 747.475 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.03 mg/ml remove 745,068 + +0.9 745,068 + +0.1 745,080 - +0.7 745,080 - +0.8 745,080 - -0.9 745,080 - -0.2 745,080 - +0.1 745,132 + -0.1 745,135 + -0.1 745,170 + +0.3 745,170 + -0.8 745,230 + +0.6 745,235 + +0.3 745,235 + -0.2 745,235 + -0.4 745,235 + -0.6 745,235 + +0.2 745,235 + +0.3 745,236 - +1.4 745,236 - +0.4 745,236 - +0.3 745,236 - -0.6 745,236 - -0.3 745,236 - +0.7 745,350 + PfGW456L13_654 0.24 +0.3 745,351 - PfGW456L13_654 0.24 +0.2 745,351 - PfGW456L13_654 0.24 -0.2 745,419 + PfGW456L13_654 0.46 -1.4 745,420 - PfGW456L13_654 0.46 +0.4 745,438 + PfGW456L13_654 0.52 -0.4 745,597 + +1.7 745,598 - +0.3 745,665 + PfGW456L13_655 0.31 -0.4 745,665 + PfGW456L13_655 0.31 -0.2 745,666 - PfGW456L13_655 0.32 +0.1 745,666 - PfGW456L13_655 0.32 -0.6 745,666 - PfGW456L13_655 0.32 -0.2 745,685 - PfGW456L13_655 0.40 -0.6 745,733 - PfGW456L13_655 0.60 +0.1 745,733 - PfGW456L13_655 0.60 -1.4 745,733 - PfGW456L13_655 0.60 +0.3 745,733 - PfGW456L13_655 0.60 -1.3 745,733 - PfGW456L13_655 0.60 +0.6 745,859 + -0.0 745,859 + +1.1 745,860 - +0.2 745,993 + -2.9 746,047 + -1.6 746,047 + -2.3 746,048 - -2.1 746,048 - -2.6 746,072 - -0.9 746,125 + PfGW456L13_656 0.15 -0.1 746,125 + PfGW456L13_656 0.15 -1.9 746,125 + PfGW456L13_656 0.15 +0.4 746,126 - PfGW456L13_656 0.15 -1.6 746,126 - PfGW456L13_656 0.15 -2.9 746,486 - PfGW456L13_656 0.56 -1.1 746,486 - PfGW456L13_656 0.56 -1.1 746,774 + PfGW456L13_656 0.88 +0.4 746,774 + PfGW456L13_656 0.88 -1.7 746,775 - PfGW456L13_656 0.89 -1.1 746,919 - +0.5 747,428 + +0.7 747,428 + -0.0 747,429 - -0.4 747,437 + -1.9 747,437 + -1.8 747,437 + -1.3 747,437 + +0.7 747,437 + +0.4 747,437 + +0.3 747,437 + +0.2 747,437 + +0.3 747,437 + -1.7 747,437 + +0.2 747,475 - +0.1
Or see this region's nucleotide sequence