Experiment: Fusidic 0.03 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_3071 and PfGW456L13_3072 are separated by 29 nucleotides PfGW456L13_3072 and PfGW456L13_3073 are separated by 71 nucleotides
PfGW456L13_3071 - O-acetylhomoserine sulfhydrylase (EC 2.5.1.49), at 3,418,557 to 3,419,963
_3071
PfGW456L13_3072 - Nucleoside-diphosphate-sugar epimerases, at 3,419,993 to 3,420,334
_3072
PfGW456L13_3073 - Putrescine ABC transporter, periplasmic putrescine-binding protein, at 3,420,406 to 3,421,491
_3073
Position (kb)
3419
3420
3421 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3419.018 kb on - strand, within PfGW456L13_3071 at 3419.018 kb on - strand, within PfGW456L13_3071 at 3419.043 kb on - strand, within PfGW456L13_3071 at 3419.097 kb on + strand, within PfGW456L13_3071 at 3419.097 kb on + strand, within PfGW456L13_3071 at 3419.098 kb on - strand, within PfGW456L13_3071 at 3419.098 kb on - strand, within PfGW456L13_3071 at 3419.098 kb on - strand, within PfGW456L13_3071 at 3419.098 kb on - strand, within PfGW456L13_3071 at 3419.181 kb on + strand, within PfGW456L13_3071 at 3419.181 kb on + strand, within PfGW456L13_3071 at 3419.221 kb on + strand, within PfGW456L13_3071 at 3419.221 kb on + strand, within PfGW456L13_3071 at 3419.222 kb on - strand, within PfGW456L13_3071 at 3419.222 kb on - strand, within PfGW456L13_3071 at 3419.270 kb on - strand, within PfGW456L13_3071 at 3419.277 kb on + strand, within PfGW456L13_3071 at 3419.278 kb on - strand, within PfGW456L13_3071 at 3419.308 kb on - strand, within PfGW456L13_3071 at 3419.425 kb on - strand, within PfGW456L13_3071 at 3419.527 kb on + strand, within PfGW456L13_3071 at 3419.527 kb on + strand, within PfGW456L13_3071 at 3419.527 kb on + strand, within PfGW456L13_3071 at 3419.527 kb on + strand, within PfGW456L13_3071 at 3419.527 kb on + strand, within PfGW456L13_3071 at 3419.527 kb on + strand, within PfGW456L13_3071 at 3419.528 kb on - strand, within PfGW456L13_3071 at 3419.541 kb on + strand, within PfGW456L13_3071 at 3419.541 kb on + strand at 3419.841 kb on + strand at 3419.962 kb on - strand at 3419.962 kb on - strand at 3419.974 kb on + strand at 3419.974 kb on + strand at 3419.974 kb on + strand at 3419.975 kb on - strand at 3419.975 kb on - strand at 3419.987 kb on - strand at 3419.987 kb on - strand at 3419.987 kb on - strand at 3419.987 kb on - strand at 3420.283 kb on + strand, within PfGW456L13_3072 at 3420.284 kb on - strand, within PfGW456L13_3072 at 3420.317 kb on + strand at 3420.318 kb on - strand at 3420.318 kb on - strand at 3420.318 kb on - strand at 3420.318 kb on - strand at 3420.378 kb on + strand at 3420.378 kb on + strand at 3420.378 kb on + strand at 3420.391 kb on + strand at 3420.392 kb on - strand at 3420.484 kb on + strand at 3420.484 kb on + strand at 3420.485 kb on - strand at 3420.487 kb on - strand at 3420.499 kb on + strand at 3420.500 kb on - strand at 3420.500 kb on - strand at 3420.556 kb on + strand, within PfGW456L13_3073 at 3420.556 kb on + strand, within PfGW456L13_3073 at 3420.557 kb on - strand, within PfGW456L13_3073 at 3420.557 kb on - strand, within PfGW456L13_3073 at 3420.557 kb on - strand, within PfGW456L13_3073 at 3420.557 kb on - strand, within PfGW456L13_3073 at 3420.557 kb on - strand, within PfGW456L13_3073 at 3420.557 kb on - strand, within PfGW456L13_3073 at 3420.557 kb on - strand, within PfGW456L13_3073 at 3420.557 kb on - strand, within PfGW456L13_3073 at 3420.566 kb on - strand, within PfGW456L13_3073 at 3420.566 kb on - strand, within PfGW456L13_3073 at 3420.779 kb on - strand, within PfGW456L13_3073 at 3420.806 kb on - strand, within PfGW456L13_3073 at 3420.806 kb on - strand, within PfGW456L13_3073 at 3420.959 kb on - strand, within PfGW456L13_3073 at 3420.997 kb on + strand, within PfGW456L13_3073 at 3420.997 kb on + strand at 3420.998 kb on - strand, within PfGW456L13_3073 at 3420.998 kb on - strand, within PfGW456L13_3073 at 3421.036 kb on + strand, within PfGW456L13_3073 at 3421.037 kb on - strand, within PfGW456L13_3073 at 3421.037 kb on - strand at 3421.037 kb on - strand, within PfGW456L13_3073 at 3421.037 kb on - strand, within PfGW456L13_3073 at 3421.045 kb on + strand, within PfGW456L13_3073 at 3421.046 kb on - strand, within PfGW456L13_3073 at 3421.046 kb on - strand, within PfGW456L13_3073 at 3421.046 kb on - strand, within PfGW456L13_3073 at 3421.046 kb on - strand, within PfGW456L13_3073 at 3421.063 kb on + strand, within PfGW456L13_3073 at 3421.064 kb on - strand, within PfGW456L13_3073 at 3421.064 kb on - strand, within PfGW456L13_3073 at 3421.064 kb on - strand, within PfGW456L13_3073 at 3421.064 kb on - strand, within PfGW456L13_3073 at 3421.064 kb on - strand, within PfGW456L13_3073 at 3421.064 kb on - strand, within PfGW456L13_3073 at 3421.263 kb on + strand, within PfGW456L13_3073 at 3421.263 kb on + strand, within PfGW456L13_3073 at 3421.264 kb on - strand, within PfGW456L13_3073 at 3421.270 kb on + strand, within PfGW456L13_3073 at 3421.271 kb on - strand, within PfGW456L13_3073 at 3421.309 kb on + strand, within PfGW456L13_3073 at 3421.309 kb on + strand, within PfGW456L13_3073 at 3421.309 kb on + strand, within PfGW456L13_3073 at 3421.309 kb on + strand, within PfGW456L13_3073 at 3421.310 kb on - strand, within PfGW456L13_3073 at 3421.310 kb on - strand, within PfGW456L13_3073 at 3421.310 kb on - strand, within PfGW456L13_3073 at 3421.310 kb on - strand, within PfGW456L13_3073 at 3421.310 kb on - strand, within PfGW456L13_3073 at 3421.310 kb on - strand, within PfGW456L13_3073
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.03 mg/ml remove 3,419,018 - PfGW456L13_3071 0.33 +0.6 3,419,018 - PfGW456L13_3071 0.33 -0.1 3,419,043 - PfGW456L13_3071 0.35 -2.4 3,419,097 + PfGW456L13_3071 0.38 -0.5 3,419,097 + PfGW456L13_3071 0.38 +2.4 3,419,098 - PfGW456L13_3071 0.38 -1.0 3,419,098 - PfGW456L13_3071 0.38 -0.7 3,419,098 - PfGW456L13_3071 0.38 -0.7 3,419,098 - PfGW456L13_3071 0.38 -0.1 3,419,181 + PfGW456L13_3071 0.44 +0.7 3,419,181 + PfGW456L13_3071 0.44 -0.1 3,419,221 + PfGW456L13_3071 0.47 -0.1 3,419,221 + PfGW456L13_3071 0.47 +0.2 3,419,222 - PfGW456L13_3071 0.47 +0.1 3,419,222 - PfGW456L13_3071 0.47 +0.6 3,419,270 - PfGW456L13_3071 0.51 -0.6 3,419,277 + PfGW456L13_3071 0.51 +0.2 3,419,278 - PfGW456L13_3071 0.51 +0.0 3,419,308 - PfGW456L13_3071 0.53 +0.5 3,419,425 - PfGW456L13_3071 0.62 +0.6 3,419,527 + PfGW456L13_3071 0.69 +0.1 3,419,527 + PfGW456L13_3071 0.69 +0.3 3,419,527 + PfGW456L13_3071 0.69 -1.0 3,419,527 + PfGW456L13_3071 0.69 -0.4 3,419,527 + PfGW456L13_3071 0.69 +1.9 3,419,527 + PfGW456L13_3071 0.69 -0.0 3,419,528 - PfGW456L13_3071 0.69 -1.0 3,419,541 + PfGW456L13_3071 0.70 -0.8 3,419,541 + +0.6 3,419,841 + +1.4 3,419,962 - +1.8 3,419,962 - -0.6 3,419,974 + +0.2 3,419,974 + -3.2 3,419,974 + +1.7 3,419,975 - +1.0 3,419,975 - +0.0 3,419,987 - -0.1 3,419,987 - +0.2 3,419,987 - +0.3 3,419,987 - +0.1 3,420,283 + PfGW456L13_3072 0.85 -1.4 3,420,284 - PfGW456L13_3072 0.85 +1.5 3,420,317 + +0.6 3,420,318 - -0.1 3,420,318 - -1.1 3,420,318 - +0.6 3,420,318 - +0.2 3,420,378 + +1.8 3,420,378 + -1.1 3,420,378 + -0.6 3,420,391 + +0.7 3,420,392 - +0.0 3,420,484 + -0.6 3,420,484 + +0.6 3,420,485 - -0.2 3,420,487 - -0.4 3,420,499 + +0.6 3,420,500 - -1.1 3,420,500 - -0.6 3,420,556 + PfGW456L13_3073 0.14 -0.4 3,420,556 + PfGW456L13_3073 0.14 -0.5 3,420,557 - PfGW456L13_3073 0.14 +0.4 3,420,557 - PfGW456L13_3073 0.14 +0.1 3,420,557 - PfGW456L13_3073 0.14 +1.7 3,420,557 - PfGW456L13_3073 0.14 +0.6 3,420,557 - PfGW456L13_3073 0.14 +0.6 3,420,557 - PfGW456L13_3073 0.14 -0.3 3,420,557 - PfGW456L13_3073 0.14 +0.8 3,420,557 - PfGW456L13_3073 0.14 -0.9 3,420,566 - PfGW456L13_3073 0.15 +0.8 3,420,566 - PfGW456L13_3073 0.15 -0.6 3,420,779 - PfGW456L13_3073 0.34 +0.2 3,420,806 - PfGW456L13_3073 0.37 +1.2 3,420,806 - PfGW456L13_3073 0.37 -1.2 3,420,959 - PfGW456L13_3073 0.51 -0.4 3,420,997 + PfGW456L13_3073 0.54 +0.8 3,420,997 + +1.2 3,420,998 - PfGW456L13_3073 0.55 +0.9 3,420,998 - PfGW456L13_3073 0.55 +0.8 3,421,036 + PfGW456L13_3073 0.58 +0.8 3,421,037 - PfGW456L13_3073 0.58 -0.2 3,421,037 - +2.9 3,421,037 - PfGW456L13_3073 0.58 -0.5 3,421,037 - PfGW456L13_3073 0.58 -1.0 3,421,045 + PfGW456L13_3073 0.59 -0.5 3,421,046 - PfGW456L13_3073 0.59 +0.1 3,421,046 - PfGW456L13_3073 0.59 +0.1 3,421,046 - PfGW456L13_3073 0.59 -0.5 3,421,046 - PfGW456L13_3073 0.59 -1.1 3,421,063 + PfGW456L13_3073 0.60 -0.1 3,421,064 - PfGW456L13_3073 0.61 +1.2 3,421,064 - PfGW456L13_3073 0.61 +0.6 3,421,064 - PfGW456L13_3073 0.61 -1.1 3,421,064 - PfGW456L13_3073 0.61 -0.0 3,421,064 - PfGW456L13_3073 0.61 +0.9 3,421,064 - PfGW456L13_3073 0.61 +1.8 3,421,263 + PfGW456L13_3073 0.79 +0.0 3,421,263 + PfGW456L13_3073 0.79 +0.3 3,421,264 - PfGW456L13_3073 0.79 +0.6 3,421,270 + PfGW456L13_3073 0.80 -0.4 3,421,271 - PfGW456L13_3073 0.80 +0.9 3,421,309 + PfGW456L13_3073 0.83 +0.1 3,421,309 + PfGW456L13_3073 0.83 +0.5 3,421,309 + PfGW456L13_3073 0.83 +1.0 3,421,309 + PfGW456L13_3073 0.83 +1.2 3,421,310 - PfGW456L13_3073 0.83 +0.8 3,421,310 - PfGW456L13_3073 0.83 +0.1 3,421,310 - PfGW456L13_3073 0.83 -0.2 3,421,310 - PfGW456L13_3073 0.83 -1.0 3,421,310 - PfGW456L13_3073 0.83 +0.1 3,421,310 - PfGW456L13_3073 0.83 -0.2
Or see this region's nucleotide sequence