Experiment: Fusidic 0.03 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_2530 and PfGW456L13_2531 are separated by 11 nucleotides PfGW456L13_2531 and PfGW456L13_2532 are separated by 12 nucleotides PfGW456L13_2532 and PfGW456L13_2533 are separated by 23 nucleotides PfGW456L13_2533 and PfGW456L13_2534 are separated by 2 nucleotides
PfGW456L13_2530 - 2,3-dihydroxybiphenyl 1,2-dioxygenase, at 2,798,222 to 2,799,118
_2530
PfGW456L13_2531 - 3-oxoadipate CoA-transferase subunit A (EC 2.8.3.6); Glutaconate CoA-transferase subunit A (EC 2.8.3.12), at 2,799,130 to 2,800,011
_2531
PfGW456L13_2532 - 3-oxoadipate CoA-transferase subunit B (EC 2.8.3.6); Glutaconate CoA-transferase subunit B (EC 2.8.3.12), at 2,800,024 to 2,800,812
_2532
PfGW456L13_2533 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,800,836 to 2,801,708
_2533
PfGW456L13_2534 - Enoyl-[acyl-carrier-protein] reductase [FMN] (EC 1.3.1.9), at 2,801,711 to 2,802,778
_2534
Position (kb)
2800
2801 Strain fitness (log2 ratio)
-1
0
1 at 2799.360 kb on + strand, within PfGW456L13_2531 at 2799.361 kb on - strand, within PfGW456L13_2531 at 2799.376 kb on + strand, within PfGW456L13_2531 at 2799.376 kb on + strand, within PfGW456L13_2531 at 2799.376 kb on + strand, within PfGW456L13_2531 at 2799.376 kb on + strand, within PfGW456L13_2531 at 2799.376 kb on + strand, within PfGW456L13_2531 at 2799.376 kb on + strand, within PfGW456L13_2531 at 2799.376 kb on + strand, within PfGW456L13_2531 at 2799.377 kb on - strand, within PfGW456L13_2531 at 2799.377 kb on - strand, within PfGW456L13_2531 at 2799.551 kb on - strand, within PfGW456L13_2531 at 2799.664 kb on + strand, within PfGW456L13_2531 at 2799.664 kb on + strand, within PfGW456L13_2531 at 2799.665 kb on - strand, within PfGW456L13_2531 at 2799.703 kb on + strand, within PfGW456L13_2531 at 2799.704 kb on - strand, within PfGW456L13_2531 at 2799.704 kb on - strand, within PfGW456L13_2531 at 2799.704 kb on - strand, within PfGW456L13_2531 at 2799.704 kb on - strand, within PfGW456L13_2531 at 2799.704 kb on - strand, within PfGW456L13_2531 at 2799.737 kb on - strand, within PfGW456L13_2531 at 2799.847 kb on + strand, within PfGW456L13_2531 at 2799.877 kb on + strand, within PfGW456L13_2531 at 2799.878 kb on - strand, within PfGW456L13_2531 at 2799.917 kb on - strand, within PfGW456L13_2531 at 2799.917 kb on - strand, within PfGW456L13_2531 at 2799.929 kb on - strand at 2799.952 kb on + strand at 2799.952 kb on + strand at 2800.010 kb on - strand at 2800.032 kb on + strand at 2800.032 kb on + strand at 2800.033 kb on - strand at 2800.039 kb on + strand at 2800.040 kb on - strand at 2800.100 kb on + strand at 2800.100 kb on + strand at 2800.101 kb on - strand at 2800.195 kb on + strand, within PfGW456L13_2532 at 2800.195 kb on + strand, within PfGW456L13_2532 at 2800.195 kb on + strand, within PfGW456L13_2532 at 2800.195 kb on + strand, within PfGW456L13_2532 at 2800.195 kb on + strand, within PfGW456L13_2532 at 2800.196 kb on - strand, within PfGW456L13_2532 at 2800.196 kb on - strand, within PfGW456L13_2532 at 2800.196 kb on - strand, within PfGW456L13_2532 at 2800.514 kb on - strand, within PfGW456L13_2532 at 2800.879 kb on - strand at 2800.956 kb on + strand, within PfGW456L13_2533 at 2800.957 kb on - strand at 2800.957 kb on - strand, within PfGW456L13_2533 at 2800.957 kb on - strand, within PfGW456L13_2533 at 2800.957 kb on - strand, within PfGW456L13_2533 at 2801.146 kb on - strand, within PfGW456L13_2533 at 2801.166 kb on + strand, within PfGW456L13_2533 at 2801.166 kb on + strand, within PfGW456L13_2533 at 2801.166 kb on + strand, within PfGW456L13_2533 at 2801.167 kb on - strand, within PfGW456L13_2533 at 2801.295 kb on + strand, within PfGW456L13_2533 at 2801.295 kb on + strand, within PfGW456L13_2533 at 2801.295 kb on + strand, within PfGW456L13_2533 at 2801.295 kb on + strand, within PfGW456L13_2533 at 2801.295 kb on + strand, within PfGW456L13_2533 at 2801.296 kb on - strand, within PfGW456L13_2533 at 2801.296 kb on - strand, within PfGW456L13_2533 at 2801.296 kb on - strand, within PfGW456L13_2533 at 2801.296 kb on - strand, within PfGW456L13_2533 at 2801.296 kb on - strand, within PfGW456L13_2533 at 2801.296 kb on - strand, within PfGW456L13_2533 at 2801.296 kb on - strand, within PfGW456L13_2533 at 2801.296 kb on - strand, within PfGW456L13_2533 at 2801.296 kb on - strand, within PfGW456L13_2533 at 2801.296 kb on - strand, within PfGW456L13_2533 at 2801.296 kb on - strand, within PfGW456L13_2533 at 2801.340 kb on + strand, within PfGW456L13_2533 at 2801.340 kb on + strand, within PfGW456L13_2533 at 2801.691 kb on - strand at 2801.691 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.03 mg/ml remove 2,799,360 + PfGW456L13_2531 0.26 +1.2 2,799,361 - PfGW456L13_2531 0.26 +0.6 2,799,376 + PfGW456L13_2531 0.28 +1.2 2,799,376 + PfGW456L13_2531 0.28 +0.3 2,799,376 + PfGW456L13_2531 0.28 +0.7 2,799,376 + PfGW456L13_2531 0.28 -0.9 2,799,376 + PfGW456L13_2531 0.28 +0.0 2,799,376 + PfGW456L13_2531 0.28 +1.0 2,799,376 + PfGW456L13_2531 0.28 +0.7 2,799,377 - PfGW456L13_2531 0.28 -0.5 2,799,377 - PfGW456L13_2531 0.28 -0.4 2,799,551 - PfGW456L13_2531 0.48 +0.0 2,799,664 + PfGW456L13_2531 0.61 -0.2 2,799,664 + PfGW456L13_2531 0.61 +0.2 2,799,665 - PfGW456L13_2531 0.61 -0.2 2,799,703 + PfGW456L13_2531 0.65 -0.1 2,799,704 - PfGW456L13_2531 0.65 -0.4 2,799,704 - PfGW456L13_2531 0.65 -0.9 2,799,704 - PfGW456L13_2531 0.65 -1.2 2,799,704 - PfGW456L13_2531 0.65 +0.5 2,799,704 - PfGW456L13_2531 0.65 +1.5 2,799,737 - PfGW456L13_2531 0.69 -0.0 2,799,847 + PfGW456L13_2531 0.81 +0.0 2,799,877 + PfGW456L13_2531 0.85 +0.0 2,799,878 - PfGW456L13_2531 0.85 +1.0 2,799,917 - PfGW456L13_2531 0.89 -0.5 2,799,917 - PfGW456L13_2531 0.89 -0.2 2,799,929 - +0.7 2,799,952 + +0.1 2,799,952 + +1.5 2,800,010 - +0.4 2,800,032 + -1.2 2,800,032 + -0.7 2,800,033 - -0.3 2,800,039 + -0.5 2,800,040 - -0.7 2,800,100 + -0.8 2,800,100 + -1.1 2,800,101 - -0.0 2,800,195 + PfGW456L13_2532 0.22 -1.1 2,800,195 + PfGW456L13_2532 0.22 -0.0 2,800,195 + PfGW456L13_2532 0.22 -0.3 2,800,195 + PfGW456L13_2532 0.22 -0.3 2,800,195 + PfGW456L13_2532 0.22 +0.8 2,800,196 - PfGW456L13_2532 0.22 +0.2 2,800,196 - PfGW456L13_2532 0.22 +0.2 2,800,196 - PfGW456L13_2532 0.22 +1.0 2,800,514 - PfGW456L13_2532 0.62 -1.5 2,800,879 - -1.4 2,800,956 + PfGW456L13_2533 0.14 +0.4 2,800,957 - -0.2 2,800,957 - PfGW456L13_2533 0.14 +0.2 2,800,957 - PfGW456L13_2533 0.14 -0.4 2,800,957 - PfGW456L13_2533 0.14 -1.2 2,801,146 - PfGW456L13_2533 0.36 -0.2 2,801,166 + PfGW456L13_2533 0.38 +0.4 2,801,166 + PfGW456L13_2533 0.38 +0.8 2,801,166 + PfGW456L13_2533 0.38 +0.1 2,801,167 - PfGW456L13_2533 0.38 +0.2 2,801,295 + PfGW456L13_2533 0.53 +0.2 2,801,295 + PfGW456L13_2533 0.53 -0.8 2,801,295 + PfGW456L13_2533 0.53 -0.1 2,801,295 + PfGW456L13_2533 0.53 +1.0 2,801,295 + PfGW456L13_2533 0.53 -0.1 2,801,296 - PfGW456L13_2533 0.53 +0.3 2,801,296 - PfGW456L13_2533 0.53 -0.4 2,801,296 - PfGW456L13_2533 0.53 -1.2 2,801,296 - PfGW456L13_2533 0.53 -0.5 2,801,296 - PfGW456L13_2533 0.53 -0.5 2,801,296 - PfGW456L13_2533 0.53 +0.0 2,801,296 - PfGW456L13_2533 0.53 -0.3 2,801,296 - PfGW456L13_2533 0.53 +0.0 2,801,296 - PfGW456L13_2533 0.53 +0.3 2,801,296 - PfGW456L13_2533 0.53 +0.6 2,801,296 - PfGW456L13_2533 0.53 -0.0 2,801,340 + PfGW456L13_2533 0.58 +0.0 2,801,340 + PfGW456L13_2533 0.58 +0.8 2,801,691 - +0.7 2,801,691 - +0.5
Or see this region's nucleotide sequence