Experiment: Fusidic 0.03 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_138 and PfGW456L13_139 are separated by 122 nucleotides PfGW456L13_139 and PfGW456L13_140 are separated by 19 nucleotides PfGW456L13_140 and PfGW456L13_141 are separated by 36 nucleotides PfGW456L13_141 and PfGW456L13_142 are separated by 28 nucleotides
PfGW456L13_138 - Replicative DNA helicase (EC 3.6.1.-), at 159,395 to 160,792
_138
PfGW456L13_139 - LSU ribosomal protein L9p, at 160,915 to 161,361
_139
PfGW456L13_140 - FIG003573: hypothetical protein, at 161,381 to 162,274
_140
PfGW456L13_141 - SSU ribosomal protein S18p @ SSU ribosomal protein S18p, zinc-independent, at 162,311 to 162,541
_141
PfGW456L13_142 - SSU ribosomal protein S6p, at 162,570 to 162,992
_142
Position (kb) 161
162
163 Strain fitness (log2 ratio) -2
-1
0
1 at 160.814 kb on - strand at 160.814 kb on - strand at 160.814 kb on - strand at 160.814 kb on - strand at 160.991 kb on - strand, within PfGW456L13_139 at 161.376 kb on - strand at 161.479 kb on - strand, within PfGW456L13_140 at 161.479 kb on - strand, within PfGW456L13_140 at 161.479 kb on - strand, within PfGW456L13_140 at 161.479 kb on - strand, within PfGW456L13_140 at 161.512 kb on - strand, within PfGW456L13_140 at 161.512 kb on - strand, within PfGW456L13_140 at 161.512 kb on - strand, within PfGW456L13_140 at 161.512 kb on - strand, within PfGW456L13_140 at 163.142 kb on + strand at 163.143 kb on - strand at 163.154 kb on - strand at 163.182 kb on + strand at 163.183 kb on - strand at 163.237 kb on + strand at 163.237 kb on + strand at 163.237 kb on + strand at 163.238 kb on - strand at 163.238 kb on - strand at 163.250 kb on + strand at 163.250 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.03 mg/ml remove 160,814 - -0.5 160,814 - -1.5 160,814 - -0.5 160,814 - +0.4 160,991 - PfGW456L13_139 0.17 -0.5 161,376 - -2.2 161,479 - PfGW456L13_140 0.11 -1.3 161,479 - PfGW456L13_140 0.11 -1.1 161,479 - PfGW456L13_140 0.11 +0.1 161,479 - PfGW456L13_140 0.11 -0.5 161,512 - PfGW456L13_140 0.15 -1.3 161,512 - PfGW456L13_140 0.15 -1.9 161,512 - PfGW456L13_140 0.15 +0.8 161,512 - PfGW456L13_140 0.15 -1.0 163,142 + +0.3 163,143 - +0.3 163,154 - +0.0 163,182 + +1.6 163,183 - +0.2 163,237 + +0.5 163,237 + -0.2 163,237 + -0.1 163,238 - +1.1 163,238 - -0.1 163,250 + +0.4 163,250 + -0.7
Or see this region's nucleotide sequence