Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF895 and GFF896 are separated by 194 nucleotides GFF896 and GFF897 are separated by 29 nucleotides GFF897 and GFF898 overlap by 29 nucleotides
GFF895 - 5-keto-2-deoxygluconokinase (EC 2.7.1.92) / uncharacterized domain, at 75,766 to 77,697
GFF895
GFF896 - hypothetical protein, at 77,892 to 78,005
GFF896
GFF897 - hypothetical protein, at 78,035 to 78,169
GFF897
GFF898 - Epi-inositol hydrolase (EC 3.7.1.-), at 78,141 to 80,060
GFF898
Position (kb) 78
79 Strain fitness (log2 ratio) -1
0
1 at 77.077 kb on + strand, within GFF895 at 77.077 kb on + strand, within GFF895 at 77.078 kb on - strand, within GFF895 at 77.137 kb on + strand, within GFF895 at 77.210 kb on + strand, within GFF895 at 77.211 kb on - strand, within GFF895 at 77.211 kb on - strand, within GFF895 at 77.351 kb on + strand, within GFF895 at 77.521 kb on + strand at 77.574 kb on - strand at 77.593 kb on - strand at 77.593 kb on - strand at 77.625 kb on - strand at 77.703 kb on + strand at 78.035 kb on - strand at 78.073 kb on + strand, within GFF897 at 78.073 kb on + strand, within GFF897 at 78.074 kb on - strand, within GFF897 at 78.074 kb on - strand, within GFF897 at 78.118 kb on - strand, within GFF897 at 78.118 kb on - strand, within GFF897 at 78.177 kb on + strand at 78.410 kb on + strand, within GFF898 at 78.478 kb on - strand, within GFF898 at 78.605 kb on - strand, within GFF898 at 78.618 kb on - strand, within GFF898 at 79.089 kb on + strand, within GFF898 at 79.090 kb on - strand, within GFF898 at 79.094 kb on - strand, within GFF898
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 77,077 + GFF895 0.68 -0.4 77,077 + GFF895 0.68 +0.8 77,078 - GFF895 0.68 -0.2 77,137 + GFF895 0.71 +0.4 77,210 + GFF895 0.75 +0.4 77,211 - GFF895 0.75 -0.3 77,211 - GFF895 0.75 -0.5 77,351 + GFF895 0.82 -0.2 77,521 + -0.5 77,574 - +0.1 77,593 - -1.3 77,593 - +1.1 77,625 - -0.4 77,703 + -0.7 78,035 - -0.3 78,073 + GFF897 0.28 -0.9 78,073 + GFF897 0.28 -1.3 78,074 - GFF897 0.29 +0.4 78,074 - GFF897 0.29 -0.5 78,118 - GFF897 0.61 -0.9 78,118 - GFF897 0.61 -0.4 78,177 + -0.3 78,410 + GFF898 0.14 -0.4 78,478 - GFF898 0.18 -0.3 78,605 - GFF898 0.24 -0.5 78,618 - GFF898 0.25 -0.1 79,089 + GFF898 0.49 -0.8 79,090 - GFF898 0.49 -0.7 79,094 - GFF898 0.50 +0.0
Or see this region's nucleotide sequence