Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF871 and GFF872 are separated by 182 nucleotides GFF872 and GFF873 are separated by 54 nucleotides GFF873 and GFF874 are separated by 41 nucleotides
GFF871 - Putative membrane protein, at 47,025 to 48,368
GFF871
GFF872 - Peptide methionine sulfoxide reductase MsrA (EC 1.8.4.11), at 48,551 to 49,189
GFF872
GFF873 - hypothetical protein, at 49,244 to 49,360
GFF873
GFF874 - Uncharacterized protein YtfM precursor, at 49,402 to 51,135
GFF874
Position (kb)
49
50 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 48.246 kb on - strand at 48.264 kb on + strand at 48.264 kb on + strand at 48.265 kb on - strand at 48.265 kb on - strand at 48.269 kb on + strand at 48.270 kb on - strand at 48.281 kb on + strand at 48.345 kb on + strand at 48.346 kb on - strand at 48.355 kb on - strand at 48.409 kb on + strand at 48.410 kb on - strand at 48.412 kb on - strand at 48.474 kb on + strand at 48.484 kb on + strand at 48.485 kb on - strand at 48.485 kb on - strand at 48.485 kb on - strand at 48.490 kb on - strand at 48.494 kb on + strand at 48.538 kb on + strand at 48.649 kb on - strand, within GFF872 at 48.806 kb on - strand, within GFF872 at 48.888 kb on + strand, within GFF872 at 48.888 kb on + strand, within GFF872 at 48.889 kb on - strand, within GFF872 at 48.988 kb on + strand, within GFF872 at 48.988 kb on + strand, within GFF872 at 48.989 kb on - strand, within GFF872 at 48.989 kb on - strand, within GFF872 at 48.992 kb on + strand, within GFF872 at 48.992 kb on + strand, within GFF872 at 49.051 kb on - strand, within GFF872 at 49.085 kb on - strand, within GFF872 at 49.212 kb on - strand at 49.285 kb on - strand, within GFF873 at 49.421 kb on + strand at 49.626 kb on - strand, within GFF874 at 49.680 kb on - strand, within GFF874 at 49.769 kb on - strand, within GFF874 at 49.769 kb on - strand, within GFF874 at 49.778 kb on + strand, within GFF874 at 49.893 kb on + strand, within GFF874 at 49.948 kb on + strand, within GFF874 at 50.015 kb on - strand, within GFF874 at 50.109 kb on + strand, within GFF874 at 50.281 kb on + strand, within GFF874 at 50.347 kb on - strand, within GFF874
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 48,246 - +1.0 48,264 + -0.3 48,264 + -0.3 48,265 - +0.3 48,265 - -0.3 48,269 + -1.8 48,270 - +0.8 48,281 + -0.0 48,345 + -0.0 48,346 - -0.8 48,355 - -0.4 48,409 + +0.2 48,410 - +0.0 48,412 - +0.4 48,474 + -0.7 48,484 + -0.7 48,485 - -1.4 48,485 - -0.8 48,485 - +1.9 48,490 - -0.5 48,494 + -0.7 48,538 + +0.1 48,649 - GFF872 0.15 -0.4 48,806 - GFF872 0.40 -2.0 48,888 + GFF872 0.53 -0.1 48,888 + GFF872 0.53 -1.1 48,889 - GFF872 0.53 +0.3 48,988 + GFF872 0.68 -0.6 48,988 + GFF872 0.68 +0.2 48,989 - GFF872 0.69 +0.4 48,989 - GFF872 0.69 +0.2 48,992 + GFF872 0.69 -0.1 48,992 + GFF872 0.69 -2.0 49,051 - GFF872 0.78 -0.4 49,085 - GFF872 0.84 +0.2 49,212 - +0.2 49,285 - GFF873 0.35 -0.9 49,421 + +1.1 49,626 - GFF874 0.13 +1.7 49,680 - GFF874 0.16 -0.3 49,769 - GFF874 0.21 +0.9 49,769 - GFF874 0.21 -0.9 49,778 + GFF874 0.22 -0.5 49,893 + GFF874 0.28 +1.9 49,948 + GFF874 0.31 -0.8 50,015 - GFF874 0.35 +0.9 50,109 + GFF874 0.41 +0.8 50,281 + GFF874 0.51 +0.6 50,347 - GFF874 0.54 +0.5
Or see this region's nucleotide sequence