Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF793 and GFF794 are separated by 504 nucleotides GFF794 and GFF795 are separated by 2 nucleotides GFF795 and GFF796 overlap by 4 nucleotides
GFF793 - hypothetical protein, at 143,258 to 143,371
GFF793
GFF794 - Phage holin, at 143,876 to 144,220
GFF794
GFF795 - Phage lysin, 1,4-beta-N-acetylmuramidase (EC 3.2.1.17) or lysozyme, at 144,223 to 144,837
GFF795
GFF796 - Phage outer membrane lytic protein Rz; Endopeptidase (EC 3.4.-.-), at 144,834 to 145,319
GFF796
Position (kb)
143
144
145 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 143.281 kb on - strand, within GFF793 at 143.431 kb on - strand at 143.481 kb on - strand at 143.518 kb on + strand at 143.518 kb on + strand at 143.519 kb on - strand at 143.589 kb on + strand at 143.631 kb on - strand at 143.683 kb on + strand at 143.691 kb on + strand at 143.793 kb on + strand at 143.806 kb on - strand at 143.814 kb on - strand at 143.950 kb on - strand, within GFF794 at 144.003 kb on - strand, within GFF794 at 144.477 kb on + strand, within GFF795 at 144.477 kb on + strand, within GFF795 at 144.478 kb on - strand, within GFF795 at 144.588 kb on + strand, within GFF795 at 144.733 kb on + strand, within GFF795 at 144.850 kb on + strand at 144.850 kb on + strand at 145.001 kb on + strand, within GFF796 at 145.001 kb on + strand, within GFF796 at 145.001 kb on + strand, within GFF796 at 145.002 kb on - strand, within GFF796 at 145.051 kb on - strand, within GFF796
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 143,281 - GFF793 0.20 +0.9 143,431 - +0.0 143,481 - +2.3 143,518 + +0.3 143,518 + +0.3 143,519 - +0.2 143,589 + +1.0 143,631 - -0.0 143,683 + +0.4 143,691 + -0.1 143,793 + -0.2 143,806 - -1.6 143,814 - -0.4 143,950 - GFF794 0.21 -0.1 144,003 - GFF794 0.37 -1.1 144,477 + GFF795 0.41 -0.8 144,477 + GFF795 0.41 -0.4 144,478 - GFF795 0.41 +1.4 144,588 + GFF795 0.59 -1.2 144,733 + GFF795 0.83 +1.9 144,850 + -0.4 144,850 + +0.7 145,001 + GFF796 0.34 -0.7 145,001 + GFF796 0.34 -0.9 145,001 + GFF796 0.34 +1.0 145,002 - GFF796 0.35 +0.3 145,051 - GFF796 0.45 -2.7
Or see this region's nucleotide sequence