Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF671 and GFF672 overlap by 4 nucleotides GFF672 and GFF673 overlap by 17 nucleotides GFF673 and GFF674 overlap by 8 nucleotides
GFF671 - Biotin synthase (EC 2.8.1.6), at 27,234 to 28,274
GFF671
GFF672 - 8-amino-7-oxononanoate synthase (EC 2.3.1.47), at 28,271 to 29,428
GFF672
GFF673 - Biotin synthesis protein BioC, at 29,412 to 30,167
GFF673
GFF674 - Dethiobiotin synthetase (EC 6.3.3.3), at 30,160 to 30,846
GFF674
Position (kb) 28
29
30 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 27.353 kb on - strand, within GFF671 at 27.362 kb on + strand, within GFF671 at 27.363 kb on - strand, within GFF671 at 27.404 kb on + strand, within GFF671 at 27.405 kb on - strand, within GFF671 at 27.467 kb on - strand, within GFF671 at 27.476 kb on - strand, within GFF671 at 27.681 kb on + strand, within GFF671 at 27.681 kb on + strand, within GFF671 at 27.723 kb on - strand, within GFF671 at 27.738 kb on - strand, within GFF671 at 27.741 kb on + strand, within GFF671 at 27.741 kb on + strand, within GFF671 at 27.742 kb on - strand, within GFF671 at 27.788 kb on + strand, within GFF671 at 27.789 kb on - strand, within GFF671 at 27.789 kb on - strand, within GFF671 at 28.005 kb on + strand, within GFF671 at 28.005 kb on + strand, within GFF671 at 28.151 kb on + strand, within GFF671 at 28.254 kb on + strand at 28.255 kb on - strand at 28.324 kb on + strand at 28.341 kb on - strand at 28.341 kb on - strand at 28.391 kb on + strand, within GFF672 at 28.391 kb on + strand, within GFF672 at 28.392 kb on - strand, within GFF672 at 28.397 kb on - strand, within GFF672 at 28.425 kb on + strand, within GFF672 at 28.585 kb on + strand, within GFF672 at 28.802 kb on + strand, within GFF672 at 28.802 kb on + strand, within GFF672 at 28.802 kb on + strand, within GFF672 at 28.802 kb on + strand, within GFF672 at 28.803 kb on - strand, within GFF672 at 28.803 kb on - strand, within GFF672 at 28.803 kb on - strand, within GFF672 at 29.426 kb on + strand at 29.426 kb on + strand at 29.577 kb on - strand, within GFF673 at 29.577 kb on - strand, within GFF673 at 29.577 kb on - strand, within GFF673 at 29.790 kb on + strand, within GFF673 at 29.791 kb on - strand, within GFF673 at 30.080 kb on + strand, within GFF673 at 30.197 kb on - strand at 30.249 kb on - strand, within GFF674 at 30.249 kb on - strand, within GFF674 at 30.264 kb on - strand, within GFF674 at 30.264 kb on - strand, within GFF674 at 30.268 kb on + strand, within GFF674 at 30.268 kb on + strand, within GFF674 at 30.271 kb on - strand, within GFF674 at 30.384 kb on + strand, within GFF674 at 30.399 kb on + strand, within GFF674 at 30.399 kb on + strand, within GFF674
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 27,353 - GFF671 0.11 -0.9 27,362 + GFF671 0.12 +1.2 27,363 - GFF671 0.12 +0.2 27,404 + GFF671 0.16 -0.2 27,405 - GFF671 0.16 -0.9 27,467 - GFF671 0.22 +0.3 27,476 - GFF671 0.23 -0.5 27,681 + GFF671 0.43 +0.5 27,681 + GFF671 0.43 -0.0 27,723 - GFF671 0.47 +0.7 27,738 - GFF671 0.48 +0.3 27,741 + GFF671 0.49 -0.3 27,741 + GFF671 0.49 +0.4 27,742 - GFF671 0.49 -0.1 27,788 + GFF671 0.53 +0.3 27,789 - GFF671 0.53 +3.0 27,789 - GFF671 0.53 -1.1 28,005 + GFF671 0.74 +0.7 28,005 + GFF671 0.74 -0.9 28,151 + GFF671 0.88 -0.1 28,254 + +0.0 28,255 - -0.3 28,324 + -0.1 28,341 - +2.3 28,341 - -0.1 28,391 + GFF672 0.10 -0.1 28,391 + GFF672 0.10 -1.8 28,392 - GFF672 0.10 +0.9 28,397 - GFF672 0.11 +0.1 28,425 + GFF672 0.13 +0.3 28,585 + GFF672 0.27 -1.2 28,802 + GFF672 0.46 -0.2 28,802 + GFF672 0.46 -0.4 28,802 + GFF672 0.46 -0.7 28,802 + GFF672 0.46 -0.4 28,803 - GFF672 0.46 +0.2 28,803 - GFF672 0.46 -0.4 28,803 - GFF672 0.46 +0.3 29,426 + -0.0 29,426 + -0.3 29,577 - GFF673 0.22 +0.2 29,577 - GFF673 0.22 -0.8 29,577 - GFF673 0.22 -0.6 29,790 + GFF673 0.50 +0.8 29,791 - GFF673 0.50 +0.3 30,080 + GFF673 0.88 -0.6 30,197 - +0.5 30,249 - GFF674 0.13 +1.0 30,249 - GFF674 0.13 -0.2 30,264 - GFF674 0.15 -0.9 30,264 - GFF674 0.15 +1.3 30,268 + GFF674 0.16 +0.1 30,268 + GFF674 0.16 +0.7 30,271 - GFF674 0.16 +0.9 30,384 + GFF674 0.33 -1.4 30,399 + GFF674 0.35 +2.2 30,399 + GFF674 0.35 -0.5
Or see this region's nucleotide sequence