Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF655 and GFF656 are separated by 128 nucleotides GFF656 and GFF657 are separated by 166 nucleotides GFF657 and GFF658 overlap by 1 nucleotides GFF658 and GFF659 are separated by 2 nucleotides
GFF655 - DNA-binding domain of ModE / Molybdate-binding domain of ModE, at 11,506 to 12,294
GFF655
GFF656 - Putative exported protein, at 12,423 to 12,572
GFF656
GFF657 - Molybdenum ABC transporter, periplasmic molybdenum-binding protein ModA (TC 3.A.1.8.1), at 12,739 to 13,512
GFF657
GFF658 - Molybdenum transport system permease protein ModB (TC 3.A.1.8.1), at 13,512 to 14,201
GFF658
GFF659 - Molybdenum transport ATP-binding protein ModC (TC 3.A.1.8.1), at 14,204 to 15,262
GFF659
Position (kb) 12
13
14 Strain fitness (log2 ratio) -1
0
1
2 at 12.029 kb on + strand, within GFF655 at 12.111 kb on + strand, within GFF655 at 12.111 kb on + strand, within GFF655 at 12.112 kb on - strand, within GFF655 at 12.112 kb on - strand, within GFF655 at 12.159 kb on + strand, within GFF655 at 12.160 kb on - strand, within GFF655 at 12.162 kb on - strand, within GFF655 at 12.231 kb on + strand at 12.232 kb on - strand at 12.394 kb on + strand at 12.407 kb on - strand at 12.409 kb on - strand at 12.649 kb on - strand at 12.735 kb on - strand at 12.831 kb on - strand, within GFF657 at 12.947 kb on + strand, within GFF657 at 13.104 kb on + strand, within GFF657 at 13.317 kb on - strand, within GFF657 at 13.448 kb on - strand at 13.516 kb on + strand at 13.584 kb on - strand, within GFF658 at 13.615 kb on + strand, within GFF658 at 13.648 kb on + strand, within GFF658 at 13.702 kb on - strand, within GFF658 at 13.958 kb on + strand, within GFF658 at 14.054 kb on - strand, within GFF658 at 14.054 kb on - strand, within GFF658 at 14.067 kb on + strand, within GFF658 at 14.067 kb on + strand, within GFF658 at 14.068 kb on - strand, within GFF658 at 14.068 kb on - strand, within GFF658 at 14.070 kb on - strand, within GFF658 at 14.131 kb on + strand, within GFF658 at 14.131 kb on + strand, within GFF658 at 14.132 kb on - strand, within GFF658 at 14.248 kb on - strand at 14.475 kb on - strand, within GFF659
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 12,029 + GFF655 0.66 +0.3 12,111 + GFF655 0.77 -0.6 12,111 + GFF655 0.77 -0.4 12,112 - GFF655 0.77 -0.7 12,112 - GFF655 0.77 +2.5 12,159 + GFF655 0.83 -0.3 12,160 - GFF655 0.83 +0.5 12,162 - GFF655 0.83 +0.3 12,231 + +0.2 12,232 - +1.2 12,394 + +0.5 12,407 - +0.1 12,409 - +0.4 12,649 - +0.3 12,735 - +0.4 12,831 - GFF657 0.12 -0.2 12,947 + GFF657 0.27 -1.4 13,104 + GFF657 0.47 -0.8 13,317 - GFF657 0.75 -0.9 13,448 - -0.7 13,516 + -0.3 13,584 - GFF658 0.10 -0.5 13,615 + GFF658 0.15 -0.3 13,648 + GFF658 0.20 +0.9 13,702 - GFF658 0.28 -1.2 13,958 + GFF658 0.65 +0.3 14,054 - GFF658 0.79 +1.3 14,054 - GFF658 0.79 -0.0 14,067 + GFF658 0.80 -0.3 14,067 + GFF658 0.80 -0.9 14,068 - GFF658 0.81 -0.2 14,068 - GFF658 0.81 -0.4 14,070 - GFF658 0.81 +0.1 14,131 + GFF658 0.90 -0.2 14,131 + GFF658 0.90 +0.6 14,132 - GFF658 0.90 +1.0 14,248 - +2.0 14,475 - GFF659 0.26 +1.0
Or see this region's nucleotide sequence