Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4773 and GFF4774 are separated by 55 nucleotides GFF4774 and GFF4775 overlap by 4 nucleotides GFF4775 and GFF4776 are separated by 108 nucleotides
GFF4773 - DNA mismatch repair protein MutS, at 80,062 to 82,629
GFF4773
GFF4774 - FIG074102: hypothetical protein, at 82,685 to 83,065
GFF4774
GFF4775 - Possible membrane transport protein, at 83,062 to 84,270
GFF4775
GFF4776 - LysR-family transcriptional regulator STM2912, at 84,379 to 85,290
GFF4776
Position (kb) 83
84
85 Strain fitness (log2 ratio) -2
-1
0
1
2 at 82.113 kb on + strand, within GFF4773 at 82.171 kb on + strand, within GFF4773 at 82.171 kb on + strand, within GFF4773 at 82.225 kb on - strand, within GFF4773 at 82.253 kb on - strand, within GFF4773 at 82.330 kb on - strand, within GFF4773 at 83.008 kb on + strand, within GFF4774 at 83.055 kb on + strand at 83.120 kb on + strand at 83.121 kb on - strand at 83.157 kb on + strand at 83.158 kb on - strand at 83.305 kb on - strand, within GFF4775 at 83.305 kb on - strand, within GFF4775 at 83.416 kb on + strand, within GFF4775 at 83.426 kb on - strand, within GFF4775 at 83.589 kb on - strand, within GFF4775 at 83.623 kb on - strand, within GFF4775 at 83.941 kb on + strand, within GFF4775 at 83.967 kb on + strand, within GFF4775 at 83.990 kb on - strand, within GFF4775 at 84.121 kb on - strand, within GFF4775 at 84.123 kb on - strand, within GFF4775 at 84.159 kb on + strand at 84.159 kb on + strand at 84.159 kb on + strand at 84.159 kb on + strand at 84.160 kb on - strand at 84.239 kb on + strand at 84.263 kb on + strand at 84.263 kb on + strand at 84.272 kb on - strand at 84.321 kb on + strand at 84.341 kb on - strand at 84.342 kb on + strand at 84.343 kb on - strand at 84.343 kb on - strand at 84.343 kb on - strand at 84.397 kb on - strand at 84.420 kb on - strand at 84.521 kb on + strand, within GFF4776 at 84.527 kb on + strand, within GFF4776 at 84.664 kb on - strand, within GFF4776 at 84.959 kb on + strand, within GFF4776 at 84.990 kb on + strand, within GFF4776 at 85.020 kb on - strand, within GFF4776 at 85.214 kb on + strand at 85.214 kb on + strand at 85.215 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 82,113 + GFF4773 0.80 -1.0 82,171 + GFF4773 0.82 +1.4 82,171 + GFF4773 0.82 -0.4 82,225 - GFF4773 0.84 +0.8 82,253 - GFF4773 0.85 -0.1 82,330 - GFF4773 0.88 -0.5 83,008 + GFF4774 0.85 +0.8 83,055 + -0.2 83,120 + -1.8 83,121 - -0.7 83,157 + +0.1 83,158 - +0.2 83,305 - GFF4775 0.20 -0.3 83,305 - GFF4775 0.20 -0.3 83,416 + GFF4775 0.29 +0.2 83,426 - GFF4775 0.30 +0.1 83,589 - GFF4775 0.44 +0.8 83,623 - GFF4775 0.46 +0.5 83,941 + GFF4775 0.73 +0.2 83,967 + GFF4775 0.75 -0.2 83,990 - GFF4775 0.77 +1.0 84,121 - GFF4775 0.88 -0.5 84,123 - GFF4775 0.88 +0.7 84,159 + -0.1 84,159 + +0.0 84,159 + -0.0 84,159 + +0.7 84,160 - +0.0 84,239 + +0.7 84,263 + +0.5 84,263 + +0.4 84,272 - +0.0 84,321 + -0.3 84,341 - +0.2 84,342 + -0.5 84,343 - -0.2 84,343 - -0.5 84,343 - -0.7 84,397 - +0.2 84,420 - +0.6 84,521 + GFF4776 0.16 -0.9 84,527 + GFF4776 0.16 +1.1 84,664 - GFF4776 0.31 -0.2 84,959 + GFF4776 0.64 -0.2 84,990 + GFF4776 0.67 -0.0 85,020 - GFF4776 0.70 +1.8 85,214 + +0.8 85,214 + -0.1 85,215 - -0.4
Or see this region's nucleotide sequence