Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4411 and GFF4412 are separated by 154 nucleotides GFF4412 and GFF4413 overlap by 30 nucleotides GFF4413 and GFF4414 overlap by 4 nucleotides
GFF4411 - Putative NAD(P)-dependent oxidoreductase EC-YbbO, at 195,143 to 195,913
GFF4411
GFF4412 - Arylesterase precursor (EC 3.1.1.2), at 196,068 to 196,682
GFF4412
GFF4413 - Uncharacterized metabolite ABC transporter in Enterobacteriaceae, ATP-binding protein EC-YbbA, at 196,653 to 197,339
GFF4413
GFF4414 - FIG00809136: hypothetical protein, at 197,336 to 199,750
GFF4414
Position (kb) 196
197
198 Strain fitness (log2 ratio) -2
-1
0
1
2 at 195.654 kb on + strand, within GFF4411 at 195.655 kb on - strand, within GFF4411 at 195.687 kb on + strand, within GFF4411 at 195.960 kb on + strand at 195.961 kb on - strand at 195.961 kb on - strand at 195.972 kb on - strand at 195.998 kb on - strand at 196.011 kb on - strand at 196.011 kb on - strand at 196.011 kb on - strand at 196.014 kb on + strand at 196.014 kb on + strand at 196.015 kb on - strand at 196.018 kb on + strand at 196.018 kb on + strand at 196.019 kb on - strand at 196.019 kb on - strand at 196.019 kb on - strand at 196.019 kb on - strand at 196.038 kb on + strand at 196.112 kb on + strand at 196.176 kb on + strand, within GFF4412 at 196.176 kb on + strand, within GFF4412 at 196.176 kb on + strand, within GFF4412 at 196.229 kb on + strand, within GFF4412 at 196.259 kb on - strand, within GFF4412 at 196.345 kb on - strand, within GFF4412 at 196.380 kb on + strand, within GFF4412 at 196.472 kb on + strand, within GFF4412 at 196.482 kb on + strand, within GFF4412 at 196.526 kb on + strand, within GFF4412 at 196.527 kb on - strand, within GFF4412 at 196.593 kb on + strand, within GFF4412 at 196.950 kb on - strand, within GFF4413 at 196.950 kb on - strand, within GFF4413 at 197.324 kb on - strand at 197.340 kb on + strand at 197.341 kb on - strand at 197.341 kb on - strand at 197.485 kb on + strand at 197.552 kb on - strand at 197.640 kb on + strand, within GFF4414 at 197.641 kb on - strand, within GFF4414 at 197.645 kb on - strand, within GFF4414 at 197.645 kb on - strand, within GFF4414 at 197.867 kb on - strand, within GFF4414 at 198.205 kb on + strand, within GFF4414 at 198.208 kb on + strand, within GFF4414
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 195,654 + GFF4411 0.66 +2.3 195,655 - GFF4411 0.66 -0.7 195,687 + GFF4411 0.71 -1.5 195,960 + +0.9 195,961 - +0.4 195,961 - -0.2 195,972 - -0.2 195,998 - +0.5 196,011 - +1.1 196,011 - +0.3 196,011 - -0.2 196,014 + -0.0 196,014 + -1.8 196,015 - -0.5 196,018 + -1.2 196,018 + -0.8 196,019 - -0.6 196,019 - +0.4 196,019 - -0.4 196,019 - -0.1 196,038 + +0.6 196,112 + -0.9 196,176 + GFF4412 0.18 -0.2 196,176 + GFF4412 0.18 -0.8 196,176 + GFF4412 0.18 -0.2 196,229 + GFF4412 0.26 -0.2 196,259 - GFF4412 0.31 -1.1 196,345 - GFF4412 0.45 +0.1 196,380 + GFF4412 0.51 +1.0 196,472 + GFF4412 0.66 -0.6 196,482 + GFF4412 0.67 -1.1 196,526 + GFF4412 0.74 -0.6 196,527 - GFF4412 0.75 +0.0 196,593 + GFF4412 0.85 +0.4 196,950 - GFF4413 0.43 +1.2 196,950 - GFF4413 0.43 -0.6 197,324 - +0.2 197,340 + -2.6 197,341 - +1.6 197,341 - -2.4 197,485 + +0.2 197,552 - -0.3 197,640 + GFF4414 0.13 -0.4 197,641 - GFF4414 0.13 -0.6 197,645 - GFF4414 0.13 -0.7 197,645 - GFF4414 0.13 +0.0 197,867 - GFF4414 0.22 +0.4 198,205 + GFF4414 0.36 -0.7 198,208 + GFF4414 0.36 -0.1
Or see this region's nucleotide sequence