Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4328 and GFF4329 are separated by 2 nucleotides GFF4329 and GFF4330 are separated by 2 nucleotides GFF4330 and GFF4331 are separated by 5 nucleotides
GFF4328 - 2-aminoethylphosphonate ABC transporter permease protein II (TC 3.A.1.9.1), at 109,572 to 110,369
GFF4328
GFF4329 - 2-aminoethylphosphonate ABC transporter permease protein I (TC 3.A.1.9.1), at 110,372 to 111,232
GFF4329
GFF4330 - 2-aminoethylphosphonate ABC transporter ATP-binding protein (TC 3.A.1.9.1), at 111,235 to 112,344
GFF4330
GFF4331 - 2-aminoethylphosphonate ABC transporter periplasmic binding component (TC 3.A.1.9.1), at 112,350 to 113,363
GFF4331
Position (kb) 111
112
113 Strain fitness (log2 ratio) -1
0
1
2 at 110.317 kb on - strand at 110.351 kb on + strand at 110.374 kb on - strand at 110.470 kb on + strand, within GFF4329 at 110.473 kb on - strand, within GFF4329 at 110.500 kb on - strand, within GFF4329 at 110.854 kb on - strand, within GFF4329 at 110.899 kb on + strand, within GFF4329 at 110.900 kb on - strand, within GFF4329 at 110.948 kb on - strand, within GFF4329 at 111.191 kb on + strand at 111.238 kb on + strand at 111.238 kb on + strand at 111.239 kb on - strand at 111.632 kb on + strand, within GFF4330 at 111.632 kb on + strand, within GFF4330 at 111.633 kb on - strand, within GFF4330 at 111.679 kb on - strand, within GFF4330 at 111.731 kb on + strand, within GFF4330 at 111.786 kb on + strand, within GFF4330 at 111.787 kb on - strand, within GFF4330 at 111.787 kb on - strand, within GFF4330 at 111.789 kb on + strand, within GFF4330 at 111.789 kb on + strand, within GFF4330 at 111.969 kb on + strand, within GFF4330 at 111.969 kb on + strand, within GFF4330 at 112.082 kb on + strand, within GFF4330 at 112.262 kb on + strand at 112.385 kb on + strand at 112.466 kb on + strand, within GFF4331 at 112.468 kb on + strand, within GFF4331 at 112.468 kb on + strand, within GFF4331 at 112.469 kb on - strand, within GFF4331 at 112.794 kb on - strand, within GFF4331 at 112.835 kb on + strand, within GFF4331 at 112.835 kb on + strand, within GFF4331 at 112.982 kb on - strand, within GFF4331 at 112.982 kb on - strand, within GFF4331 at 113.235 kb on + strand, within GFF4331
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 110,317 - +0.4 110,351 + +0.0 110,374 - +0.8 110,470 + GFF4329 0.11 +0.3 110,473 - GFF4329 0.12 -0.3 110,500 - GFF4329 0.15 +2.0 110,854 - GFF4329 0.56 -0.1 110,899 + GFF4329 0.61 -1.3 110,900 - GFF4329 0.61 -0.5 110,948 - GFF4329 0.67 +0.0 111,191 + -1.1 111,238 + +0.9 111,238 + -0.6 111,239 - -0.1 111,632 + GFF4330 0.36 +0.3 111,632 + GFF4330 0.36 +0.4 111,633 - GFF4330 0.36 +0.4 111,679 - GFF4330 0.40 -0.4 111,731 + GFF4330 0.45 +0.3 111,786 + GFF4330 0.50 -0.4 111,787 - GFF4330 0.50 +1.0 111,787 - GFF4330 0.50 +0.9 111,789 + GFF4330 0.50 +1.4 111,789 + GFF4330 0.50 +0.6 111,969 + GFF4330 0.66 -0.2 111,969 + GFF4330 0.66 +0.4 112,082 + GFF4330 0.76 -0.0 112,262 + -0.9 112,385 + +1.5 112,466 + GFF4331 0.11 -0.4 112,468 + GFF4331 0.12 +0.3 112,468 + GFF4331 0.12 +0.4 112,469 - GFF4331 0.12 +0.0 112,794 - GFF4331 0.44 +0.6 112,835 + GFF4331 0.48 -0.0 112,835 + GFF4331 0.48 +0.4 112,982 - GFF4331 0.62 -0.9 112,982 - GFF4331 0.62 +0.8 113,235 + GFF4331 0.87 +0.2
Or see this region's nucleotide sequence