Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4273 and GFF4274 are separated by 37 nucleotides GFF4274 and GFF4275 are separated by 267 nucleotides GFF4275 and GFF4276 are separated by 25 nucleotides
GFF4273 - Putative outer membrane lipoprotein, at 61,806 to 62,900
GFF4273
GFF4274 - Putative inner membrane protein, at 62,938 to 63,246
GFF4274
GFF4275 - Putative inner membrane protein, at 63,514 to 63,729
GFF4275
GFF4276 - D-alanine--D-alanine ligase A (EC 6.3.2.4), at 63,755 to 64,849
GFF4276
Position (kb) 63
64 Strain fitness (log2 ratio) -2
-1
0
1
2 at 62.737 kb on - strand, within GFF4273 at 62.888 kb on + strand at 63.092 kb on + strand, within GFF4274 at 63.093 kb on - strand, within GFF4274 at 63.154 kb on + strand, within GFF4274 at 63.155 kb on - strand, within GFF4274 at 63.499 kb on + strand at 63.500 kb on - strand at 63.514 kb on - strand at 63.550 kb on + strand, within GFF4275 at 63.553 kb on + strand, within GFF4275 at 63.553 kb on + strand, within GFF4275 at 63.597 kb on - strand, within GFF4275 at 63.660 kb on + strand, within GFF4275 at 63.868 kb on - strand, within GFF4276 at 63.872 kb on - strand, within GFF4276 at 64.070 kb on - strand, within GFF4276 at 64.071 kb on + strand, within GFF4276 at 64.071 kb on + strand, within GFF4276 at 64.071 kb on + strand, within GFF4276 at 64.071 kb on + strand, within GFF4276 at 64.072 kb on - strand, within GFF4276 at 64.072 kb on - strand, within GFF4276 at 64.072 kb on - strand, within GFF4276 at 64.072 kb on - strand, within GFF4276 at 64.075 kb on + strand, within GFF4276 at 64.148 kb on + strand, within GFF4276 at 64.148 kb on + strand, within GFF4276 at 64.148 kb on + strand, within GFF4276 at 64.149 kb on - strand, within GFF4276 at 64.149 kb on - strand, within GFF4276 at 64.149 kb on - strand, within GFF4276 at 64.261 kb on + strand, within GFF4276 at 64.372 kb on + strand, within GFF4276 at 64.372 kb on + strand, within GFF4276 at 64.476 kb on - strand, within GFF4276 at 64.546 kb on + strand, within GFF4276 at 64.546 kb on + strand, within GFF4276 at 64.652 kb on - strand, within GFF4276 at 64.652 kb on - strand, within GFF4276 at 64.652 kb on - strand, within GFF4276 at 64.718 kb on - strand, within GFF4276 at 64.722 kb on - strand, within GFF4276
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 62,737 - GFF4273 0.85 -0.5 62,888 + +2.7 63,092 + GFF4274 0.50 +0.6 63,093 - GFF4274 0.50 -0.0 63,154 + GFF4274 0.70 +0.9 63,155 - GFF4274 0.70 +0.7 63,499 + -1.1 63,500 - +0.1 63,514 - -0.4 63,550 + GFF4275 0.17 -0.3 63,553 + GFF4275 0.18 -0.7 63,553 + GFF4275 0.18 -0.6 63,597 - GFF4275 0.38 -1.0 63,660 + GFF4275 0.68 -0.9 63,868 - GFF4276 0.10 -0.5 63,872 - GFF4276 0.11 +0.0 64,070 - GFF4276 0.29 +0.0 64,071 + GFF4276 0.29 -0.1 64,071 + GFF4276 0.29 -0.3 64,071 + GFF4276 0.29 +0.1 64,071 + GFF4276 0.29 +0.6 64,072 - GFF4276 0.29 -0.1 64,072 - GFF4276 0.29 +1.2 64,072 - GFF4276 0.29 -0.6 64,072 - GFF4276 0.29 -0.0 64,075 + GFF4276 0.29 +2.0 64,148 + GFF4276 0.36 +0.0 64,148 + GFF4276 0.36 -0.1 64,148 + GFF4276 0.36 +0.2 64,149 - GFF4276 0.36 +0.5 64,149 - GFF4276 0.36 +0.4 64,149 - GFF4276 0.36 +2.3 64,261 + GFF4276 0.46 -0.3 64,372 + GFF4276 0.56 -0.6 64,372 + GFF4276 0.56 +0.8 64,476 - GFF4276 0.66 -1.8 64,546 + GFF4276 0.72 +0.6 64,546 + GFF4276 0.72 +0.0 64,652 - GFF4276 0.82 +0.1 64,652 - GFF4276 0.82 +1.5 64,652 - GFF4276 0.82 -0.1 64,718 - GFF4276 0.88 -0.1 64,722 - GFF4276 0.88 -0.5
Or see this region's nucleotide sequence