Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4269 and GFF4270 are separated by 85 nucleotides GFF4270 and GFF4271 are separated by 44 nucleotides
GFF4269 - Putative flagellin structural protein, at 55,518 to 58,313
GFF4269
GFF4270 - FIG00637869: hypothetical protein, at 58,399 to 59,022
GFF4270
GFF4271 - Penicillin-binding protein AmpH, at 59,067 to 60,224
GFF4271
Position (kb)
58
59
60 Strain fitness (log2 ratio)
-1
0
1
2
3 at 57.417 kb on + strand, within GFF4269 at 57.422 kb on + strand, within GFF4269 at 57.488 kb on + strand, within GFF4269 at 57.547 kb on - strand, within GFF4269 at 57.585 kb on - strand, within GFF4269 at 57.585 kb on - strand, within GFF4269 at 57.602 kb on + strand, within GFF4269 at 57.650 kb on + strand, within GFF4269 at 57.651 kb on - strand, within GFF4269 at 57.744 kb on + strand, within GFF4269 at 57.744 kb on + strand, within GFF4269 at 57.744 kb on + strand, within GFF4269 at 57.839 kb on + strand, within GFF4269 at 57.843 kb on + strand, within GFF4269 at 57.843 kb on + strand, within GFF4269 at 57.844 kb on - strand, within GFF4269 at 57.844 kb on - strand, within GFF4269 at 57.844 kb on - strand, within GFF4269 at 57.928 kb on + strand, within GFF4269 at 57.928 kb on + strand, within GFF4269 at 57.928 kb on + strand, within GFF4269 at 57.929 kb on - strand, within GFF4269 at 58.055 kb on + strand at 58.055 kb on + strand at 58.065 kb on + strand at 58.066 kb on - strand at 58.066 kb on - strand at 58.141 kb on + strand at 58.168 kb on + strand at 58.249 kb on - strand at 58.299 kb on - strand at 58.304 kb on + strand at 58.398 kb on + strand at 58.399 kb on - strand at 58.519 kb on - strand, within GFF4270 at 59.048 kb on + strand at 59.139 kb on - strand at 59.192 kb on + strand, within GFF4271 at 59.215 kb on + strand, within GFF4271 at 59.283 kb on - strand, within GFF4271 at 59.284 kb on + strand, within GFF4271 at 59.284 kb on + strand, within GFF4271 at 59.396 kb on + strand, within GFF4271 at 59.423 kb on - strand, within GFF4271 at 59.462 kb on - strand, within GFF4271 at 59.462 kb on - strand, within GFF4271 at 59.492 kb on + strand, within GFF4271 at 59.499 kb on - strand, within GFF4271 at 59.615 kb on + strand, within GFF4271 at 59.615 kb on + strand, within GFF4271 at 59.616 kb on - strand, within GFF4271 at 59.852 kb on - strand, within GFF4271 at 59.882 kb on - strand, within GFF4271
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 57,417 + GFF4269 0.68 +0.2 57,422 + GFF4269 0.68 -0.1 57,488 + GFF4269 0.70 +0.8 57,547 - GFF4269 0.73 -0.0 57,585 - GFF4269 0.74 -0.5 57,585 - GFF4269 0.74 +0.5 57,602 + GFF4269 0.75 +0.5 57,650 + GFF4269 0.76 -0.3 57,651 - GFF4269 0.76 +0.1 57,744 + GFF4269 0.80 +0.2 57,744 + GFF4269 0.80 -1.4 57,744 + GFF4269 0.80 -0.2 57,839 + GFF4269 0.83 +0.8 57,843 + GFF4269 0.83 -1.3 57,843 + GFF4269 0.83 +0.4 57,844 - GFF4269 0.83 -0.4 57,844 - GFF4269 0.83 +0.7 57,844 - GFF4269 0.83 -0.2 57,928 + GFF4269 0.86 -0.0 57,928 + GFF4269 0.86 -0.6 57,928 + GFF4269 0.86 -0.6 57,929 - GFF4269 0.86 +3.0 58,055 + +1.9 58,055 + -0.8 58,065 + +0.2 58,066 - +0.8 58,066 - +0.3 58,141 + +0.1 58,168 + -0.7 58,249 - +0.0 58,299 - -0.3 58,304 + -0.9 58,398 + -0.5 58,399 - -0.3 58,519 - GFF4270 0.19 -0.8 59,048 + +0.1 59,139 - -0.7 59,192 + GFF4271 0.11 +0.5 59,215 + GFF4271 0.13 +0.1 59,283 - GFF4271 0.19 +1.3 59,284 + GFF4271 0.19 -1.2 59,284 + GFF4271 0.19 -0.3 59,396 + GFF4271 0.28 +0.1 59,423 - GFF4271 0.31 -0.2 59,462 - GFF4271 0.34 -0.2 59,462 - GFF4271 0.34 -1.4 59,492 + GFF4271 0.37 +0.4 59,499 - GFF4271 0.37 +0.6 59,615 + GFF4271 0.47 +1.0 59,615 + GFF4271 0.47 +0.2 59,616 - GFF4271 0.47 -0.3 59,852 - GFF4271 0.68 -0.9 59,882 - GFF4271 0.70 -1.6
Or see this region's nucleotide sequence