Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4241 and GFF4242 are separated by 143 nucleotides GFF4242 and GFF4243 are separated by 55 nucleotides GFF4243 and GFF4244 are separated by 147 nucleotides GFF4244 and GFF4245 are separated by 69 nucleotides
GFF4241 - Attachment invasion locus protein precursor, at 20,600 to 21,124
GFF4241
GFF4242 - Possible transcriptionl regulator, at 21,268 to 21,909
GFF4242
GFF4243 - Probable secreted protein, at 21,965 to 22,144
GFF4243
GFF4244 - Putative outer membrane lipoprotein, at 22,292 to 22,561
GFF4244
GFF4245 - RND efflux system, outer membrane lipoprotein CmeC, at 22,631 to 24,088
GFF4245
Position (kb)
21
22
23 Strain fitness (log2 ratio)
-1
0
1 at 21.200 kb on - strand at 21.806 kb on - strand, within GFF4242 at 21.859 kb on - strand at 21.984 kb on + strand, within GFF4243 at 22.269 kb on - strand at 22.323 kb on - strand, within GFF4244 at 22.576 kb on + strand at 22.579 kb on + strand at 22.579 kb on + strand at 22.579 kb on + strand at 22.580 kb on - strand at 22.580 kb on - strand at 22.582 kb on - strand at 22.624 kb on - strand at 22.691 kb on - strand at 22.746 kb on + strand at 22.754 kb on - strand at 22.774 kb on + strand at 22.780 kb on - strand, within GFF4245 at 22.879 kb on + strand, within GFF4245 at 22.928 kb on - strand, within GFF4245 at 22.991 kb on + strand, within GFF4245 at 23.072 kb on - strand, within GFF4245
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 21,200 - +0.4 21,806 - GFF4242 0.84 +0.1 21,859 - +1.0 21,984 + GFF4243 0.11 -0.9 22,269 - -0.2 22,323 - GFF4244 0.11 +0.3 22,576 + -0.3 22,579 + -0.0 22,579 + -0.4 22,579 + +0.3 22,580 - -0.4 22,580 - +1.0 22,582 - -0.6 22,624 - -0.7 22,691 - -0.4 22,746 + +0.4 22,754 - -0.2 22,774 + -0.9 22,780 - GFF4245 0.10 +0.5 22,879 + GFF4245 0.17 +0.7 22,928 - GFF4245 0.20 -0.1 22,991 + GFF4245 0.25 +0.2 23,072 - GFF4245 0.30 +0.2
Or see this region's nucleotide sequence