Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4189 and GFF4190 are separated by 253 nucleotides GFF4190 and GFF4191 are separated by 41 nucleotides GFF4191 and GFF4192 are separated by 11 nucleotides GFF4192 and GFF4193 are separated by 186 nucleotides
GFF4189 - Sensor protein of zinc sigma-54-dependent two-component system, at 4,752 to 6,149
GFF4189
GFF4190 - Zinc resistance-associated protein, at 6,403 to 6,858
GFF4190
GFF4191 - FIG00638261: hypothetical protein, at 6,900 to 7,592
GFF4191
GFF4192 - DNA-binding protein HU-alpha, at 7,604 to 7,876
GFF4192
GFF4193 - FIG01200173: hypothetical protein, at 8,063 to 8,653
GFF4193
Position (kb) 6
7
8 Strain fitness (log2 ratio) -1
0
1
2 at 5.949 kb on - strand, within GFF4189 at 5.953 kb on - strand, within GFF4189 at 5.953 kb on - strand, within GFF4189 at 5.953 kb on - strand, within GFF4189 at 5.986 kb on - strand, within GFF4189 at 5.986 kb on - strand, within GFF4189 at 5.986 kb on - strand, within GFF4189 at 6.094 kb on - strand at 6.129 kb on - strand at 6.139 kb on - strand at 6.343 kb on + strand at 6.418 kb on - strand at 6.427 kb on - strand at 6.435 kb on - strand at 6.507 kb on + strand, within GFF4190 at 6.526 kb on - strand, within GFF4190 at 6.569 kb on - strand, within GFF4190 at 6.577 kb on + strand, within GFF4190 at 6.583 kb on - strand, within GFF4190 at 6.632 kb on - strand, within GFF4190 at 6.632 kb on - strand, within GFF4190 at 7.039 kb on - strand, within GFF4191 at 7.123 kb on - strand, within GFF4191 at 7.187 kb on - strand, within GFF4191 at 7.187 kb on - strand, within GFF4191 at 7.284 kb on - strand, within GFF4191 at 7.284 kb on - strand, within GFF4191 at 7.307 kb on + strand, within GFF4191 at 7.418 kb on + strand, within GFF4191 at 7.424 kb on + strand, within GFF4191 at 7.597 kb on - strand at 7.606 kb on - strand at 7.609 kb on + strand at 7.630 kb on + strand at 7.752 kb on - strand, within GFF4192 at 7.752 kb on - strand, within GFF4192 at 7.783 kb on + strand, within GFF4192 at 7.784 kb on - strand, within GFF4192 at 7.814 kb on - strand, within GFF4192 at 7.851 kb on - strand at 7.877 kb on - strand at 7.888 kb on - strand at 7.992 kb on + strand at 8.044 kb on + strand at 8.129 kb on + strand, within GFF4193 at 8.129 kb on + strand, within GFF4193 at 8.141 kb on - strand, within GFF4193 at 8.278 kb on + strand, within GFF4193 at 8.282 kb on - strand, within GFF4193 at 8.294 kb on - strand, within GFF4193 at 8.294 kb on - strand, within GFF4193 at 8.392 kb on - strand, within GFF4193 at 8.400 kb on + strand, within GFF4193 at 8.400 kb on + strand, within GFF4193 at 8.401 kb on - strand, within GFF4193 at 8.511 kb on + strand, within GFF4193 at 8.556 kb on + strand, within GFF4193 at 8.557 kb on - strand, within GFF4193 at 8.557 kb on - strand, within GFF4193 at 8.565 kb on + strand, within GFF4193 at 8.591 kb on - strand, within GFF4193
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 5,949 - GFF4189 0.86 -1.2 5,953 - GFF4189 0.86 -0.7 5,953 - GFF4189 0.86 -0.5 5,953 - GFF4189 0.86 -0.7 5,986 - GFF4189 0.88 -0.6 5,986 - GFF4189 0.88 +0.3 5,986 - GFF4189 0.88 +1.7 6,094 - -0.0 6,129 - +0.7 6,139 - -0.2 6,343 + -1.3 6,418 - +0.3 6,427 - +0.0 6,435 - -0.7 6,507 + GFF4190 0.23 +0.1 6,526 - GFF4190 0.27 +0.2 6,569 - GFF4190 0.36 +0.8 6,577 + GFF4190 0.38 -0.9 6,583 - GFF4190 0.39 +0.1 6,632 - GFF4190 0.50 -0.2 6,632 - GFF4190 0.50 +0.2 7,039 - GFF4191 0.20 +0.0 7,123 - GFF4191 0.32 +0.2 7,187 - GFF4191 0.41 +0.2 7,187 - GFF4191 0.41 +2.7 7,284 - GFF4191 0.55 -0.1 7,284 - GFF4191 0.55 -0.9 7,307 + GFF4191 0.59 +0.3 7,418 + GFF4191 0.75 +1.7 7,424 + GFF4191 0.76 +0.3 7,597 - -0.9 7,606 - +0.1 7,609 + +0.3 7,630 + +0.6 7,752 - GFF4192 0.54 +0.3 7,752 - GFF4192 0.54 -0.0 7,783 + GFF4192 0.66 +0.3 7,784 - GFF4192 0.66 -1.1 7,814 - GFF4192 0.77 +0.3 7,851 - -0.9 7,877 - -0.4 7,888 - -0.1 7,992 + +0.1 8,044 + +1.3 8,129 + GFF4193 0.11 +0.8 8,129 + GFF4193 0.11 -0.0 8,141 - GFF4193 0.13 +0.2 8,278 + GFF4193 0.36 +1.3 8,282 - GFF4193 0.37 +0.9 8,294 - GFF4193 0.39 +0.4 8,294 - GFF4193 0.39 -0.1 8,392 - GFF4193 0.56 +0.1 8,400 + GFF4193 0.57 +0.8 8,400 + GFF4193 0.57 +1.0 8,401 - GFF4193 0.57 +1.7 8,511 + GFF4193 0.76 +1.2 8,556 + GFF4193 0.83 +0.9 8,557 - GFF4193 0.84 +0.4 8,557 - GFF4193 0.84 +0.3 8,565 + GFF4193 0.85 +0.6 8,591 - GFF4193 0.89 +0.1
Or see this region's nucleotide sequence