Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF414 and GFF415 are separated by 290 nucleotides GFF415 and GFF416 are separated by 9 nucleotides GFF416 and GFF417 overlap by 4 nucleotides
GFF414 - FIG00638433: Hypothetical protein YibL, at 133,067 to 133,429
GFF414
GFF415 - FIG074102: hypothetical protein, at 133,720 to 133,929
GFF415
GFF416 - Mannitol operon repressor, at 133,939 to 134,529
GFF416
GFF417 - Mannitol-1-phosphate 5-dehydrogenase (EC 1.1.1.17), at 134,526 to 135,674
GFF417
Position (kb) 133
134 Strain fitness (log2 ratio) -2
-1
0
1
2 at 132.963 kb on + strand at 132.965 kb on + strand at 132.978 kb on + strand at 133.265 kb on + strand, within GFF414 at 133.265 kb on + strand, within GFF414 at 133.265 kb on + strand, within GFF414 at 133.283 kb on - strand, within GFF414 at 133.295 kb on + strand, within GFF414 at 133.296 kb on - strand, within GFF414 at 133.326 kb on + strand, within GFF414 at 133.375 kb on + strand, within GFF414 at 133.455 kb on + strand at 133.460 kb on - strand at 133.460 kb on - strand at 133.466 kb on + strand at 133.467 kb on - strand at 133.470 kb on - strand at 133.507 kb on - strand at 133.674 kb on - strand at 133.716 kb on + strand at 133.716 kb on + strand at 133.828 kb on + strand, within GFF415 at 133.828 kb on + strand, within GFF415 at 134.045 kb on + strand, within GFF416 at 134.148 kb on - strand, within GFF416 at 134.148 kb on - strand, within GFF416 at 134.208 kb on - strand, within GFF416 at 134.237 kb on + strand, within GFF416 at 134.242 kb on - strand, within GFF416 at 134.242 kb on - strand, within GFF416 at 134.242 kb on - strand, within GFF416 at 134.255 kb on - strand, within GFF416 at 134.306 kb on + strand, within GFF416 at 134.319 kb on - strand, within GFF416 at 134.464 kb on + strand, within GFF416 at 134.475 kb on + strand at 134.476 kb on - strand at 134.670 kb on - strand, within GFF417 at 134.710 kb on - strand, within GFF417 at 134.710 kb on - strand, within GFF417 at 134.725 kb on + strand, within GFF417 at 134.796 kb on + strand, within GFF417 at 134.857 kb on + strand, within GFF417 at 134.858 kb on - strand, within GFF417
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 132,963 + +1.0 132,965 + -0.9 132,978 + -0.6 133,265 + GFF414 0.55 -0.7 133,265 + GFF414 0.55 -0.5 133,265 + GFF414 0.55 -0.1 133,283 - GFF414 0.60 +0.5 133,295 + GFF414 0.63 -0.5 133,296 - GFF414 0.63 +0.7 133,326 + GFF414 0.71 -1.1 133,375 + GFF414 0.85 +1.2 133,455 + -0.7 133,460 - -2.0 133,460 - -2.2 133,466 + -0.2 133,467 - -0.0 133,470 - +0.2 133,507 - +0.3 133,674 - -1.2 133,716 + -0.0 133,716 + -2.1 133,828 + GFF415 0.51 -1.1 133,828 + GFF415 0.51 -0.6 134,045 + GFF416 0.18 +0.8 134,148 - GFF416 0.35 +0.5 134,148 - GFF416 0.35 -0.3 134,208 - GFF416 0.46 +0.4 134,237 + GFF416 0.50 -1.7 134,242 - GFF416 0.51 -0.5 134,242 - GFF416 0.51 -0.9 134,242 - GFF416 0.51 +0.4 134,255 - GFF416 0.53 +0.6 134,306 + GFF416 0.62 -0.4 134,319 - GFF416 0.64 -0.0 134,464 + GFF416 0.89 -0.2 134,475 + +2.2 134,476 - -0.8 134,670 - GFF417 0.13 -0.5 134,710 - GFF417 0.16 -0.6 134,710 - GFF417 0.16 -0.1 134,725 + GFF417 0.17 +2.1 134,796 + GFF417 0.23 -1.2 134,857 + GFF417 0.29 +0.4 134,858 - GFF417 0.29 +1.1
Or see this region's nucleotide sequence