Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF412 and GFF413 are separated by 43 nucleotides GFF413 and GFF414 are separated by 489 nucleotides
GFF412 - Peptide transport system permease protein sapB (TC 3.A.1.5.5), at 127,465 to 131,850
GFF412
GFF413 - Putative lipoprotein, at 131,894 to 132,577
GFF413
GFF414 - FIG00638433: Hypothetical protein YibL, at 133,067 to 133,429
GFF414
Position (kb) 131
132
133 Strain fitness (log2 ratio) -2
-1
0
1
2 at 130.953 kb on + strand, within GFF412 at 130.954 kb on - strand, within GFF412 at 130.963 kb on + strand, within GFF412 at 130.964 kb on - strand, within GFF412 at 130.964 kb on - strand, within GFF412 at 130.971 kb on + strand, within GFF412 at 130.972 kb on - strand, within GFF412 at 130.975 kb on + strand, within GFF412 at 130.975 kb on + strand, within GFF412 at 130.976 kb on - strand, within GFF412 at 130.985 kb on + strand, within GFF412 at 130.985 kb on + strand, within GFF412 at 131.000 kb on - strand, within GFF412 at 131.007 kb on - strand, within GFF412 at 131.026 kb on - strand, within GFF412 at 131.026 kb on - strand, within GFF412 at 131.080 kb on + strand, within GFF412 at 131.116 kb on - strand, within GFF412 at 131.147 kb on + strand, within GFF412 at 131.147 kb on + strand, within GFF412 at 131.147 kb on + strand, within GFF412 at 131.147 kb on + strand, within GFF412 at 131.147 kb on + strand, within GFF412 at 131.148 kb on - strand, within GFF412 at 131.148 kb on - strand, within GFF412 at 131.148 kb on - strand, within GFF412 at 131.148 kb on - strand, within GFF412 at 131.148 kb on - strand, within GFF412 at 131.148 kb on - strand, within GFF412 at 131.148 kb on - strand, within GFF412 at 131.148 kb on - strand, within GFF412 at 131.300 kb on + strand, within GFF412 at 131.300 kb on + strand, within GFF412 at 131.305 kb on + strand, within GFF412 at 131.305 kb on + strand, within GFF412 at 131.306 kb on - strand, within GFF412 at 131.306 kb on - strand, within GFF412 at 131.340 kb on + strand, within GFF412 at 131.340 kb on + strand, within GFF412 at 131.341 kb on - strand, within GFF412 at 131.347 kb on + strand, within GFF412 at 131.382 kb on - strand, within GFF412 at 131.403 kb on - strand, within GFF412 at 131.550 kb on + strand at 131.551 kb on - strand at 131.593 kb on - strand at 131.836 kb on - strand at 132.224 kb on - strand, within GFF413 at 132.301 kb on + strand, within GFF413 at 132.301 kb on + strand, within GFF413 at 132.963 kb on + strand at 132.965 kb on + strand at 132.978 kb on + strand at 133.265 kb on + strand, within GFF414 at 133.265 kb on + strand, within GFF414 at 133.265 kb on + strand, within GFF414 at 133.283 kb on - strand, within GFF414 at 133.295 kb on + strand, within GFF414 at 133.296 kb on - strand, within GFF414 at 133.326 kb on + strand, within GFF414 at 133.375 kb on + strand, within GFF414 at 133.455 kb on + strand at 133.460 kb on - strand at 133.460 kb on - strand at 133.466 kb on + strand at 133.467 kb on - strand at 133.470 kb on - strand at 133.507 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 130,953 + GFF412 0.80 +0.1 130,954 - GFF412 0.80 -0.5 130,963 + GFF412 0.80 -0.2 130,964 - GFF412 0.80 -1.5 130,964 - GFF412 0.80 -1.9 130,971 + GFF412 0.80 +1.6 130,972 - GFF412 0.80 -0.9 130,975 + GFF412 0.80 +0.1 130,975 + GFF412 0.80 -0.6 130,976 - GFF412 0.80 +0.0 130,985 + GFF412 0.80 -0.7 130,985 + GFF412 0.80 +1.1 131,000 - GFF412 0.81 -0.8 131,007 - GFF412 0.81 -0.4 131,026 - GFF412 0.81 -0.9 131,026 - GFF412 0.81 -0.6 131,080 + GFF412 0.82 +2.0 131,116 - GFF412 0.83 -0.3 131,147 + GFF412 0.84 +0.1 131,147 + GFF412 0.84 -0.2 131,147 + GFF412 0.84 +0.2 131,147 + GFF412 0.84 -0.0 131,147 + GFF412 0.84 -0.9 131,148 - GFF412 0.84 -0.8 131,148 - GFF412 0.84 -0.5 131,148 - GFF412 0.84 -0.6 131,148 - GFF412 0.84 +1.3 131,148 - GFF412 0.84 +1.7 131,148 - GFF412 0.84 -0.2 131,148 - GFF412 0.84 +0.3 131,148 - GFF412 0.84 -0.7 131,300 + GFF412 0.87 -0.2 131,300 + GFF412 0.87 +0.4 131,305 + GFF412 0.88 +0.3 131,305 + GFF412 0.88 +0.0 131,306 - GFF412 0.88 -1.0 131,306 - GFF412 0.88 +0.3 131,340 + GFF412 0.88 +0.3 131,340 + GFF412 0.88 -0.1 131,341 - GFF412 0.88 -0.4 131,347 + GFF412 0.89 -1.2 131,382 - GFF412 0.89 -0.3 131,403 - GFF412 0.90 -0.3 131,550 + +1.0 131,551 - -0.4 131,593 - +0.1 131,836 - +0.5 132,224 - GFF413 0.48 -0.3 132,301 + GFF413 0.60 -0.1 132,301 + GFF413 0.60 +1.5 132,963 + +1.0 132,965 + -0.9 132,978 + -0.6 133,265 + GFF414 0.55 -0.7 133,265 + GFF414 0.55 -0.5 133,265 + GFF414 0.55 -0.1 133,283 - GFF414 0.60 +0.5 133,295 + GFF414 0.63 -0.5 133,296 - GFF414 0.63 +0.7 133,326 + GFF414 0.71 -1.1 133,375 + GFF414 0.85 +1.2 133,455 + -0.7 133,460 - -2.0 133,460 - -2.2 133,466 + -0.2 133,467 - -0.0 133,470 - +0.2 133,507 - +0.3
Or see this region's nucleotide sequence