Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4108 and GFF4109 are separated by 248 nucleotides GFF4109 and GFF4110 are separated by 88 nucleotides
GFF4108 - Aminoacyl-histidine dipeptidase (Peptidase D) (EC 3.4.13.3), at 66,383 to 67,840
GFF4108
GFF4109 - Xanthine-guanine phosphoribosyltransferase (EC 2.4.2.22), at 68,089 to 68,547
GFF4109
GFF4110 - Fermentation/respiration switch protein, at 68,636 to 69,880
GFF4110
Position (kb) 68
69 Strain fitness (log2 ratio) -2
-1
0
1
2 at 67.229 kb on - strand, within GFF4108 at 67.312 kb on - strand, within GFF4108 at 67.312 kb on - strand, within GFF4108 at 67.450 kb on + strand, within GFF4108 at 67.451 kb on - strand, within GFF4108 at 67.451 kb on - strand, within GFF4108 at 67.543 kb on + strand, within GFF4108 at 67.543 kb on + strand, within GFF4108 at 67.544 kb on - strand, within GFF4108 at 67.544 kb on - strand, within GFF4108 at 67.544 kb on - strand, within GFF4108 at 67.544 kb on - strand, within GFF4108 at 67.545 kb on + strand, within GFF4108 at 67.546 kb on - strand, within GFF4108 at 67.546 kb on - strand, within GFF4108 at 67.546 kb on - strand, within GFF4108 at 67.546 kb on - strand, within GFF4108 at 67.556 kb on - strand, within GFF4108 at 67.649 kb on - strand, within GFF4108 at 67.704 kb on + strand at 67.705 kb on - strand at 67.778 kb on - strand at 67.778 kb on - strand at 67.843 kb on + strand at 67.843 kb on + strand at 67.843 kb on + strand at 67.844 kb on - strand at 67.911 kb on + strand at 67.911 kb on + strand at 67.911 kb on + strand at 67.955 kb on + strand at 67.958 kb on - strand at 67.958 kb on - strand at 68.034 kb on + strand at 68.034 kb on + strand at 68.035 kb on - strand at 68.035 kb on - strand at 68.276 kb on - strand, within GFF4109 at 68.399 kb on - strand, within GFF4109 at 68.462 kb on - strand, within GFF4109 at 68.467 kb on - strand, within GFF4109 at 68.602 kb on + strand at 68.602 kb on + strand at 68.617 kb on - strand at 68.698 kb on + strand at 68.788 kb on - strand, within GFF4110 at 68.890 kb on - strand, within GFF4110 at 69.030 kb on - strand, within GFF4110 at 69.030 kb on - strand, within GFF4110 at 69.030 kb on - strand, within GFF4110 at 69.032 kb on - strand, within GFF4110 at 69.032 kb on - strand, within GFF4110 at 69.032 kb on - strand, within GFF4110 at 69.032 kb on - strand, within GFF4110 at 69.194 kb on - strand, within GFF4110 at 69.263 kb on + strand, within GFF4110 at 69.266 kb on + strand, within GFF4110 at 69.266 kb on + strand, within GFF4110 at 69.323 kb on - strand, within GFF4110 at 69.428 kb on - strand, within GFF4110 at 69.543 kb on + strand, within GFF4110 at 69.547 kb on + strand, within GFF4110
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 67,229 - GFF4108 0.58 -0.2 67,312 - GFF4108 0.64 -0.6 67,312 - GFF4108 0.64 -0.7 67,450 + GFF4108 0.73 -0.4 67,451 - GFF4108 0.73 +0.4 67,451 - GFF4108 0.73 -0.7 67,543 + GFF4108 0.80 +0.1 67,543 + GFF4108 0.80 -0.9 67,544 - GFF4108 0.80 -0.7 67,544 - GFF4108 0.80 +0.8 67,544 - GFF4108 0.80 +0.3 67,544 - GFF4108 0.80 -0.4 67,545 + GFF4108 0.80 -0.3 67,546 - GFF4108 0.80 -1.3 67,546 - GFF4108 0.80 -0.1 67,546 - GFF4108 0.80 +0.2 67,546 - GFF4108 0.80 +0.0 67,556 - GFF4108 0.80 +0.3 67,649 - GFF4108 0.87 -0.9 67,704 + -0.3 67,705 - -0.0 67,778 - -0.9 67,778 - +0.4 67,843 + +0.0 67,843 + -0.7 67,843 + +0.0 67,844 - +0.4 67,911 + +0.2 67,911 + -0.8 67,911 + -0.2 67,955 + +0.2 67,958 - +0.2 67,958 - +0.6 68,034 + +0.0 68,034 + -0.0 68,035 - -0.1 68,035 - -0.1 68,276 - GFF4109 0.41 -0.7 68,399 - GFF4109 0.68 -0.3 68,462 - GFF4109 0.81 -2.5 68,467 - GFF4109 0.82 -0.3 68,602 + -0.0 68,602 + -0.2 68,617 - -0.4 68,698 + -0.9 68,788 - GFF4110 0.12 -0.5 68,890 - GFF4110 0.20 +0.0 69,030 - GFF4110 0.32 -2.7 69,030 - GFF4110 0.32 -1.1 69,030 - GFF4110 0.32 -0.3 69,032 - GFF4110 0.32 -0.5 69,032 - GFF4110 0.32 +0.5 69,032 - GFF4110 0.32 -1.0 69,032 - GFF4110 0.32 -0.8 69,194 - GFF4110 0.45 +1.5 69,263 + GFF4110 0.50 -0.0 69,266 + GFF4110 0.51 -1.7 69,266 + GFF4110 0.51 -0.4 69,323 - GFF4110 0.55 -0.4 69,428 - GFF4110 0.64 -2.0 69,543 + GFF4110 0.73 +1.9 69,547 + GFF4110 0.73 -0.0
Or see this region's nucleotide sequence