Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4068 and GFF4069 overlap by 1 nucleotides GFF4069 and GFF4070 are separated by 26 nucleotides GFF4070 and GFF4071 are separated by 26 nucleotides GFF4071 and GFF4072 are separated by 396 nucleotides
GFF4068 - IcmF-related protein, at 29,271 to 33,140
GFF4068
GFF4069 - FIG074102: hypothetical protein, at 33,140 to 33,928
GFF4069
GFF4070 - Probable secreted protein, at 33,955 to 34,341
GFF4070
GFF4071 - FIG074102: hypothetical protein, at 34,368 to 34,886
GFF4071
GFF4072 - VgrG protein, at 35,283 to 37,472
GFF4072
Position (kb) 33
34
35 Strain fitness (log2 ratio) -1
0
1 at 33.084 kb on - strand at 33.203 kb on + strand at 33.367 kb on + strand, within GFF4069 at 33.368 kb on - strand, within GFF4069 at 33.512 kb on + strand, within GFF4069 at 33.602 kb on - strand, within GFF4069 at 33.765 kb on + strand, within GFF4069 at 33.876 kb on - strand at 33.910 kb on + strand at 33.954 kb on + strand at 33.986 kb on + strand at 34.011 kb on - strand, within GFF4070 at 34.080 kb on + strand, within GFF4070 at 34.081 kb on - strand, within GFF4070 at 34.146 kb on - strand, within GFF4070 at 34.225 kb on - strand, within GFF4070 at 34.268 kb on + strand, within GFF4070 at 34.268 kb on + strand, within GFF4070 at 34.268 kb on + strand, within GFF4070 at 34.319 kb on + strand at 34.319 kb on + strand at 34.319 kb on + strand at 34.351 kb on + strand at 34.428 kb on - strand, within GFF4071 at 34.508 kb on + strand, within GFF4071 at 34.650 kb on - strand, within GFF4071 at 34.719 kb on + strand, within GFF4071
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 33,084 - -0.9 33,203 + +0.1 33,367 + GFF4069 0.29 -1.0 33,368 - GFF4069 0.29 +0.5 33,512 + GFF4069 0.47 +0.3 33,602 - GFF4069 0.59 +0.7 33,765 + GFF4069 0.79 +0.3 33,876 - +0.0 33,910 + +0.2 33,954 + +0.4 33,986 + +0.1 34,011 - GFF4070 0.14 -0.7 34,080 + GFF4070 0.32 +0.2 34,081 - GFF4070 0.33 +1.4 34,146 - GFF4070 0.49 +1.4 34,225 - GFF4070 0.70 -0.6 34,268 + GFF4070 0.81 -0.1 34,268 + GFF4070 0.81 +1.4 34,268 + GFF4070 0.81 -0.1 34,319 + +0.5 34,319 + +0.4 34,319 + -0.4 34,351 + +0.9 34,428 - GFF4071 0.12 -0.1 34,508 + GFF4071 0.27 +0.8 34,650 - GFF4071 0.54 -0.9 34,719 + GFF4071 0.68 +0.3
Or see this region's nucleotide sequence