Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3959 and GFF3960 are separated by 2 nucleotides GFF3960 and GFF3961 overlap by 4 nucleotides GFF3961 and GFF3962 overlap by 4 nucleotides GFF3962 and GFF3963 are separated by 1 nucleotides
GFF3959 - Cobalt-precorrin-6x reductase (EC 1.3.1.54), at 238,851 to 239,642
GFF3959
GFF3960 - Sirohydrochlorin cobaltochelatase CbiK (EC 4.99.1.3), at 239,645 to 240,439
GFF3960
GFF3961 - Cobalt-precorrin-2 C20-methyltransferase (EC 2.1.1.130), at 240,436 to 241,149
GFF3961
GFF3962 - Substrate-specific component CbiM of cobalt ECF transporter, at 241,146 to 241,883
GFF3962
GFF3963 - Additional substrate-specific component CbiN of cobalt ECF transporter, at 241,885 to 242,166
GFF3963
Position (kb) 240
241
242 Strain fitness (log2 ratio) -1
0
1 at 239.685 kb on - strand at 240.003 kb on - strand, within GFF3960 at 240.003 kb on - strand, within GFF3960 at 240.107 kb on + strand, within GFF3960 at 240.108 kb on - strand, within GFF3960 at 240.125 kb on - strand, within GFF3960 at 240.459 kb on - strand at 240.611 kb on + strand, within GFF3961 at 240.612 kb on - strand, within GFF3961 at 240.724 kb on - strand, within GFF3961 at 240.864 kb on - strand, within GFF3961 at 240.927 kb on - strand, within GFF3961 at 241.125 kb on + strand at 241.126 kb on - strand at 241.139 kb on - strand at 241.288 kb on + strand, within GFF3962 at 241.297 kb on + strand, within GFF3962 at 241.466 kb on + strand, within GFF3962 at 242.019 kb on + strand, within GFF3963 at 242.019 kb on + strand, within GFF3963 at 242.020 kb on - strand, within GFF3963 at 242.020 kb on - strand, within GFF3963 at 242.039 kb on + strand, within GFF3963
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 239,685 - -0.5 240,003 - GFF3960 0.45 -1.1 240,003 - GFF3960 0.45 -1.3 240,107 + GFF3960 0.58 -0.8 240,108 - GFF3960 0.58 -0.5 240,125 - GFF3960 0.60 -0.8 240,459 - -0.1 240,611 + GFF3961 0.25 -0.6 240,612 - GFF3961 0.25 -0.4 240,724 - GFF3961 0.40 -1.5 240,864 - GFF3961 0.60 -0.2 240,927 - GFF3961 0.69 -0.7 241,125 + +0.1 241,126 - -0.6 241,139 - -0.6 241,288 + GFF3962 0.19 +1.0 241,297 + GFF3962 0.20 -0.2 241,466 + GFF3962 0.43 -0.1 242,019 + GFF3963 0.48 +0.2 242,019 + GFF3963 0.48 -0.6 242,020 - GFF3963 0.48 +0.5 242,020 - GFF3963 0.48 +0.3 242,039 + GFF3963 0.55 +0.1
Or see this region's nucleotide sequence