Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3820 and GFF3821 are separated by 46 nucleotides GFF3821 and GFF3822 are separated by 56 nucleotides GFF3822 and GFF3823 are separated by 279 nucleotides GFF3823 and GFF3824 are separated by 240 nucleotides
GFF3820 - Two-component response-regulatory protein YehT, at 90,389 to 91,108
GFF3820
GFF3821 - FIG00638377: hypothetical protein, at 91,155 to 91,622
GFF3821
GFF3822 - FIG00545237: hypothetical protein, at 91,679 to 92,101
GFF3822
GFF3823 - Hypothetical lipoprotein yehR, at 92,381 to 92,839
GFF3823
GFF3824 - Methionyl-tRNA synthetase (EC 6.1.1.10), at 93,080 to 95,194
GFF3824
Position (kb)
91
92
93 Strain fitness (log2 ratio)
-1
0
1
2
3 at 90.724 kb on - strand, within GFF3820 at 90.866 kb on - strand, within GFF3820 at 90.982 kb on + strand, within GFF3820 at 91.205 kb on + strand, within GFF3821 at 91.205 kb on + strand, within GFF3821 at 91.415 kb on + strand, within GFF3821 at 91.415 kb on + strand, within GFF3821 at 91.416 kb on - strand, within GFF3821 at 91.436 kb on + strand, within GFF3821 at 91.463 kb on + strand, within GFF3821 at 91.479 kb on - strand, within GFF3821 at 91.542 kb on - strand, within GFF3821 at 91.675 kb on + strand at 91.676 kb on - strand at 91.676 kb on - strand at 91.677 kb on + strand at 91.740 kb on + strand, within GFF3822 at 91.781 kb on - strand, within GFF3822 at 91.837 kb on - strand, within GFF3822 at 91.840 kb on + strand, within GFF3822 at 91.951 kb on - strand, within GFF3822 at 91.951 kb on - strand, within GFF3822 at 92.044 kb on - strand, within GFF3822 at 92.365 kb on - strand at 92.370 kb on - strand at 92.539 kb on - strand, within GFF3823 at 92.539 kb on - strand, within GFF3823 at 92.539 kb on - strand, within GFF3823 at 92.652 kb on + strand, within GFF3823 at 92.917 kb on - strand at 92.950 kb on + strand at 92.983 kb on - strand at 93.025 kb on + strand at 93.081 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 90,724 - GFF3820 0.47 +0.5 90,866 - GFF3820 0.66 +1.1 90,982 + GFF3820 0.82 +0.6 91,205 + GFF3821 0.11 -0.7 91,205 + GFF3821 0.11 -0.1 91,415 + GFF3821 0.56 +0.3 91,415 + GFF3821 0.56 +0.2 91,416 - GFF3821 0.56 -0.8 91,436 + GFF3821 0.60 +0.0 91,463 + GFF3821 0.66 -1.0 91,479 - GFF3821 0.69 +3.0 91,542 - GFF3821 0.83 +2.1 91,675 + -0.5 91,676 - +0.0 91,676 - -0.3 91,677 + +0.2 91,740 + GFF3822 0.14 -1.0 91,781 - GFF3822 0.24 -0.3 91,837 - GFF3822 0.37 -0.8 91,840 + GFF3822 0.38 +0.3 91,951 - GFF3822 0.64 +0.5 91,951 - GFF3822 0.64 -1.4 92,044 - GFF3822 0.86 -0.5 92,365 - -0.7 92,370 - +0.7 92,539 - GFF3823 0.34 -0.3 92,539 - GFF3823 0.34 -1.3 92,539 - GFF3823 0.34 +0.1 92,652 + GFF3823 0.59 -0.2 92,917 - +0.4 92,950 + +2.1 92,983 - -0.6 93,025 + +1.0 93,081 + -0.4
Or see this region's nucleotide sequence