Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3813 and GFF3814 are separated by 180 nucleotides GFF3814 and GFF3815 are separated by 3 nucleotides
GFF3813 - Periplasmic beta-glucosidase (EC 3.2.1.21), at 81,412 to 83,679
GFF3813
GFF3814 - Osmoprotectant ABC transporter binding protein YehZ, at 83,860 to 84,777
GFF3814
GFF3815 - Osmoprotectant ABC transporter permease protein YehY, at 84,781 to 85,950
GFF3815
Position (kb)
83
84
85 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 82.869 kb on - strand, within GFF3813 at 83.008 kb on - strand, within GFF3813 at 83.019 kb on - strand, within GFF3813 at 83.027 kb on + strand, within GFF3813 at 83.027 kb on + strand, within GFF3813 at 83.239 kb on - strand, within GFF3813 at 83.260 kb on + strand, within GFF3813 at 83.324 kb on - strand, within GFF3813 at 83.324 kb on - strand, within GFF3813 at 83.324 kb on - strand, within GFF3813 at 83.334 kb on + strand, within GFF3813 at 83.449 kb on + strand, within GFF3813 at 83.536 kb on - strand at 83.672 kb on + strand at 83.746 kb on - strand at 83.877 kb on - strand at 83.915 kb on + strand at 84.207 kb on + strand, within GFF3814 at 84.207 kb on + strand, within GFF3814 at 84.285 kb on + strand, within GFF3814 at 84.583 kb on - strand, within GFF3814 at 84.620 kb on + strand, within GFF3814 at 84.621 kb on - strand, within GFF3814 at 84.749 kb on - strand at 85.183 kb on - strand, within GFF3815 at 85.183 kb on - strand, within GFF3815 at 85.289 kb on - strand, within GFF3815 at 85.289 kb on - strand, within GFF3815 at 85.289 kb on - strand, within GFF3815 at 85.380 kb on - strand, within GFF3815 at 85.380 kb on - strand, within GFF3815 at 85.456 kb on - strand, within GFF3815 at 85.585 kb on + strand, within GFF3815 at 85.586 kb on - strand, within GFF3815 at 85.731 kb on - strand, within GFF3815 at 85.748 kb on + strand, within GFF3815 at 85.749 kb on - strand, within GFF3815 at 85.749 kb on - strand, within GFF3815
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 82,869 - GFF3813 0.64 -0.9 83,008 - GFF3813 0.70 +0.9 83,019 - GFF3813 0.71 +1.3 83,027 + GFF3813 0.71 -0.5 83,027 + GFF3813 0.71 +0.5 83,239 - GFF3813 0.81 +0.4 83,260 + GFF3813 0.81 -2.8 83,324 - GFF3813 0.84 +0.2 83,324 - GFF3813 0.84 +0.5 83,324 - GFF3813 0.84 -1.1 83,334 + GFF3813 0.85 +0.2 83,449 + GFF3813 0.90 +0.5 83,536 - +0.5 83,672 + +0.3 83,746 - -0.4 83,877 - -0.2 83,915 + +0.6 84,207 + GFF3814 0.38 -0.5 84,207 + GFF3814 0.38 -1.0 84,285 + GFF3814 0.46 +0.3 84,583 - GFF3814 0.79 -1.5 84,620 + GFF3814 0.83 -1.1 84,621 - GFF3814 0.83 -0.3 84,749 - +0.1 85,183 - GFF3815 0.34 +2.5 85,183 - GFF3815 0.34 -1.8 85,289 - GFF3815 0.43 -2.8 85,289 - GFF3815 0.43 +0.4 85,289 - GFF3815 0.43 -0.1 85,380 - GFF3815 0.51 +0.5 85,380 - GFF3815 0.51 -1.2 85,456 - GFF3815 0.58 +1.8 85,585 + GFF3815 0.69 +0.8 85,586 - GFF3815 0.69 -0.1 85,731 - GFF3815 0.81 -0.1 85,748 + GFF3815 0.83 -0.0 85,749 - GFF3815 0.83 +0.2 85,749 - GFF3815 0.83 -0.7
Or see this region's nucleotide sequence