Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3762 and GFF3763 are separated by 2 nucleotides GFF3763 and GFF3764 are separated by 185 nucleotides
GFF3762 - Putative membrane protein, at 31,607 to 32,353
GFF3762
GFF3763 - Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family, at 32,356 to 33,369
GFF3763
GFF3764 - Translation elongation factor P-related protein, at 33,555 to 34,127
GFF3764
Position (kb) 32
33
34 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 31.553 kb on - strand at 31.571 kb on + strand at 31.794 kb on - strand, within GFF3762 at 31.942 kb on + strand, within GFF3762 at 32.149 kb on + strand, within GFF3762 at 32.298 kb on - strand at 32.358 kb on - strand at 32.521 kb on + strand, within GFF3763 at 32.521 kb on + strand, within GFF3763 at 32.522 kb on - strand, within GFF3763 at 32.522 kb on - strand, within GFF3763 at 32.530 kb on - strand, within GFF3763 at 32.764 kb on + strand, within GFF3763 at 32.785 kb on + strand, within GFF3763 at 32.887 kb on - strand, within GFF3763 at 32.898 kb on + strand, within GFF3763 at 32.898 kb on + strand, within GFF3763 at 33.031 kb on + strand, within GFF3763 at 33.214 kb on - strand, within GFF3763 at 33.268 kb on - strand at 33.275 kb on - strand at 33.275 kb on - strand at 33.277 kb on + strand at 33.369 kb on + strand at 33.509 kb on - strand at 33.556 kb on + strand at 33.575 kb on - strand at 33.695 kb on + strand, within GFF3764 at 33.810 kb on - strand, within GFF3764 at 33.848 kb on + strand, within GFF3764 at 33.848 kb on + strand, within GFF3764 at 33.848 kb on + strand, within GFF3764 at 33.850 kb on + strand, within GFF3764 at 33.859 kb on + strand, within GFF3764 at 33.883 kb on + strand, within GFF3764 at 33.883 kb on + strand, within GFF3764 at 34.235 kb on - strand at 34.236 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 31,553 - +0.1 31,571 + +0.5 31,794 - GFF3762 0.25 -0.0 31,942 + GFF3762 0.45 -3.3 32,149 + GFF3762 0.73 +0.2 32,298 - -0.5 32,358 - +0.5 32,521 + GFF3763 0.16 +1.4 32,521 + GFF3763 0.16 -0.7 32,522 - GFF3763 0.16 +0.6 32,522 - GFF3763 0.16 +0.9 32,530 - GFF3763 0.17 +0.1 32,764 + GFF3763 0.40 -0.7 32,785 + GFF3763 0.42 -0.3 32,887 - GFF3763 0.52 -0.4 32,898 + GFF3763 0.53 -0.3 32,898 + GFF3763 0.53 +0.9 33,031 + GFF3763 0.67 +0.4 33,214 - GFF3763 0.85 +2.8 33,268 - +1.1 33,275 - +0.3 33,275 - +0.4 33,277 + -0.2 33,369 + +0.3 33,509 - +2.3 33,556 + -0.0 33,575 - +0.2 33,695 + GFF3764 0.24 -0.6 33,810 - GFF3764 0.45 +0.2 33,848 + GFF3764 0.51 -0.8 33,848 + GFF3764 0.51 -0.5 33,848 + GFF3764 0.51 +0.2 33,850 + GFF3764 0.51 +0.8 33,859 + GFF3764 0.53 -0.7 33,883 + GFF3764 0.57 -0.5 33,883 + GFF3764 0.57 +0.4 34,235 - -0.8 34,236 - +0.5
Or see this region's nucleotide sequence