Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3712 and GFF3713 are separated by 307 nucleotides GFF3713 and GFF3714 are separated by 95 nucleotides GFF3714 and GFF3715 are separated by 51 nucleotides GFF3715 and GFF3716 overlap by 4 nucleotides
GFF3712 - Cyclic AMP receptor protein, at 18,664 to 19,296
GFF3712
GFF3713 - OsmC/Ohr family protein, at 19,604 to 20,008
GFF3713
GFF3714 - Phosphoribulokinase (EC 2.7.1.19) homolog, function unknown, at 20,104 to 20,973
GFF3714
GFF3715 - FIG01199774: hypothetical protein, at 21,025 to 21,243
GFF3715
GFF3716 - Hydrolase, alpha/beta fold family functionally coupled to Phosphoribulokinase, at 21,240 to 22,199
GFF3716
Position (kb) 20
21 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 19.172 kb on + strand, within GFF3712 at 19.172 kb on + strand, within GFF3712 at 19.173 kb on - strand, within GFF3712 at 19.173 kb on - strand, within GFF3712 at 19.173 kb on - strand, within GFF3712 at 19.315 kb on - strand at 19.317 kb on - strand at 19.317 kb on - strand at 19.402 kb on + strand at 19.417 kb on - strand at 19.455 kb on - strand at 19.455 kb on - strand at 19.457 kb on + strand at 19.457 kb on + strand at 19.463 kb on - strand at 19.479 kb on + strand at 19.557 kb on + strand at 19.557 kb on + strand at 19.558 kb on - strand at 19.558 kb on - strand at 19.565 kb on + strand at 19.722 kb on + strand, within GFF3713 at 19.722 kb on + strand, within GFF3713 at 19.722 kb on + strand, within GFF3713 at 19.725 kb on + strand, within GFF3713 at 19.725 kb on + strand, within GFF3713 at 19.745 kb on - strand, within GFF3713 at 19.859 kb on - strand, within GFF3713 at 19.859 kb on - strand, within GFF3713 at 19.871 kb on - strand, within GFF3713 at 20.151 kb on + strand at 20.151 kb on + strand at 20.152 kb on - strand at 20.194 kb on + strand, within GFF3714 at 20.195 kb on - strand, within GFF3714 at 20.195 kb on - strand, within GFF3714 at 20.199 kb on + strand, within GFF3714 at 20.397 kb on - strand, within GFF3714 at 20.508 kb on + strand, within GFF3714 at 20.508 kb on + strand, within GFF3714 at 20.632 kb on - strand, within GFF3714 at 20.672 kb on + strand, within GFF3714 at 20.673 kb on - strand, within GFF3714 at 20.673 kb on - strand, within GFF3714 at 20.677 kb on - strand, within GFF3714 at 20.677 kb on - strand, within GFF3714 at 20.677 kb on - strand, within GFF3714 at 20.677 kb on - strand, within GFF3714 at 20.677 kb on - strand, within GFF3714 at 20.677 kb on - strand, within GFF3714 at 20.688 kb on + strand, within GFF3714 at 20.703 kb on + strand, within GFF3714 at 20.724 kb on + strand, within GFF3714 at 20.730 kb on + strand, within GFF3714 at 20.731 kb on - strand, within GFF3714 at 20.731 kb on - strand, within GFF3714 at 20.779 kb on - strand, within GFF3714 at 20.835 kb on - strand, within GFF3714 at 20.945 kb on - strand at 21.002 kb on - strand at 21.002 kb on - strand at 21.002 kb on - strand at 21.007 kb on - strand at 21.070 kb on + strand, within GFF3715 at 21.162 kb on - strand, within GFF3715 at 21.259 kb on + strand at 21.413 kb on + strand, within GFF3716 at 21.414 kb on - strand, within GFF3716 at 21.437 kb on + strand, within GFF3716 at 21.437 kb on + strand, within GFF3716 at 21.437 kb on + strand, within GFF3716 at 21.482 kb on + strand, within GFF3716 at 21.483 kb on - strand, within GFF3716 at 21.493 kb on + strand, within GFF3716 at 21.493 kb on + strand, within GFF3716 at 21.494 kb on - strand, within GFF3716 at 21.497 kb on - strand, within GFF3716 at 21.648 kb on - strand, within GFF3716 at 21.664 kb on + strand, within GFF3716 at 21.695 kb on - strand, within GFF3716 at 21.698 kb on + strand, within GFF3716 at 21.698 kb on + strand, within GFF3716 at 21.788 kb on + strand, within GFF3716 at 21.788 kb on + strand, within GFF3716 at 21.792 kb on - strand, within GFF3716 at 21.892 kb on + strand, within GFF3716 at 21.892 kb on + strand, within GFF3716 at 21.897 kb on - strand, within GFF3716
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 19,172 + GFF3712 0.80 +2.2 19,172 + GFF3712 0.80 +2.3 19,173 - GFF3712 0.80 +1.7 19,173 - GFF3712 0.80 +3.4 19,173 - GFF3712 0.80 +2.6 19,315 - +1.0 19,317 - +0.7 19,317 - +3.1 19,402 + +3.5 19,417 - +0.8 19,455 - +0.7 19,455 - +1.5 19,457 + +5.6 19,457 + +0.1 19,463 - -0.2 19,479 + +2.0 19,557 + -3.1 19,557 + +0.2 19,558 - +0.4 19,558 - +0.5 19,565 + -0.0 19,722 + GFF3713 0.29 +2.5 19,722 + GFF3713 0.29 +0.2 19,722 + GFF3713 0.29 -0.3 19,725 + GFF3713 0.30 +0.7 19,725 + GFF3713 0.30 +0.8 19,745 - GFF3713 0.35 -0.4 19,859 - GFF3713 0.63 +0.4 19,859 - GFF3713 0.63 -0.2 19,871 - GFF3713 0.66 -0.1 20,151 + +0.1 20,151 + +0.4 20,152 - -0.1 20,194 + GFF3714 0.10 +1.2 20,195 - GFF3714 0.10 +0.4 20,195 - GFF3714 0.10 -0.9 20,199 + GFF3714 0.11 +0.0 20,397 - GFF3714 0.34 -1.3 20,508 + GFF3714 0.46 -0.3 20,508 + GFF3714 0.46 +0.2 20,632 - GFF3714 0.61 -0.3 20,672 + GFF3714 0.65 -0.1 20,673 - GFF3714 0.65 -0.0 20,673 - GFF3714 0.65 +1.1 20,677 - GFF3714 0.66 +0.2 20,677 - GFF3714 0.66 +0.4 20,677 - GFF3714 0.66 -0.1 20,677 - GFF3714 0.66 +1.0 20,677 - GFF3714 0.66 +0.0 20,677 - GFF3714 0.66 -0.3 20,688 + GFF3714 0.67 +0.6 20,703 + GFF3714 0.69 -0.4 20,724 + GFF3714 0.71 -0.1 20,730 + GFF3714 0.72 +2.0 20,731 - GFF3714 0.72 +0.4 20,731 - GFF3714 0.72 +0.4 20,779 - GFF3714 0.78 +1.4 20,835 - GFF3714 0.84 +0.6 20,945 - +0.2 21,002 - -0.9 21,002 - -0.7 21,002 - +0.1 21,007 - -0.4 21,070 + GFF3715 0.21 -0.4 21,162 - GFF3715 0.63 +0.5 21,259 + -0.5 21,413 + GFF3716 0.18 +1.2 21,414 - GFF3716 0.18 -0.4 21,437 + GFF3716 0.21 -0.7 21,437 + GFF3716 0.21 +0.4 21,437 + GFF3716 0.21 -1.9 21,482 + GFF3716 0.25 +2.3 21,483 - GFF3716 0.25 -0.6 21,493 + GFF3716 0.26 +0.5 21,493 + GFF3716 0.26 +0.5 21,494 - GFF3716 0.26 -0.2 21,497 - GFF3716 0.27 -0.2 21,648 - GFF3716 0.42 -0.1 21,664 + GFF3716 0.44 -0.3 21,695 - GFF3716 0.47 -0.1 21,698 + GFF3716 0.48 +0.0 21,698 + GFF3716 0.48 +0.4 21,788 + GFF3716 0.57 +0.2 21,788 + GFF3716 0.57 +0.0 21,792 - GFF3716 0.57 -1.2 21,892 + GFF3716 0.68 -0.8 21,892 + GFF3716 0.68 -0.8 21,897 - GFF3716 0.68 +0.3
Or see this region's nucleotide sequence