Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3600 and GFF3601 overlap by 4 nucleotides GFF3601 and GFF3602 overlap by 11 nucleotides GFF3602 and GFF3603 are separated by 57 nucleotides GFF3603 and GFF3604 overlap by 16 nucleotides
GFF3600 - FIG029138: Type III secretion protein, at 353,097 to 353,645
GFF3600
GFF3601 - Type III secretion protein SsaK, at 353,642 to 354,316
GFF3601
GFF3602 - Type III secretion cytoplasmic protein (YscL), at 354,306 to 355,298
GFF3602
GFF3603 - Secretion system apparatus protein SsaM, at 355,356 to 355,724
GFF3603
GFF3604 - Type III secretion inner membrane channel protein (LcrD,HrcV,EscV,SsaV), at 355,709 to 357,754
GFF3604
Position (kb) 354
355
356 Strain fitness (log2 ratio) -2
-1
0
1
2 at 353.467 kb on + strand, within GFF3600 at 353.937 kb on + strand, within GFF3601 at 354.003 kb on + strand, within GFF3601 at 354.053 kb on - strand, within GFF3601 at 354.277 kb on - strand at 354.387 kb on - strand at 354.403 kb on + strand at 354.644 kb on + strand, within GFF3602 at 354.792 kb on - strand, within GFF3602 at 354.850 kb on + strand, within GFF3602 at 354.906 kb on - strand, within GFF3602 at 354.986 kb on - strand, within GFF3602 at 355.203 kb on - strand at 355.471 kb on - strand, within GFF3603 at 355.669 kb on + strand, within GFF3603 at 355.778 kb on - strand at 355.962 kb on + strand, within GFF3604 at 356.056 kb on - strand, within GFF3604 at 356.086 kb on - strand, within GFF3604
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 353,467 + GFF3600 0.67 -1.9 353,937 + GFF3601 0.44 +0.2 354,003 + GFF3601 0.53 -1.5 354,053 - GFF3601 0.61 -2.5 354,277 - +0.7 354,387 - +0.9 354,403 + +0.9 354,644 + GFF3602 0.34 -0.0 354,792 - GFF3602 0.49 -0.9 354,850 + GFF3602 0.55 -0.6 354,906 - GFF3602 0.60 -0.3 354,986 - GFF3602 0.68 -0.9 355,203 - -0.3 355,471 - GFF3603 0.31 -1.6 355,669 + GFF3603 0.85 +0.8 355,778 - +0.5 355,962 + GFF3604 0.12 +0.0 356,056 - GFF3604 0.17 +1.6 356,086 - GFF3604 0.18 +2.1
Or see this region's nucleotide sequence