Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3495 and GFF3496 overlap by 8 nucleotides GFF3496 and GFF3497 are separated by 335 nucleotides GFF3497 and GFF3498 are separated by 74 nucleotides
GFF3495 - Succinylarginine dihydrolase (EC 3.5.3.23), at 247,970 to 249,313
GFF3495
GFF3496 - Succinylglutamate desuccinylase (EC 3.5.1.96), at 249,306 to 250,274
GFF3496
GFF3497 - Periplasmic protein related to spheroblast formation, at 250,610 to 251,095
GFF3497
GFF3498 - Excinuclease cho (excinuclease ABC alternative C subunit), at 251,170 to 252,078
GFF3498
Position (kb)
249
250
251 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 248.365 kb on - strand, within GFF3495 at 248.425 kb on - strand, within GFF3495 at 248.877 kb on - strand, within GFF3495 at 248.884 kb on + strand, within GFF3495 at 248.925 kb on + strand, within GFF3495 at 249.038 kb on - strand, within GFF3495 at 249.171 kb on - strand, within GFF3495 at 249.173 kb on + strand, within GFF3495 at 249.331 kb on + strand at 249.442 kb on + strand, within GFF3496 at 249.686 kb on - strand, within GFF3496 at 249.926 kb on + strand, within GFF3496 at 249.985 kb on + strand, within GFF3496 at 250.104 kb on + strand, within GFF3496 at 250.104 kb on + strand, within GFF3496 at 250.105 kb on - strand, within GFF3496 at 250.105 kb on - strand, within GFF3496 at 250.105 kb on - strand, within GFF3496 at 250.106 kb on + strand, within GFF3496 at 250.276 kb on - strand at 250.305 kb on - strand at 250.629 kb on + strand at 250.730 kb on - strand, within GFF3497 at 250.813 kb on + strand, within GFF3497 at 250.967 kb on - strand, within GFF3497 at 251.023 kb on + strand, within GFF3497 at 251.210 kb on + strand at 251.224 kb on + strand at 251.244 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 248,365 - GFF3495 0.29 -0.5 248,425 - GFF3495 0.34 +0.2 248,877 - GFF3495 0.67 +0.2 248,884 + GFF3495 0.68 +0.2 248,925 + GFF3495 0.71 -1.8 249,038 - GFF3495 0.79 +0.2 249,171 - GFF3495 0.89 -3.2 249,173 + GFF3495 0.90 -1.2 249,331 + -2.1 249,442 + GFF3496 0.14 -0.5 249,686 - GFF3496 0.39 -0.9 249,926 + GFF3496 0.64 +0.3 249,985 + GFF3496 0.70 -1.4 250,104 + GFF3496 0.82 -1.5 250,104 + GFF3496 0.82 +0.9 250,105 - GFF3496 0.82 -0.5 250,105 - GFF3496 0.82 -0.5 250,105 - GFF3496 0.82 -0.8 250,106 + GFF3496 0.83 -0.5 250,276 - -2.3 250,305 - +0.5 250,629 + +1.8 250,730 - GFF3497 0.25 +0.3 250,813 + GFF3497 0.42 +0.0 250,967 - GFF3497 0.73 +0.1 251,023 + GFF3497 0.85 -0.9 251,210 + +1.7 251,224 + -0.6 251,244 + +1.1
Or see this region's nucleotide sequence