Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3467 and GFF3468 are separated by 1 nucleotides GFF3468 and GFF3469 are separated by 30 nucleotides GFF3469 and GFF3470 overlap by 4 nucleotides GFF3470 and GFF3471 are separated by 40 nucleotides GFF3471 and GFF3472 are separated by 640 nucleotides
GFF3467 - Putative inner membrane protein, at 219,014 to 219,460
GFF3467
GFF3468 - hypothetical protein, at 219,462 to 219,599
GFF3468
GFF3469 - hypothetical protein, at 219,630 to 219,773
GFF3469
GFF3470 - Uncharacterized protein YeaK, at 219,770 to 220,297
GFF3470
GFF3471 - Putative two-component response regulator and GGDEF family protein YeaJ, at 220,338 to 221,831
GFF3471
GFF3472 - UPF0229 protein YeaH, at 222,472 to 223,758
GFF3472
Position (kb) 220
221
222 Strain fitness (log2 ratio) -1
0
1
2 at 219.365 kb on - strand, within GFF3467 at 219.618 kb on + strand at 219.665 kb on + strand, within GFF3469 at 219.666 kb on - strand, within GFF3469 at 219.666 kb on - strand, within GFF3469 at 219.666 kb on - strand, within GFF3469 at 219.702 kb on - strand, within GFF3469 at 219.834 kb on + strand, within GFF3470 at 219.851 kb on - strand, within GFF3470 at 220.164 kb on - strand, within GFF3470 at 220.340 kb on - strand at 220.431 kb on - strand at 220.604 kb on + strand, within GFF3471 at 221.244 kb on + strand, within GFF3471 at 221.480 kb on + strand, within GFF3471 at 221.514 kb on + strand, within GFF3471 at 221.719 kb on + strand at 221.720 kb on - strand at 222.264 kb on + strand at 222.334 kb on + strand at 222.416 kb on - strand at 222.474 kb on - strand at 222.602 kb on - strand, within GFF3472 at 222.642 kb on - strand, within GFF3472 at 222.644 kb on + strand, within GFF3472 at 222.711 kb on + strand, within GFF3472 at 222.737 kb on + strand, within GFF3472 at 222.738 kb on - strand, within GFF3472
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 219,365 - GFF3467 0.79 -0.4 219,618 + -1.4 219,665 + GFF3469 0.24 -1.7 219,666 - GFF3469 0.25 -0.9 219,666 - GFF3469 0.25 -0.9 219,666 - GFF3469 0.25 -0.5 219,702 - GFF3469 0.50 +1.5 219,834 + GFF3470 0.12 +0.3 219,851 - GFF3470 0.15 -0.1 220,164 - GFF3470 0.75 -0.3 220,340 - +0.1 220,431 - +0.5 220,604 + GFF3471 0.18 +0.5 221,244 + GFF3471 0.61 +0.0 221,480 + GFF3471 0.76 +2.7 221,514 + GFF3471 0.79 +1.0 221,719 + +2.0 221,720 - -0.0 222,264 + +0.7 222,334 + +0.0 222,416 - -0.7 222,474 - +0.2 222,602 - GFF3472 0.10 +0.5 222,642 - GFF3472 0.13 +1.4 222,644 + GFF3472 0.13 -0.2 222,711 + GFF3472 0.19 -0.1 222,737 + GFF3472 0.21 -0.4 222,738 - GFF3472 0.21 -0.9
Or see this region's nucleotide sequence