Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF332 and GFF333 are separated by 7 nucleotides GFF333 and GFF334 are separated by 98 nucleotides GFF334 and GFF335 are separated by 266 nucleotides
GFF332 - Mg(2+) transport ATPase protein C, at 47,913 to 48,590
GFF332
GFF333 - hypothetical protein, at 48,598 to 48,711
GFF333
GFF334 - Mg(2+) transport ATPase, P-type (EC 3.6.3.2), at 48,810 to 51,536
GFF334
GFF335 - Putative inner membrane protein, at 51,803 to 52,330
GFF335
Position (kb)
48
49
50
51
52 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 47.869 kb on - strand at 47.993 kb on + strand, within GFF332 at 47.993 kb on + strand, within GFF332 at 47.994 kb on - strand, within GFF332 at 48.302 kb on - strand, within GFF332 at 48.341 kb on + strand, within GFF332 at 48.436 kb on + strand, within GFF332 at 48.437 kb on - strand, within GFF332 at 48.626 kb on + strand, within GFF333 at 48.964 kb on + strand at 49.075 kb on - strand at 49.199 kb on - strand, within GFF334 at 49.226 kb on + strand, within GFF334 at 49.437 kb on - strand, within GFF334 at 49.472 kb on + strand, within GFF334 at 49.490 kb on + strand, within GFF334 at 49.567 kb on + strand, within GFF334 at 49.713 kb on - strand, within GFF334 at 49.960 kb on + strand, within GFF334 at 49.974 kb on - strand, within GFF334 at 50.008 kb on + strand, within GFF334 at 50.048 kb on + strand, within GFF334 at 50.048 kb on + strand, within GFF334 at 50.049 kb on - strand, within GFF334 at 50.170 kb on + strand, within GFF334 at 50.261 kb on - strand, within GFF334 at 50.261 kb on - strand, within GFF334 at 50.344 kb on - strand, within GFF334 at 50.365 kb on + strand, within GFF334 at 50.384 kb on + strand, within GFF334 at 50.384 kb on + strand, within GFF334 at 50.384 kb on + strand, within GFF334 at 50.384 kb on + strand, within GFF334 at 50.385 kb on - strand, within GFF334 at 50.416 kb on + strand, within GFF334 at 50.442 kb on - strand, within GFF334 at 50.847 kb on - strand, within GFF334 at 50.850 kb on - strand, within GFF334 at 50.924 kb on + strand, within GFF334 at 50.925 kb on - strand, within GFF334 at 51.045 kb on + strand, within GFF334 at 51.141 kb on - strand, within GFF334 at 51.294 kb on - strand at 51.320 kb on + strand at 51.349 kb on + strand at 51.384 kb on + strand at 51.384 kb on + strand at 51.386 kb on + strand at 51.475 kb on - strand at 51.535 kb on - strand at 51.592 kb on + strand at 51.625 kb on + strand at 51.626 kb on - strand at 51.778 kb on - strand at 51.896 kb on - strand, within GFF335 at 52.000 kb on + strand, within GFF335 at 52.073 kb on - strand, within GFF335 at 52.073 kb on - strand, within GFF335 at 52.076 kb on + strand, within GFF335 at 52.076 kb on + strand, within GFF335 at 52.076 kb on + strand, within GFF335 at 52.077 kb on - strand, within GFF335 at 52.077 kb on - strand, within GFF335 at 52.122 kb on - strand, within GFF335 at 52.131 kb on + strand, within GFF335 at 52.131 kb on + strand, within GFF335 at 52.211 kb on - strand, within GFF335 at 52.211 kb on - strand, within GFF335 at 52.225 kb on - strand, within GFF335 at 52.242 kb on - strand, within GFF335 at 52.265 kb on - strand, within GFF335 at 52.329 kb on - strand at 52.526 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 47,869 - +0.2 47,993 + GFF332 0.12 +0.6 47,993 + GFF332 0.12 +0.7 47,994 - GFF332 0.12 +0.1 48,302 - GFF332 0.57 -0.2 48,341 + GFF332 0.63 +0.9 48,436 + GFF332 0.77 +0.4 48,437 - GFF332 0.77 +0.7 48,626 + GFF333 0.25 +0.5 48,964 + -1.3 49,075 - +0.0 49,199 - GFF334 0.14 -1.0 49,226 + GFF334 0.15 -1.3 49,437 - GFF334 0.23 -0.7 49,472 + GFF334 0.24 -0.1 49,490 + GFF334 0.25 +0.3 49,567 + GFF334 0.28 -0.0 49,713 - GFF334 0.33 +1.4 49,960 + GFF334 0.42 +0.1 49,974 - GFF334 0.43 -1.2 50,008 + GFF334 0.44 -0.1 50,048 + GFF334 0.45 +0.1 50,048 + GFF334 0.45 +0.1 50,049 - GFF334 0.45 +1.1 50,170 + GFF334 0.50 -0.3 50,261 - GFF334 0.53 -0.5 50,261 - GFF334 0.53 -2.2 50,344 - GFF334 0.56 -0.9 50,365 + GFF334 0.57 -0.6 50,384 + GFF334 0.58 -0.6 50,384 + GFF334 0.58 -0.6 50,384 + GFF334 0.58 +0.6 50,384 + GFF334 0.58 -0.2 50,385 - GFF334 0.58 -0.1 50,416 + GFF334 0.59 +0.1 50,442 - GFF334 0.60 -0.4 50,847 - GFF334 0.75 -1.7 50,850 - GFF334 0.75 -0.2 50,924 + GFF334 0.78 +0.2 50,925 - GFF334 0.78 +0.1 51,045 + GFF334 0.82 -0.4 51,141 - GFF334 0.85 +0.0 51,294 - -1.4 51,320 + -0.8 51,349 + +1.5 51,384 + +1.4 51,384 + -0.1 51,386 + +1.1 51,475 - -2.6 51,535 - -0.2 51,592 + -1.0 51,625 + +0.3 51,626 - +0.0 51,778 - -0.8 51,896 - GFF335 0.18 +0.4 52,000 + GFF335 0.37 +0.1 52,073 - GFF335 0.51 +0.3 52,073 - GFF335 0.51 -1.0 52,076 + GFF335 0.52 +0.5 52,076 + GFF335 0.52 -0.3 52,076 + GFF335 0.52 -0.4 52,077 - GFF335 0.52 -0.4 52,077 - GFF335 0.52 -2.9 52,122 - GFF335 0.60 -0.5 52,131 + GFF335 0.62 +0.1 52,131 + GFF335 0.62 -1.0 52,211 - GFF335 0.77 +0.7 52,211 - GFF335 0.77 +1.9 52,225 - GFF335 0.80 +0.1 52,242 - GFF335 0.83 -1.7 52,265 - GFF335 0.88 -0.8 52,329 - +0.5 52,526 - -0.0
Or see this region's nucleotide sequence