Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3092 and GFF3093 are separated by 404 nucleotides GFF3093 and GFF3094 are separated by 110 nucleotides
GFF3092 - Hexuronate transporter, at 99,554 to 100,858
GFF3092
GFF3093 - Mannonate dehydratase (EC 4.2.1.8), at 101,263 to 102,447
GFF3093
GFF3094 - D-mannonate oxidoreductase (EC 1.1.1.57), at 102,558 to 104,030
GFF3094
Position (kb)
101
102
103 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 100.293 kb on + strand, within GFF3092 at 100.373 kb on - strand, within GFF3092 at 100.566 kb on - strand, within GFF3092 at 100.625 kb on - strand, within GFF3092 at 100.663 kb on - strand, within GFF3092 at 100.775 kb on - strand at 100.794 kb on + strand at 100.795 kb on - strand at 100.861 kb on + strand at 100.996 kb on + strand at 101.263 kb on - strand at 101.292 kb on + strand at 101.456 kb on + strand, within GFF3093 at 101.456 kb on + strand, within GFF3093 at 101.456 kb on + strand, within GFF3093 at 101.456 kb on + strand, within GFF3093 at 101.457 kb on - strand, within GFF3093 at 101.532 kb on + strand, within GFF3093 at 101.533 kb on - strand, within GFF3093 at 101.551 kb on - strand, within GFF3093 at 101.555 kb on - strand, within GFF3093 at 101.556 kb on + strand, within GFF3093 at 101.568 kb on + strand, within GFF3093 at 101.574 kb on + strand, within GFF3093 at 101.599 kb on - strand, within GFF3093 at 101.599 kb on - strand, within GFF3093 at 101.611 kb on + strand, within GFF3093 at 101.611 kb on + strand, within GFF3093 at 101.611 kb on + strand, within GFF3093 at 101.611 kb on + strand, within GFF3093 at 101.615 kb on + strand, within GFF3093 at 101.616 kb on - strand, within GFF3093 at 101.616 kb on - strand, within GFF3093 at 101.616 kb on - strand, within GFF3093 at 101.710 kb on + strand, within GFF3093 at 101.713 kb on - strand, within GFF3093 at 101.824 kb on + strand, within GFF3093 at 101.827 kb on - strand, within GFF3093 at 101.827 kb on - strand, within GFF3093 at 101.860 kb on + strand, within GFF3093 at 101.863 kb on - strand, within GFF3093 at 101.868 kb on + strand, within GFF3093 at 102.048 kb on + strand, within GFF3093 at 102.048 kb on + strand, within GFF3093 at 102.049 kb on - strand, within GFF3093 at 102.049 kb on - strand, within GFF3093 at 102.117 kb on + strand, within GFF3093 at 102.117 kb on + strand, within GFF3093 at 102.145 kb on + strand, within GFF3093 at 102.146 kb on - strand, within GFF3093 at 102.150 kb on + strand, within GFF3093 at 102.228 kb on + strand, within GFF3093 at 102.228 kb on + strand, within GFF3093 at 102.228 kb on + strand, within GFF3093 at 102.228 kb on + strand, within GFF3093 at 102.229 kb on - strand, within GFF3093 at 102.232 kb on + strand, within GFF3093 at 102.232 kb on + strand, within GFF3093 at 102.232 kb on + strand, within GFF3093 at 102.232 kb on + strand, within GFF3093 at 102.233 kb on - strand, within GFF3093 at 102.233 kb on - strand, within GFF3093 at 102.233 kb on - strand, within GFF3093 at 102.233 kb on - strand, within GFF3093 at 102.233 kb on - strand, within GFF3093 at 102.488 kb on - strand at 102.500 kb on - strand at 102.633 kb on - strand at 102.633 kb on - strand at 102.795 kb on + strand, within GFF3094 at 102.795 kb on + strand, within GFF3094 at 103.066 kb on + strand, within GFF3094 at 103.271 kb on - strand, within GFF3094 at 103.271 kb on - strand, within GFF3094 at 103.300 kb on - strand, within GFF3094
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 100,293 + GFF3092 0.57 -0.2 100,373 - GFF3092 0.63 -0.3 100,566 - GFF3092 0.78 -0.2 100,625 - GFF3092 0.82 -0.0 100,663 - GFF3092 0.85 -0.4 100,775 - +0.7 100,794 + +0.5 100,795 - +1.4 100,861 + -0.4 100,996 + +1.1 101,263 - -0.0 101,292 + +0.4 101,456 + GFF3093 0.16 -0.0 101,456 + GFF3093 0.16 -0.1 101,456 + GFF3093 0.16 +0.3 101,456 + GFF3093 0.16 -0.1 101,457 - GFF3093 0.16 -1.0 101,532 + GFF3093 0.23 -0.4 101,533 - GFF3093 0.23 -0.6 101,551 - GFF3093 0.24 +0.1 101,555 - GFF3093 0.25 -0.1 101,556 + GFF3093 0.25 -0.2 101,568 + GFF3093 0.26 -1.0 101,574 + GFF3093 0.26 +0.7 101,599 - GFF3093 0.28 +0.0 101,599 - GFF3093 0.28 -0.7 101,611 + GFF3093 0.29 -1.2 101,611 + GFF3093 0.29 -1.2 101,611 + GFF3093 0.29 -0.6 101,611 + GFF3093 0.29 -0.1 101,615 + GFF3093 0.30 +0.3 101,616 - GFF3093 0.30 -0.1 101,616 - GFF3093 0.30 -0.2 101,616 - GFF3093 0.30 -1.0 101,710 + GFF3093 0.38 +0.5 101,713 - GFF3093 0.38 +0.0 101,824 + GFF3093 0.47 +0.1 101,827 - GFF3093 0.48 -0.3 101,827 - GFF3093 0.48 -3.2 101,860 + GFF3093 0.50 -0.8 101,863 - GFF3093 0.51 +2.7 101,868 + GFF3093 0.51 -0.3 102,048 + GFF3093 0.66 -0.4 102,048 + GFF3093 0.66 +0.1 102,049 - GFF3093 0.66 -0.3 102,049 - GFF3093 0.66 +0.6 102,117 + GFF3093 0.72 -0.9 102,117 + GFF3093 0.72 +1.1 102,145 + GFF3093 0.74 +0.1 102,146 - GFF3093 0.75 -0.6 102,150 + GFF3093 0.75 -1.6 102,228 + GFF3093 0.81 -1.2 102,228 + GFF3093 0.81 -0.0 102,228 + GFF3093 0.81 -1.2 102,228 + GFF3093 0.81 +0.1 102,229 - GFF3093 0.82 -0.6 102,232 + GFF3093 0.82 -0.8 102,232 + GFF3093 0.82 -0.4 102,232 + GFF3093 0.82 +0.2 102,232 + GFF3093 0.82 -1.6 102,233 - GFF3093 0.82 -3.4 102,233 - GFF3093 0.82 -0.8 102,233 - GFF3093 0.82 +0.0 102,233 - GFF3093 0.82 +1.3 102,233 - GFF3093 0.82 -0.6 102,488 - -0.7 102,500 - +0.3 102,633 - -0.2 102,633 - -0.7 102,795 + GFF3094 0.16 +0.1 102,795 + GFF3094 0.16 -0.0 103,066 + GFF3094 0.34 +0.4 103,271 - GFF3094 0.48 +0.6 103,271 - GFF3094 0.48 +0.3 103,300 - GFF3094 0.50 -0.8
Or see this region's nucleotide sequence