Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3085 and GFF3086 are separated by 41 nucleotides GFF3086 and GFF3087 overlap by 20 nucleotides GFF3087 and GFF3088 are separated by 170 nucleotides GFF3088 and GFF3089 are separated by 307 nucleotides GFF3089 and GFF3090 are separated by 262 nucleotides
GFF3085 - Aldehyde dehydrogenase (EC 1.2.1.3), at 94,107 to 95,591
GFF3085
GFF3086 - hypothetical protein, at 95,633 to 95,746
GFF3086
GFF3087 - FIG074102: hypothetical protein, at 95,727 to 95,936
GFF3087
GFF3088 - Putative cytoplasmic protein USSDB7A, at 96,107 to 96,592
GFF3088
GFF3089 - FIG01045853: hypothetical protein, at 96,900 to 97,055
GFF3089
GFF3090 - Putative xylanase, at 97,318 to 98,241
GFF3090
Position (kb) 96
97 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 95.199 kb on - strand, within GFF3085 at 95.409 kb on - strand, within GFF3085 at 95.409 kb on - strand, within GFF3085 at 95.415 kb on - strand, within GFF3085 at 95.415 kb on - strand, within GFF3085 at 95.522 kb on - strand at 95.557 kb on - strand at 95.567 kb on + strand at 95.695 kb on - strand, within GFF3086 at 95.726 kb on - strand, within GFF3086 at 95.739 kb on - strand at 95.750 kb on - strand, within GFF3087 at 95.755 kb on - strand, within GFF3087 at 95.788 kb on - strand, within GFF3087 at 95.788 kb on - strand, within GFF3087 at 96.021 kb on + strand at 96.294 kb on + strand, within GFF3088 at 96.294 kb on + strand, within GFF3088 at 96.359 kb on + strand, within GFF3088 at 96.378 kb on + strand, within GFF3088 at 96.379 kb on - strand, within GFF3088 at 96.379 kb on - strand, within GFF3088 at 96.522 kb on - strand, within GFF3088 at 96.562 kb on + strand at 96.669 kb on - strand at 96.777 kb on - strand at 97.467 kb on + strand, within GFF3090 at 97.468 kb on - strand, within GFF3090 at 97.484 kb on - strand, within GFF3090 at 97.484 kb on - strand, within GFF3090 at 97.497 kb on + strand, within GFF3090 at 97.497 kb on + strand, within GFF3090 at 97.544 kb on - strand, within GFF3090 at 97.566 kb on - strand, within GFF3090 at 97.572 kb on - strand, within GFF3090
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 95,199 - GFF3085 0.74 +0.3 95,409 - GFF3085 0.88 -1.2 95,409 - GFF3085 0.88 -0.3 95,415 - GFF3085 0.88 -0.1 95,415 - GFF3085 0.88 +0.7 95,522 - +0.6 95,557 - -0.3 95,567 + +0.6 95,695 - GFF3086 0.54 +0.4 95,726 - GFF3086 0.82 +1.3 95,739 - -0.1 95,750 - GFF3087 0.11 -1.6 95,755 - GFF3087 0.13 -0.2 95,788 - GFF3087 0.29 -0.5 95,788 - GFF3087 0.29 +0.2 96,021 + +0.2 96,294 + GFF3088 0.38 +0.1 96,294 + GFF3088 0.38 +3.4 96,359 + GFF3088 0.52 -0.5 96,378 + GFF3088 0.56 -0.8 96,379 - GFF3088 0.56 -0.3 96,379 - GFF3088 0.56 -0.3 96,522 - GFF3088 0.85 -1.8 96,562 + +0.7 96,669 - +0.8 96,777 - -0.3 97,467 + GFF3090 0.16 +2.0 97,468 - GFF3090 0.16 +0.4 97,484 - GFF3090 0.18 -0.1 97,484 - GFF3090 0.18 +1.2 97,497 + GFF3090 0.19 +0.8 97,497 + GFF3090 0.19 +0.2 97,544 - GFF3090 0.24 -0.3 97,566 - GFF3090 0.27 -0.2 97,572 - GFF3090 0.27 -0.2
Or see this region's nucleotide sequence