Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3049 and GFF3050 are separated by 215 nucleotides GFF3050 and GFF3051 overlap by 1 nucleotides GFF3051 and GFF3052 are separated by 46 nucleotides GFF3052 and GFF3053 are separated by 131 nucleotides
GFF3049 - Glutathione synthetase (EC 6.3.2.3), at 62,074 to 63,021
GFF3049
GFF3050 - UPF0301 protein YqgE, at 63,237 to 63,800
GFF3050
GFF3051 - Putative Holliday junction resolvase YggF, at 63,800 to 64,216
GFF3051
GFF3052 - Transcriptional regulator, IclR family, at 64,263 to 64,949
GFF3052
GFF3053 - Twitching motility protein PilT, at 65,081 to 66,061
GFF3053
Position (kb) 63
64
65 Strain fitness (log2 ratio) -2
-1
0
1 at 63.066 kb on - strand at 63.117 kb on + strand at 63.158 kb on + strand at 63.160 kb on + strand at 63.161 kb on - strand at 63.161 kb on - strand at 63.190 kb on + strand at 63.311 kb on + strand, within GFF3050 at 63.624 kb on + strand, within GFF3050 at 64.214 kb on + strand at 64.215 kb on - strand at 64.302 kb on + strand at 64.388 kb on + strand, within GFF3052 at 64.419 kb on + strand, within GFF3052 at 64.602 kb on - strand, within GFF3052 at 64.610 kb on - strand, within GFF3052 at 64.613 kb on + strand, within GFF3052 at 64.613 kb on + strand, within GFF3052 at 64.615 kb on + strand, within GFF3052 at 64.616 kb on - strand, within GFF3052 at 64.619 kb on + strand, within GFF3052 at 64.620 kb on - strand, within GFF3052 at 64.636 kb on - strand, within GFF3052 at 64.636 kb on - strand, within GFF3052 at 64.644 kb on + strand, within GFF3052 at 64.763 kb on - strand, within GFF3052 at 64.835 kb on + strand, within GFF3052 at 64.836 kb on - strand, within GFF3052 at 64.838 kb on + strand, within GFF3052 at 64.839 kb on - strand, within GFF3052 at 64.842 kb on - strand, within GFF3052 at 64.842 kb on - strand, within GFF3052 at 64.842 kb on - strand, within GFF3052 at 64.897 kb on + strand at 64.898 kb on - strand at 64.920 kb on + strand at 64.953 kb on + strand at 65.055 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 63,066 - +0.2 63,117 + -1.8 63,158 + +0.3 63,160 + +0.1 63,161 - -1.2 63,161 - -0.5 63,190 + -2.6 63,311 + GFF3050 0.13 -0.1 63,624 + GFF3050 0.69 -1.0 64,214 + +0.2 64,215 - -1.3 64,302 + +0.4 64,388 + GFF3052 0.18 +0.4 64,419 + GFF3052 0.23 +0.1 64,602 - GFF3052 0.49 +0.4 64,610 - GFF3052 0.51 +0.4 64,613 + GFF3052 0.51 +0.1 64,613 + GFF3052 0.51 +0.4 64,615 + GFF3052 0.51 +0.1 64,616 - GFF3052 0.51 -0.2 64,619 + GFF3052 0.52 +0.2 64,620 - GFF3052 0.52 +0.9 64,636 - GFF3052 0.54 +0.4 64,636 - GFF3052 0.54 -0.2 64,644 + GFF3052 0.55 +1.6 64,763 - GFF3052 0.73 -0.1 64,835 + GFF3052 0.83 -0.5 64,836 - GFF3052 0.83 -0.4 64,838 + GFF3052 0.84 -0.1 64,839 - GFF3052 0.84 +1.0 64,842 - GFF3052 0.84 -0.3 64,842 - GFF3052 0.84 -0.2 64,842 - GFF3052 0.84 -0.5 64,897 + +0.7 64,898 - -0.5 64,920 + -0.6 64,953 + +0.3 65,055 - -0.2
Or see this region's nucleotide sequence