Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2957 and GFF2958 are separated by 206 nucleotides GFF2958 and GFF2959 are separated by 173 nucleotides
GFF2957 - Glutamate synthase [NADPH] small chain (EC 1.4.1.13), at 40,469 to 41,887
GFF2957
GFF2958 - Putative uncharacterized protein YhcG, at 42,094 to 43,197
GFF2958
GFF2959 - Cytosine permease, at 43,371 to 44,567
GFF2959
Position (kb) 42
43
44 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 41.113 kb on + strand, within GFF2957 at 41.136 kb on + strand, within GFF2957 at 41.173 kb on - strand, within GFF2957 at 41.311 kb on + strand, within GFF2957 at 41.312 kb on + strand, within GFF2957 at 41.312 kb on + strand, within GFF2957 at 41.313 kb on - strand, within GFF2957 at 41.313 kb on - strand, within GFF2957 at 41.313 kb on - strand, within GFF2957 at 41.410 kb on - strand, within GFF2957 at 41.410 kb on - strand, within GFF2957 at 41.500 kb on + strand, within GFF2957 at 41.501 kb on - strand, within GFF2957 at 41.756 kb on - strand at 41.873 kb on + strand at 41.951 kb on + strand at 41.995 kb on + strand at 41.995 kb on + strand at 41.995 kb on + strand at 41.996 kb on - strand at 41.996 kb on - strand at 41.996 kb on - strand at 42.131 kb on - strand at 42.475 kb on + strand, within GFF2958 at 42.827 kb on + strand, within GFF2958 at 42.827 kb on + strand, within GFF2958 at 42.827 kb on + strand, within GFF2958 at 42.942 kb on + strand, within GFF2958 at 42.942 kb on + strand, within GFF2958 at 42.942 kb on + strand, within GFF2958 at 42.943 kb on - strand, within GFF2958 at 42.943 kb on - strand, within GFF2958 at 42.943 kb on - strand, within GFF2958 at 42.943 kb on - strand, within GFF2958 at 42.943 kb on - strand, within GFF2958 at 42.946 kb on + strand, within GFF2958 at 43.085 kb on - strand, within GFF2958 at 43.128 kb on + strand at 43.129 kb on - strand at 43.219 kb on - strand at 43.332 kb on + strand at 43.422 kb on - strand at 43.459 kb on - strand at 43.459 kb on - strand at 43.509 kb on + strand, within GFF2959 at 43.509 kb on + strand, within GFF2959 at 43.528 kb on - strand, within GFF2959 at 43.572 kb on + strand, within GFF2959 at 43.572 kb on + strand, within GFF2959 at 43.573 kb on - strand, within GFF2959 at 43.677 kb on - strand, within GFF2959 at 43.700 kb on + strand, within GFF2959 at 44.034 kb on - strand, within GFF2959
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 41,113 + GFF2957 0.45 -0.2 41,136 + GFF2957 0.47 +0.0 41,173 - GFF2957 0.50 +0.7 41,311 + GFF2957 0.59 -0.7 41,312 + GFF2957 0.59 +0.9 41,312 + GFF2957 0.59 +0.4 41,313 - GFF2957 0.59 -0.4 41,313 - GFF2957 0.59 +0.4 41,313 - GFF2957 0.59 +0.7 41,410 - GFF2957 0.66 +1.3 41,410 - GFF2957 0.66 -0.3 41,500 + GFF2957 0.73 +0.1 41,501 - GFF2957 0.73 +0.1 41,756 - -2.7 41,873 + +0.4 41,951 + +0.3 41,995 + +0.8 41,995 + +0.7 41,995 + +3.0 41,996 - -0.2 41,996 - -0.3 41,996 - +0.0 42,131 - -1.7 42,475 + GFF2958 0.35 +0.2 42,827 + GFF2958 0.66 -1.7 42,827 + GFF2958 0.66 -1.3 42,827 + GFF2958 0.66 +0.8 42,942 + GFF2958 0.77 +0.0 42,942 + GFF2958 0.77 -0.2 42,942 + GFF2958 0.77 -0.2 42,943 - GFF2958 0.77 -2.5 42,943 - GFF2958 0.77 +0.5 42,943 - GFF2958 0.77 +1.8 42,943 - GFF2958 0.77 -0.9 42,943 - GFF2958 0.77 +1.3 42,946 + GFF2958 0.77 -0.2 43,085 - GFF2958 0.90 -2.2 43,128 + -0.5 43,129 - -0.5 43,219 - +2.5 43,332 + +0.5 43,422 - -0.1 43,459 - -0.3 43,459 - -0.1 43,509 + GFF2959 0.12 -0.7 43,509 + GFF2959 0.12 -1.3 43,528 - GFF2959 0.13 -0.3 43,572 + GFF2959 0.17 +0.1 43,572 + GFF2959 0.17 -0.4 43,573 - GFF2959 0.17 +0.4 43,677 - GFF2959 0.26 +1.2 43,700 + GFF2959 0.27 +0.2 44,034 - GFF2959 0.55 +0.3
Or see this region's nucleotide sequence