Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2879 and GFF2880 overlap by 4 nucleotides GFF2880 and GFF2881 overlap by 4 nucleotides
GFF2879 - Putative ECA polymerase, at 2,714 to 4,072
GFF2879
GFF2880 - 4-alpha-L-fucosyltransferase (EC 2.4.1.-), at 4,069 to 5,148
GFF2880
GFF2881 - WzxE protein, at 5,145 to 6,395
GFF2881
Position (kb) 4
5
6 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 3.415 kb on + strand, within GFF2879 at 4.104 kb on + strand at 4.121 kb on + strand at 4.121 kb on + strand at 4.156 kb on + strand at 4.200 kb on + strand, within GFF2880 at 4.276 kb on + strand, within GFF2880 at 4.464 kb on + strand, within GFF2880 at 4.466 kb on + strand, within GFF2880 at 4.466 kb on + strand, within GFF2880 at 4.466 kb on + strand, within GFF2880 at 4.466 kb on + strand, within GFF2880 at 4.466 kb on + strand, within GFF2880 at 4.466 kb on + strand, within GFF2880 at 4.533 kb on - strand, within GFF2880 at 4.827 kb on + strand, within GFF2880 at 4.969 kb on - strand, within GFF2880 at 5.103 kb on + strand at 5.201 kb on + strand at 5.202 kb on - strand at 5.203 kb on + strand at 5.203 kb on + strand at 5.213 kb on + strand at 5.215 kb on + strand at 5.215 kb on + strand at 5.215 kb on + strand at 5.215 kb on + strand at 5.216 kb on - strand at 5.382 kb on + strand, within GFF2881 at 5.605 kb on + strand, within GFF2881 at 5.605 kb on + strand, within GFF2881 at 5.618 kb on + strand, within GFF2881 at 5.625 kb on + strand, within GFF2881 at 5.657 kb on + strand, within GFF2881 at 5.658 kb on - strand, within GFF2881 at 5.658 kb on - strand, within GFF2881 at 5.659 kb on + strand, within GFF2881 at 5.692 kb on + strand, within GFF2881 at 5.817 kb on + strand, within GFF2881 at 5.817 kb on + strand, within GFF2881 at 5.817 kb on + strand, within GFF2881 at 5.818 kb on - strand, within GFF2881 at 5.818 kb on - strand, within GFF2881 at 5.820 kb on + strand, within GFF2881 at 5.822 kb on + strand, within GFF2881 at 5.836 kb on - strand, within GFF2881 at 5.946 kb on + strand, within GFF2881 at 6.052 kb on + strand, within GFF2881 at 6.080 kb on + strand, within GFF2881 at 6.122 kb on + strand, within GFF2881 at 6.123 kb on - strand, within GFF2881 at 6.135 kb on + strand, within GFF2881
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 3,415 + GFF2879 0.52 -0.5 4,104 + +0.7 4,121 + -3.7 4,121 + -0.5 4,156 + -1.9 4,200 + GFF2880 0.12 -1.7 4,276 + GFF2880 0.19 -0.8 4,464 + GFF2880 0.37 -0.3 4,466 + GFF2880 0.37 +0.6 4,466 + GFF2880 0.37 -1.5 4,466 + GFF2880 0.37 -0.3 4,466 + GFF2880 0.37 -1.1 4,466 + GFF2880 0.37 -1.8 4,466 + GFF2880 0.37 -0.5 4,533 - GFF2880 0.43 +0.4 4,827 + GFF2880 0.70 -0.3 4,969 - GFF2880 0.83 -2.8 5,103 + -0.5 5,201 + -0.3 5,202 - +0.8 5,203 + -0.5 5,203 + -3.0 5,213 + -2.5 5,215 + -1.1 5,215 + -1.1 5,215 + +0.6 5,215 + -1.5 5,216 - -3.3 5,382 + GFF2881 0.19 -1.1 5,605 + GFF2881 0.37 -1.1 5,605 + GFF2881 0.37 -1.7 5,618 + GFF2881 0.38 -2.1 5,625 + GFF2881 0.38 -1.7 5,657 + GFF2881 0.41 +0.9 5,658 - GFF2881 0.41 -1.7 5,658 - GFF2881 0.41 -1.3 5,659 + GFF2881 0.41 +0.5 5,692 + GFF2881 0.44 +0.7 5,817 + GFF2881 0.54 -2.9 5,817 + GFF2881 0.54 -1.3 5,817 + GFF2881 0.54 -1.0 5,818 - GFF2881 0.54 -1.9 5,818 - GFF2881 0.54 -1.1 5,820 + GFF2881 0.54 -0.8 5,822 + GFF2881 0.54 -1.9 5,836 - GFF2881 0.55 +0.2 5,946 + GFF2881 0.64 -2.5 6,052 + GFF2881 0.73 -2.3 6,080 + GFF2881 0.75 -0.5 6,122 + GFF2881 0.78 -1.2 6,123 - GFF2881 0.78 -1.3 6,135 + GFF2881 0.79 -1.1
Or see this region's nucleotide sequence