Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2566 and GFF2567 are separated by 364 nucleotides GFF2567 and GFF2568 are separated by 91 nucleotides
GFF2566 - Transcriptional regulator, TetR family, at 84,032 to 84,610
GFF2566
GFF2567 - Nitrate/nitrite transporter, at 84,975 to 86,213
GFF2567
GFF2568 - Respiratory nitrate reductase alpha chain (EC 1.7.99.4), at 86,305 to 90,045
GFF2568
Position (kb) 84
85
86
87 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 84.038 kb on + strand at 84.384 kb on - strand, within GFF2566 at 84.384 kb on - strand, within GFF2566 at 84.523 kb on + strand, within GFF2566 at 84.523 kb on + strand, within GFF2566 at 84.524 kb on - strand, within GFF2566 at 84.527 kb on + strand, within GFF2566 at 84.527 kb on + strand, within GFF2566 at 84.528 kb on - strand, within GFF2566 at 84.531 kb on + strand, within GFF2566 at 84.804 kb on + strand at 84.804 kb on + strand at 85.049 kb on - strand at 85.057 kb on + strand at 85.082 kb on - strand at 85.682 kb on - strand, within GFF2567 at 85.822 kb on + strand, within GFF2567 at 85.823 kb on - strand, within GFF2567 at 85.869 kb on + strand, within GFF2567 at 86.219 kb on + strand at 86.220 kb on - strand at 86.220 kb on - strand at 86.220 kb on - strand at 86.220 kb on - strand at 86.310 kb on + strand at 86.310 kb on + strand at 86.600 kb on - strand at 86.614 kb on + strand at 86.615 kb on - strand at 86.661 kb on - strand at 86.779 kb on - strand, within GFF2568 at 86.846 kb on - strand, within GFF2568 at 86.920 kb on + strand, within GFF2568 at 87.038 kb on - strand, within GFF2568 at 87.039 kb on + strand, within GFF2568 at 87.040 kb on - strand, within GFF2568 at 87.172 kb on - strand, within GFF2568
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 84,038 + -1.8 84,384 - GFF2566 0.61 -0.0 84,384 - GFF2566 0.61 +0.2 84,523 + GFF2566 0.85 -0.3 84,523 + GFF2566 0.85 -1.9 84,524 - GFF2566 0.85 -2.6 84,527 + GFF2566 0.85 -0.0 84,527 + GFF2566 0.85 +1.0 84,528 - GFF2566 0.86 +1.3 84,531 + GFF2566 0.86 -0.3 84,804 + +0.0 84,804 + +0.6 85,049 - -0.7 85,057 + -2.8 85,082 - -1.7 85,682 - GFF2567 0.57 +0.6 85,822 + GFF2567 0.68 -0.7 85,823 - GFF2567 0.68 -0.4 85,869 + GFF2567 0.72 -0.4 86,219 + -1.2 86,220 - -0.4 86,220 - +0.5 86,220 - -0.4 86,220 - +0.6 86,310 + +0.6 86,310 + -0.2 86,600 - +1.6 86,614 + -0.1 86,615 - +0.1 86,661 - -0.5 86,779 - GFF2568 0.13 +0.4 86,846 - GFF2568 0.14 -2.2 86,920 + GFF2568 0.16 +1.6 87,038 - GFF2568 0.20 -0.6 87,039 + GFF2568 0.20 -0.4 87,040 - GFF2568 0.20 +0.9 87,172 - GFF2568 0.23 -0.9
Or see this region's nucleotide sequence