Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF254 and GFF255 are separated by 63 nucleotides GFF255 and GFF256 are separated by 199 nucleotides
GFF254 - 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase (EC 2.1.1.14), at 28,596 to 30,860
GFF254
GFF255 - hypothetical protein, at 30,924 to 31,052
GFF255
GFF256 - Putative arylsulfatase regulatory protein, at 31,252 to 32,547
GFF256
Position (kb)
30
31
32 Strain fitness (log2 ratio)
-2
-1
0
1 at 29.987 kb on + strand, within GFF254 at 29.987 kb on + strand, within GFF254 at 30.037 kb on + strand, within GFF254 at 30.129 kb on + strand, within GFF254 at 30.130 kb on - strand, within GFF254 at 30.210 kb on + strand, within GFF254 at 30.211 kb on - strand, within GFF254 at 30.381 kb on - strand, within GFF254 at 30.525 kb on + strand, within GFF254 at 30.525 kb on + strand, within GFF254 at 30.738 kb on - strand at 30.738 kb on - strand at 30.858 kb on + strand at 30.859 kb on - strand at 31.031 kb on - strand, within GFF255 at 31.099 kb on + strand at 31.099 kb on + strand at 31.175 kb on + strand at 31.175 kb on + strand at 31.176 kb on - strand at 31.275 kb on - strand at 31.318 kb on + strand at 31.318 kb on + strand at 31.327 kb on + strand at 31.328 kb on - strand at 31.328 kb on - strand at 31.401 kb on + strand, within GFF256 at 31.401 kb on + strand, within GFF256 at 31.403 kb on + strand, within GFF256 at 31.413 kb on + strand, within GFF256 at 31.509 kb on - strand, within GFF256 at 31.646 kb on + strand, within GFF256 at 31.646 kb on + strand, within GFF256 at 31.666 kb on - strand, within GFF256 at 31.741 kb on - strand, within GFF256 at 31.744 kb on - strand, within GFF256 at 31.744 kb on - strand, within GFF256 at 31.824 kb on + strand, within GFF256 at 31.845 kb on - strand, within GFF256 at 31.864 kb on - strand, within GFF256 at 31.875 kb on - strand, within GFF256 at 31.878 kb on + strand, within GFF256 at 32.002 kb on + strand, within GFF256 at 32.003 kb on - strand, within GFF256
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 29,987 + GFF254 0.61 +0.2 29,987 + GFF254 0.61 -1.1 30,037 + GFF254 0.64 -0.0 30,129 + GFF254 0.68 -0.0 30,130 - GFF254 0.68 +0.3 30,210 + GFF254 0.71 -0.0 30,211 - GFF254 0.71 +1.3 30,381 - GFF254 0.79 -2.1 30,525 + GFF254 0.85 -0.5 30,525 + GFF254 0.85 -0.0 30,738 - -0.9 30,738 - -1.0 30,858 + -0.1 30,859 - -0.2 31,031 - GFF255 0.83 -1.0 31,099 + +0.7 31,099 + -0.1 31,175 + -0.8 31,175 + +0.7 31,176 - +0.9 31,275 - +0.1 31,318 + -0.7 31,318 + -0.4 31,327 + -0.6 31,328 - -0.1 31,328 - -2.3 31,401 + GFF256 0.11 -0.2 31,401 + GFF256 0.11 -0.7 31,403 + GFF256 0.12 -0.7 31,413 + GFF256 0.12 -1.9 31,509 - GFF256 0.20 -0.4 31,646 + GFF256 0.30 -0.2 31,646 + GFF256 0.30 +0.1 31,666 - GFF256 0.32 -0.6 31,741 - GFF256 0.38 -0.9 31,744 - GFF256 0.38 +0.6 31,744 - GFF256 0.38 -0.5 31,824 + GFF256 0.44 +1.0 31,845 - GFF256 0.46 -1.1 31,864 - GFF256 0.47 -0.9 31,875 - GFF256 0.48 +0.0 31,878 + GFF256 0.48 -0.6 32,002 + GFF256 0.58 +0.9 32,003 - GFF256 0.58 -0.1
Or see this region's nucleotide sequence