Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF249 and GFF250 are separated by 39 nucleotides GFF250 and GFF251 are separated by 110 nucleotides GFF251 and GFF252 are separated by 15 nucleotides
GFF249 - L-lysine permease, at 23,303 to 23,923
GFF249
GFF250 - Homoserine/homoserine lactone efflux protein, at 23,963 to 24,583
GFF250
GFF251 - Lysophospholipase L2 (EC 3.1.1.5), at 24,694 to 25,710
GFF251
GFF252 - Cof protein, HD superfamily hydrolase, at 25,726 to 26,526
GFF252
Position (kb) 24
25
26 Strain fitness (log2 ratio) -2
-1
0
1
2 at 23.767 kb on - strand, within GFF249 at 23.911 kb on + strand at 23.912 kb on - strand at 23.961 kb on + strand at 23.997 kb on - strand at 24.056 kb on - strand, within GFF250 at 24.109 kb on + strand, within GFF250 at 24.110 kb on - strand, within GFF250 at 24.145 kb on - strand, within GFF250 at 24.208 kb on + strand, within GFF250 at 24.209 kb on - strand, within GFF250 at 24.224 kb on + strand, within GFF250 at 24.335 kb on - strand, within GFF250 at 24.340 kb on - strand, within GFF250 at 24.424 kb on + strand, within GFF250 at 24.424 kb on + strand, within GFF250 at 24.424 kb on + strand, within GFF250 at 24.424 kb on + strand, within GFF250 at 24.425 kb on - strand, within GFF250 at 24.429 kb on - strand, within GFF250 at 24.554 kb on + strand at 24.554 kb on + strand at 24.554 kb on + strand at 24.555 kb on - strand at 24.619 kb on + strand at 24.620 kb on - strand at 24.667 kb on + strand at 24.892 kb on + strand, within GFF251 at 24.897 kb on - strand, within GFF251 at 24.942 kb on + strand, within GFF251 at 25.093 kb on + strand, within GFF251 at 25.093 kb on + strand, within GFF251 at 25.094 kb on - strand, within GFF251 at 25.095 kb on + strand, within GFF251 at 25.168 kb on - strand, within GFF251 at 25.345 kb on + strand, within GFF251 at 25.591 kb on - strand, within GFF251 at 25.603 kb on + strand, within GFF251 at 25.779 kb on + strand at 25.871 kb on + strand, within GFF252 at 25.872 kb on - strand, within GFF252 at 25.906 kb on + strand, within GFF252 at 25.906 kb on + strand, within GFF252 at 25.962 kb on + strand, within GFF252 at 25.962 kb on + strand, within GFF252 at 26.034 kb on + strand, within GFF252 at 26.035 kb on - strand, within GFF252 at 26.112 kb on + strand, within GFF252 at 26.353 kb on - strand, within GFF252 at 26.520 kb on - strand at 26.541 kb on - strand at 26.572 kb on - strand at 26.593 kb on - strand at 26.624 kb on + strand at 26.625 kb on - strand at 26.630 kb on + strand at 26.657 kb on - strand at 26.687 kb on + strand at 26.691 kb on + strand at 26.692 kb on - strand at 26.692 kb on - strand at 26.692 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 23,767 - GFF249 0.75 +0.2 23,911 + -0.6 23,912 - +1.2 23,961 + -0.3 23,997 - +0.5 24,056 - GFF250 0.15 +0.0 24,109 + GFF250 0.24 +0.2 24,110 - GFF250 0.24 -0.2 24,145 - GFF250 0.29 +0.8 24,208 + GFF250 0.39 -0.1 24,209 - GFF250 0.40 -0.1 24,224 + GFF250 0.42 -1.6 24,335 - GFF250 0.60 -1.0 24,340 - GFF250 0.61 +2.0 24,424 + GFF250 0.74 +0.7 24,424 + GFF250 0.74 -0.2 24,424 + GFF250 0.74 -0.1 24,424 + GFF250 0.74 -0.4 24,425 - GFF250 0.74 +1.6 24,429 - GFF250 0.75 -1.9 24,554 + -0.5 24,554 + +0.2 24,554 + -0.2 24,555 - +0.1 24,619 + -0.6 24,620 - +0.2 24,667 + -0.3 24,892 + GFF251 0.19 +0.0 24,897 - GFF251 0.20 +0.1 24,942 + GFF251 0.24 +2.3 25,093 + GFF251 0.39 +1.0 25,093 + GFF251 0.39 +1.3 25,094 - GFF251 0.39 +0.5 25,095 + GFF251 0.39 -0.6 25,168 - GFF251 0.47 +0.2 25,345 + GFF251 0.64 +0.5 25,591 - GFF251 0.88 -0.2 25,603 + GFF251 0.89 +0.1 25,779 + +0.7 25,871 + GFF252 0.18 -0.9 25,872 - GFF252 0.18 -0.1 25,906 + GFF252 0.22 +1.1 25,906 + GFF252 0.22 -0.1 25,962 + GFF252 0.29 -0.3 25,962 + GFF252 0.29 +2.4 26,034 + GFF252 0.38 +0.3 26,035 - GFF252 0.39 +0.2 26,112 + GFF252 0.48 +0.5 26,353 - GFF252 0.78 +0.7 26,520 - -1.1 26,541 - +0.1 26,572 - +1.4 26,593 - +0.3 26,624 + +0.9 26,625 - -1.0 26,630 + +0.7 26,657 - +1.3 26,687 + +0.9 26,691 + +1.4 26,692 - +1.2 26,692 - +0.3 26,692 - +1.1
Or see this region's nucleotide sequence