Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2356 and GFF2357 are separated by 72 nucleotides GFF2357 and GFF2358 overlap by 4 nucleotides GFF2358 and GFF2359 are separated by 379 nucleotides GFF2359 and GFF2360 are separated by 32 nucleotides
GFF2356 - Bacterioferritin-associated ferredoxin, at 247 to 441
GFF2356
GFF2357 - Bacterioferritin, at 514 to 990
GFF2357
GFF2358 - Leader peptidase (Prepilin peptidase) (EC 3.4.23.43) / N-methyltransferase (EC 2.1.1.-), at 987 to 1,454
GFF2358
GFF2359 - SSU ribosomal protein S10p (S20e), at 1,834 to 2,145
GFF2359
GFF2360 - LSU ribosomal protein L3p (L3e), at 2,178 to 2,807
GFF2360
Position (kb) 0
1
2 Strain fitness (log2 ratio) -2
-1
0
1 at 0.250 kb on + strand at 0.264 kb on + strand at 0.271 kb on - strand, within GFF2356 at 0.511 kb on - strand at 0.550 kb on - strand at 0.586 kb on + strand, within GFF2357 at 0.681 kb on + strand, within GFF2357 at 0.685 kb on + strand, within GFF2357 at 0.685 kb on + strand, within GFF2357 at 0.685 kb on + strand, within GFF2357 at 0.686 kb on - strand, within GFF2357 at 0.686 kb on - strand, within GFF2357 at 0.686 kb on - strand, within GFF2357 at 0.687 kb on + strand, within GFF2357 at 0.687 kb on + strand, within GFF2357 at 0.717 kb on - strand, within GFF2357 at 0.833 kb on - strand, within GFF2357 at 0.853 kb on + strand, within GFF2357 at 0.867 kb on + strand, within GFF2357 at 0.976 kb on - strand at 1.021 kb on - strand at 1.069 kb on - strand, within GFF2358 at 1.190 kb on - strand, within GFF2358 at 1.394 kb on - strand, within GFF2358 at 1.394 kb on - strand, within GFF2358 at 1.405 kb on - strand, within GFF2358 at 1.405 kb on - strand, within GFF2358 at 1.490 kb on + strand at 1.497 kb on - strand at 1.497 kb on - strand at 1.518 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 250 + -0.4 264 + -0.7 271 - GFF2356 0.12 +0.4 511 - -0.4 550 - -0.5 586 + GFF2357 0.15 +0.6 681 + GFF2357 0.35 -0.7 685 + GFF2357 0.36 +0.1 685 + GFF2357 0.36 -0.5 685 + GFF2357 0.36 -0.1 686 - GFF2357 0.36 -1.1 686 - GFF2357 0.36 -0.2 686 - GFF2357 0.36 -1.0 687 + GFF2357 0.36 -0.1 687 + GFF2357 0.36 +0.7 717 - GFF2357 0.43 +1.6 833 - GFF2357 0.67 -0.1 853 + GFF2357 0.71 -1.5 867 + GFF2357 0.74 +0.1 976 - -0.1 1,021 - -0.4 1,069 - GFF2358 0.18 -0.9 1,190 - GFF2358 0.43 -1.4 1,394 - GFF2358 0.87 +0.2 1,394 - GFF2358 0.87 +0.1 1,405 - GFF2358 0.89 +0.4 1,405 - GFF2358 0.89 -2.0 1,490 + +0.0 1,497 - -0.8 1,497 - +0.1 1,518 - -0.4
Or see this region's nucleotide sequence