Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2243 and GFF2244 are separated by 14 nucleotides GFF2244 and GFF2245 are separated by 14 nucleotides GFF2245 and GFF2246 are separated by 85 nucleotides GFF2246 and GFF2247 are separated by 193 nucleotides GFF2247 and GFF2248 overlap by 30 nucleotides GFF2248 and GFF2249 are separated by 0 nucleotides GFF2249 and GFF2250 are separated by 46 nucleotides
GFF2243 - GNAT family acetyltransferase YiiD potentially involved in tRNA processing, at 37,617 to 38,558
GFF2243
GFF2244 - FIG074102: hypothetical protein, at 38,573 to 39,001
GFF2244
GFF2245 - Sea40, at 39,016 to 39,327
GFF2245
GFF2246 - Lipase, GDXG family, at 39,413 to 40,342
GFF2246
GFF2247 - FIG01046414: hypothetical protein, at 40,536 to 40,769
GFF2247
GFF2248 - FIG00642734: hypothetical protein, at 40,740 to 40,871
GFF2248
GFF2249 - Putative merR family bacterial regulatory protein, at 40,872 to 41,162
GFF2249
GFF2250 - formate dehydrogenase formation protein FdhE, at 41,209 to 42,138
GFF2250
Position (kb)
39
40
41 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 38.507 kb on - strand at 38.567 kb on - strand at 38.598 kb on - strand at 38.614 kb on + strand at 38.626 kb on + strand, within GFF2244 at 38.658 kb on - strand, within GFF2244 at 38.658 kb on - strand, within GFF2244 at 38.847 kb on - strand, within GFF2244 at 38.969 kb on - strand at 39.036 kb on - strand at 39.038 kb on + strand at 39.038 kb on + strand at 39.038 kb on + strand at 39.075 kb on + strand, within GFF2245 at 39.094 kb on - strand, within GFF2245 at 39.170 kb on + strand, within GFF2245 at 39.195 kb on + strand, within GFF2245 at 39.281 kb on + strand, within GFF2245 at 39.440 kb on + strand at 39.440 kb on + strand at 39.441 kb on - strand at 39.441 kb on - strand at 39.441 kb on - strand at 39.616 kb on + strand, within GFF2246 at 39.617 kb on - strand, within GFF2246 at 39.700 kb on + strand, within GFF2246 at 39.702 kb on + strand, within GFF2246 at 39.702 kb on + strand, within GFF2246 at 39.702 kb on + strand, within GFF2246 at 39.702 kb on + strand, within GFF2246 at 39.702 kb on + strand, within GFF2246 at 39.702 kb on + strand, within GFF2246 at 39.703 kb on - strand, within GFF2246 at 39.744 kb on + strand, within GFF2246 at 39.744 kb on + strand, within GFF2246 at 39.745 kb on - strand, within GFF2246 at 39.765 kb on + strand, within GFF2246 at 39.766 kb on - strand, within GFF2246 at 39.766 kb on - strand, within GFF2246 at 39.802 kb on - strand, within GFF2246 at 39.802 kb on - strand, within GFF2246 at 39.946 kb on + strand, within GFF2246 at 39.950 kb on - strand, within GFF2246 at 39.974 kb on - strand, within GFF2246 at 40.011 kb on + strand, within GFF2246 at 40.111 kb on - strand, within GFF2246 at 40.114 kb on - strand, within GFF2246 at 40.114 kb on - strand, within GFF2246 at 40.186 kb on + strand, within GFF2246 at 40.187 kb on - strand, within GFF2246 at 40.344 kb on + strand at 40.344 kb on + strand at 40.344 kb on + strand at 40.485 kb on - strand at 40.504 kb on - strand at 40.505 kb on + strand at 40.624 kb on - strand, within GFF2247 at 40.624 kb on - strand, within GFF2247 at 40.629 kb on - strand, within GFF2247 at 40.811 kb on + strand, within GFF2248 at 40.850 kb on + strand, within GFF2248 at 40.869 kb on + strand at 40.870 kb on - strand at 40.908 kb on - strand, within GFF2249 at 40.956 kb on - strand, within GFF2249 at 40.964 kb on + strand, within GFF2249 at 41.107 kb on + strand, within GFF2249 at 41.143 kb on + strand at 41.144 kb on - strand at 41.144 kb on - strand at 41.181 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 38,507 - -0.2 38,567 - +1.6 38,598 - +0.7 38,614 + -0.1 38,626 + GFF2244 0.12 +1.7 38,658 - GFF2244 0.20 -0.1 38,658 - GFF2244 0.20 -0.1 38,847 - GFF2244 0.64 -1.3 38,969 - +0.0 39,036 - -0.4 39,038 + +0.4 39,038 + +0.7 39,038 + +0.3 39,075 + GFF2245 0.19 +0.4 39,094 - GFF2245 0.25 -0.3 39,170 + GFF2245 0.49 -0.3 39,195 + GFF2245 0.57 -0.3 39,281 + GFF2245 0.85 +0.6 39,440 + +0.5 39,440 + +0.1 39,441 - +0.1 39,441 - +1.4 39,441 - +0.5 39,616 + GFF2246 0.22 +1.7 39,617 - GFF2246 0.22 +3.1 39,700 + GFF2246 0.31 +0.1 39,702 + GFF2246 0.31 +0.3 39,702 + GFF2246 0.31 -0.3 39,702 + GFF2246 0.31 +1.3 39,702 + GFF2246 0.31 -1.1 39,702 + GFF2246 0.31 +0.5 39,702 + GFF2246 0.31 +0.3 39,703 - GFF2246 0.31 -0.2 39,744 + GFF2246 0.36 +0.8 39,744 + GFF2246 0.36 -0.4 39,745 - GFF2246 0.36 +0.4 39,765 + GFF2246 0.38 +0.9 39,766 - GFF2246 0.38 +0.8 39,766 - GFF2246 0.38 +0.1 39,802 - GFF2246 0.42 -0.2 39,802 - GFF2246 0.42 +0.3 39,946 + GFF2246 0.57 +0.7 39,950 - GFF2246 0.58 +0.6 39,974 - GFF2246 0.60 +0.5 40,011 + GFF2246 0.64 +0.0 40,111 - GFF2246 0.75 -1.5 40,114 - GFF2246 0.75 -0.5 40,114 - GFF2246 0.75 -0.4 40,186 + GFF2246 0.83 +0.8 40,187 - GFF2246 0.83 +1.5 40,344 + +0.1 40,344 + +1.1 40,344 + +0.7 40,485 - +0.8 40,504 - +0.5 40,505 + +1.4 40,624 - GFF2247 0.38 +0.6 40,624 - GFF2247 0.38 +0.5 40,629 - GFF2247 0.40 +1.0 40,811 + GFF2248 0.54 -0.5 40,850 + GFF2248 0.83 -0.2 40,869 + -0.2 40,870 - -2.1 40,908 - GFF2249 0.12 -1.8 40,956 - GFF2249 0.29 +0.2 40,964 + GFF2249 0.32 -1.7 41,107 + GFF2249 0.81 +0.1 41,143 + +0.8 41,144 - +0.2 41,144 - -0.1 41,181 + -0.4
Or see this region's nucleotide sequence