Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF219 and GFF220 are separated by 10 nucleotides GFF220 and GFF221 overlap by 4 nucleotides GFF221 and GFF222 are separated by 29 nucleotides
GFF219 - Citrate lyase alpha chain (EC 4.1.3.6), at 216,690 to 218,210
GFF219
GFF220 - Citrate lyase beta chain (EC 4.1.3.6), at 218,221 to 219,090
GFF220
GFF221 - Citrate lyase beta chain (EC 4.1.3.6), at 219,087 to 219,380
GFF221
GFF222 - [Citrate [pro-3S]-lyase] ligase (EC 6.2.1.22), at 219,410 to 220,441
GFF222
Position (kb) 218
219
220 Strain fitness (log2 ratio) -2
-1
0
1
2 at 217.528 kb on - strand, within GFF219 at 217.528 kb on - strand, within GFF219 at 217.633 kb on + strand, within GFF219 at 217.890 kb on - strand, within GFF219 at 217.923 kb on + strand, within GFF219 at 218.158 kb on - strand at 218.196 kb on + strand at 218.227 kb on + strand at 218.360 kb on + strand, within GFF220 at 218.360 kb on + strand, within GFF220 at 218.404 kb on + strand, within GFF220 at 218.405 kb on - strand, within GFF220 at 218.432 kb on - strand, within GFF220 at 218.439 kb on + strand, within GFF220 at 218.446 kb on - strand, within GFF220 at 218.497 kb on - strand, within GFF220 at 218.889 kb on + strand, within GFF220 at 218.918 kb on + strand, within GFF220 at 218.919 kb on - strand, within GFF220 at 218.948 kb on - strand, within GFF220 at 218.974 kb on + strand, within GFF220 at 218.975 kb on - strand, within GFF220 at 219.072 kb on + strand at 219.073 kb on - strand at 219.073 kb on - strand at 219.385 kb on + strand at 219.386 kb on - strand at 219.482 kb on + strand at 219.497 kb on - strand at 219.497 kb on - strand at 219.508 kb on - strand at 219.634 kb on - strand, within GFF222 at 219.683 kb on - strand, within GFF222 at 219.802 kb on + strand, within GFF222 at 219.804 kb on + strand, within GFF222 at 219.805 kb on - strand, within GFF222 at 219.805 kb on - strand, within GFF222
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 217,528 - GFF219 0.55 +0.5 217,528 - GFF219 0.55 -0.0 217,633 + GFF219 0.62 -0.7 217,890 - GFF219 0.79 +0.1 217,923 + GFF219 0.81 -0.2 218,158 - -0.2 218,196 + +0.1 218,227 + +0.7 218,360 + GFF220 0.16 -1.0 218,360 + GFF220 0.16 +0.6 218,404 + GFF220 0.21 -0.4 218,405 - GFF220 0.21 -0.5 218,432 - GFF220 0.24 -1.0 218,439 + GFF220 0.25 -0.3 218,446 - GFF220 0.26 -0.4 218,497 - GFF220 0.32 +0.2 218,889 + GFF220 0.77 -0.6 218,918 + GFF220 0.80 -1.4 218,919 - GFF220 0.80 +0.3 218,948 - GFF220 0.84 -0.4 218,974 + GFF220 0.87 +0.2 218,975 - GFF220 0.87 -2.2 219,072 + -0.7 219,073 - -0.8 219,073 - +0.5 219,385 + -0.1 219,386 - -0.4 219,482 + +1.1 219,497 - -0.2 219,497 - -0.4 219,508 - -0.4 219,634 - GFF222 0.22 +1.9 219,683 - GFF222 0.26 -0.7 219,802 + GFF222 0.38 +0.0 219,804 + GFF222 0.38 -1.6 219,805 - GFF222 0.38 +0.1 219,805 - GFF222 0.38 -0.2
Or see this region's nucleotide sequence